Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing
In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant Enterococcus faecium isolates. Pyrosequencing™ showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was use...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2008-09, Vol.27 (9), p.873-878 |
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| creator | Saager, B. Rohde, H. Timmerbeil, B. S. Franke, G. Pothmann, W. Dahlke, J. Scherpe, S. Sobottka, I. Heisig, P. Horstkotte, M. A. |
| description | In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant
Enterococcus faecium
isolates. Pyrosequencing™ showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in
E. faecium
as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing™ being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance. |
| doi_str_mv | 10.1007/s10096-008-0514-6 |
| format | Article |
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Enterococcus faecium
isolates. Pyrosequencing™ showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in
E. faecium
as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing™ being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-008-0514-6</identifier><identifier>PMID: 18421487</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Acetamides - pharmacology ; Adult ; Anti-Bacterial Agents - pharmacology ; Antibacterial agents ; Antibiotic resistance ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Concise Article ; Drug Resistance, Multiple, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field ; Enterococcus faecium - drug effects ; Enterococcus faecium - genetics ; Enterococcus faecium - isolation & purification ; Female ; Genes, Bacterial ; Genes, rRNA ; Gram-Positive Bacterial Infections - microbiology ; Humans ; Infectious diseases ; Internal Medicine ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - complications ; Linezolid ; Medical Microbiology ; Medical sciences ; Microbial Sensitivity Tests ; Monitoring methods ; Mucositis - complications ; Mutation ; Oxazolidinones - pharmacology ; Pancreatitis, Acute Necrotizing - complications ; Pharmacology. Drug treatments ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA ; Vancomycin - pharmacology ; Vancomycin Resistance - genetics</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2008-09, Vol.27 (9), p.873-878</ispartof><rights>Springer-Verlag 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-b2a373ec51a36d84b1b25d94570acbb2234a0732fb2b099a7320961690de3d3</citedby><cites>FETCH-LOGICAL-c399t-b2a373ec51a36d84b1b25d94570acbb2234a0732fb2b099a7320961690de3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10096-008-0514-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10096-008-0514-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20605589$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18421487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saager, B.</creatorcontrib><creatorcontrib>Rohde, H.</creatorcontrib><creatorcontrib>Timmerbeil, B. S.</creatorcontrib><creatorcontrib>Franke, G.</creatorcontrib><creatorcontrib>Pothmann, W.</creatorcontrib><creatorcontrib>Dahlke, J.</creatorcontrib><creatorcontrib>Scherpe, S.</creatorcontrib><creatorcontrib>Sobottka, I.</creatorcontrib><creatorcontrib>Heisig, P.</creatorcontrib><creatorcontrib>Horstkotte, M. A.</creatorcontrib><title>Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant
Enterococcus faecium
isolates. Pyrosequencing™ showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in
E. faecium
as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing™ being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance.</description><subject>Acetamides - pharmacology</subject><subject>Adult</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial agents</subject><subject>Antibiotic resistance</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Concise Article</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Enterococcus faecium - drug effects</subject><subject>Enterococcus faecium - genetics</subject><subject>Enterococcus faecium - isolation & purification</subject><subject>Female</subject><subject>Genes, Bacterial</subject><subject>Genes, rRNA</subject><subject>Gram-Positive Bacterial Infections - microbiology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - complications</subject><subject>Linezolid</subject><subject>Medical Microbiology</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Monitoring methods</subject><subject>Mucositis - complications</subject><subject>Mutation</subject><subject>Oxazolidinones - pharmacology</subject><subject>Pancreatitis, Acute Necrotizing - complications</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Sequence Analysis, DNA</subject><subject>Vancomycin - pharmacology</subject><subject>Vancomycin Resistance - genetics</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcuKFDEUhoMoTjv6AG4kCIouorlWKksdxguMKChuQyqVajOkkpmcKqV9Ah_bNN06ILjJhfOd_1x-hB4y-oJRql9CO01HKO0JVUyS7hbaMCkUkUKL22hDjZDEaC5O0D2AS9pyeq3vohPWS85krzfo14eSgl-Tq9h_c9X5JdQIbokl4zLhFHP4WVIccQ0QYXHZBxwzXn4U_L19yrzzMZM_wQU_--ry6-f4PDed4ov3K-DJBR_XGUcoyS0B8Aoxb_GnXS0QrteQm8T2ProzuQThwfE-RZ_fnH85e0cuPr59f_bqgnhhzEIG7tpswSvmRDf2cmADV6ORSlPnh4FzIR3Vgk8DH6gxrj3bilhn6BjEKE7R04PqVS2tMix2juBDSi6HsoLtjGRMqa6Bj_8BL8tac-vMctZrxnlPG8QOkG-TQA2TvapxdnVnGbV7h-zBIdscsnuH7F740VF4HeYw3mQcLWnAkyPgwLs01bbmCH85TjuqVG8axw8ctFDehnrT4f-r_wali6rp</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Saager, B.</creator><creator>Rohde, H.</creator><creator>Timmerbeil, B. 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S. ; Franke, G. ; Pothmann, W. ; Dahlke, J. ; Scherpe, S. ; Sobottka, I. ; Heisig, P. ; Horstkotte, M. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-b2a373ec51a36d84b1b25d94570acbb2234a0732fb2b099a7320961690de3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acetamides - pharmacology</topic><topic>Adult</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial agents</topic><topic>Antibiotic resistance</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Concise Article</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Enterococcus faecium - drug effects</topic><topic>Enterococcus faecium - genetics</topic><topic>Enterococcus faecium - isolation & purification</topic><topic>Female</topic><topic>Genes, Bacterial</topic><topic>Genes, rRNA</topic><topic>Gram-Positive Bacterial Infections - microbiology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Internal Medicine</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - complications</topic><topic>Linezolid</topic><topic>Medical Microbiology</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Monitoring methods</topic><topic>Mucositis - complications</topic><topic>Mutation</topic><topic>Oxazolidinones - pharmacology</topic><topic>Pancreatitis, Acute Necrotizing - complications</topic><topic>Pharmacology. 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S.</au><au>Franke, G.</au><au>Pothmann, W.</au><au>Dahlke, J.</au><au>Scherpe, S.</au><au>Sobottka, I.</au><au>Heisig, P.</au><au>Horstkotte, M. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing</atitle><jtitle>European journal of clinical microbiology & infectious diseases</jtitle><stitle>Eur J Clin Microbiol Infect Dis</stitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>27</volume><issue>9</issue><spage>873</spage><epage>878</epage><pages>873-878</pages><issn>0934-9723</issn><eissn>1435-4373</eissn><abstract>In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant
Enterococcus faecium
isolates. Pyrosequencing™ showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in
E. faecium
as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing™ being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>18421487</pmid><doi>10.1007/s10096-008-0514-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0934-9723 |
| ispartof | European journal of clinical microbiology & infectious diseases, 2008-09, Vol.27 (9), p.873-878 |
| issn | 0934-9723 1435-4373 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Acetamides - pharmacology Adult Anti-Bacterial Agents - pharmacology Antibacterial agents Antibiotic resistance Antibiotics. Antiinfectious agents. Antiparasitic agents Biological and medical sciences Biomedical and Life Sciences Biomedicine Concise Article Drug Resistance, Multiple, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Enterococcus faecium - drug effects Enterococcus faecium - genetics Enterococcus faecium - isolation & purification Female Genes, Bacterial Genes, rRNA Gram-Positive Bacterial Infections - microbiology Humans Infectious diseases Internal Medicine Leukemia, Myelogenous, Chronic, BCR-ABL Positive - complications Linezolid Medical Microbiology Medical sciences Microbial Sensitivity Tests Monitoring methods Mucositis - complications Mutation Oxazolidinones - pharmacology Pancreatitis, Acute Necrotizing - complications Pharmacology. Drug treatments Polymerase Chain Reaction Polymorphism, Single Nucleotide Sequence Analysis, DNA Vancomycin - pharmacology Vancomycin Resistance - genetics |
| title | Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing |
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