USE OF A SLAM TRANSFECTED VERO CELL LINE TO ISOLATE AND CHARACTERIZE MARINE MAMMAL MORBILLIVIRUSES USING AN EXPERIMENTAL FERRET MODEL

Two ferrets (Mustela putorius furo) were experimentally infected with phocine distemper virus (PDV), from the 1988 seal epizootic in Europe, in order to determine whether the stable transfected Vero cell line (Vero.DogSLAMtag) expressing canine “signaling lymphocyte activation molecules” (SLAM; CD15...

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Veröffentlicht in:	Journal of wildlife diseases 2008-07, Vol.44 (3), p.600-611
Hauptverfasser:	Nielsen, Ole, Smith, Greg, Weingartl, Hana, Lair, Stéphane, Measures, Lena
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, CD cell culture Cell Line - virology Cercopithecus aethiops Cetacea Cytopathogenic Effect, Viral disease diagnosis Distemper - pathology Distemper - virology Distemper Virus, Phocine - isolation & purification Distemper Virus, Phocine - pathogenicity Ferret ferrets Ferrets - virology isolation Male marine mammal marine mammals Morbillivirus Mustela putorius Mustela putorius furo PDV phocine distemper Phocine distemper virus Receptors, Cell Surface reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary RT-PCR Signaling Lymphocytic Activation Molecule Family Member 1 SLAM transfection Vero Cells VIROLOGY virus isolation
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