The vimentin promoter as a tool to analyze the early events of retinoic acid-induced differentiation of cultured embryonal carcinoma cells
The vimentin gene encodes an intermediate filament protein expressed in the parietal endoderm, mesodermal, and early neural cells in vivo but by most in-vitro-cultured cells regardless of their embryonic origin. Here we show that the vimentin gene promoter is very active in F9 embryonal carcinoma ce...
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| Veröffentlicht in: | Differentiation (London) 1999-11, Vol.65 (3), p.171-180 |
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| creator | Benazzouz, A. Duprey, Philippe |
| description | The vimentin gene encodes an intermediate filament protein expressed in the parietal endoderm, mesodermal, and early neural cells in vivo but by most in-vitro-cultured cells regardless of their embryonic origin. Here we show that the vimentin gene promoter is very active in F9 embryonal carcinoma cells and increases in activity during differentiation. Using a series of 5′- deletion mutants, we provide evidence that the regions of the promoter involved in F9 cell activity are different from those previously demonstrated to be active in differentiated cell lines. Furthermore, we show that in differentiating F9 cells the activities of two different regions of the promoter are significantly enhanced. A distal region (−1710/−957) appears to contain functional binding sites for the murine Hox-A5 homeoprotein as demonstrated by band shift and footprinting experiments. A proximal region (−140/−78) contains a 30-bp repetitive sequence found in other genes activated during differentiation of F9 cells. Using band shift assays and methylation interference, we present evidence that a sequence-specific single-stranded DNA-binding protein(s) specifically interacts with the minus strand of the 30-bp sequence. |
| doi_str_mv | 10.1046/j.1432-0436.1999.6530171.x |
| format | Article |
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Here we show that the vimentin gene promoter is very active in F9 embryonal carcinoma cells and increases in activity during differentiation. Using a series of 5′- deletion mutants, we provide evidence that the regions of the promoter involved in F9 cell activity are different from those previously demonstrated to be active in differentiated cell lines. Furthermore, we show that in differentiating F9 cells the activities of two different regions of the promoter are significantly enhanced. A distal region (−1710/−957) appears to contain functional binding sites for the murine Hox-A5 homeoprotein as demonstrated by band shift and footprinting experiments. A proximal region (−140/−78) contains a 30-bp repetitive sequence found in other genes activated during differentiation of F9 cells. Using band shift assays and methylation interference, we present evidence that a sequence-specific single-stranded DNA-binding protein(s) specifically interacts with the minus strand of the 30-bp sequence.</description><identifier>ISSN: 0301-4681</identifier><identifier>EISSN: 1432-0436</identifier><identifier>DOI: 10.1046/j.1432-0436.1999.6530171.x</identifier><identifier>PMID: 10631814</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Animals ; Binding Sites ; Biological and medical sciences ; Biomarkers ; Bucladesine - pharmacology ; Carcinoma, Embryonal - genetics ; Carcinoma, Embryonal - pathology ; Cell Differentiation - drug effects ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Chloramphenicol O-Acetyltransferase - biosynthesis ; Chloramphenicol O-Acetyltransferase - genetics ; Consensus Sequence ; Cyclic AMP - pharmacology ; Cyclic AMP - physiology ; DNA Methylation - drug effects ; DNA, Single-Stranded - metabolism ; DNA-Binding Proteins - physiology ; Endoderm - cytology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - drug effects ; Gene Expression Regulation, Neoplastic - drug effects ; Genes, Reporter ; Homeodomain Proteins - physiology ; Mice ; Molecular and cellular biology ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - genetics ; Phosphoproteins - physiology ; Promoter Regions, Genetic ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - genetics ; Regulatory Sequences, Nucleic Acid ; Second Messenger Systems - drug effects ; Sequence Deletion ; Transfection ; Tretinoin - pharmacology ; Vimentin - biosynthesis ; Vimentin - genetics</subject><ispartof>Differentiation (London), 1999-11, Vol.65 (3), p.171-180</ispartof><rights>1999 International Society of Differentiation</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3921-621bbce537f1abafdc888a7c05fffa4da412875c4d365a1e89a31f5ed6b91c123</citedby><cites>FETCH-LOGICAL-c3921-621bbce537f1abafdc888a7c05fffa4da412875c4d365a1e89a31f5ed6b91c123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1432-0436.1999.6530171.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1046/j.1432-0436.1999.6530171.x$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,1416,3548,27923,27924,45573,45574,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1195922$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10631814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Benazzouz, A.</creatorcontrib><creatorcontrib>Duprey, Philippe</creatorcontrib><title>The vimentin promoter as a tool to analyze the early events of retinoic acid-induced differentiation of cultured embryonal carcinoma cells</title><title>Differentiation (London)</title><addtitle>Differentiation</addtitle><description>The vimentin gene encodes an intermediate filament protein expressed in the parietal endoderm, mesodermal, and early neural cells in vivo but by most in-vitro-cultured cells regardless of their embryonic origin. Here we show that the vimentin gene promoter is very active in F9 embryonal carcinoma cells and increases in activity during differentiation. Using a series of 5′- deletion mutants, we provide evidence that the regions of the promoter involved in F9 cell activity are different from those previously demonstrated to be active in differentiated cell lines. Furthermore, we show that in differentiating F9 cells the activities of two different regions of the promoter are significantly enhanced. A distal region (−1710/−957) appears to contain functional binding sites for the murine Hox-A5 homeoprotein as demonstrated by band shift and footprinting experiments. A proximal region (−140/−78) contains a 30-bp repetitive sequence found in other genes activated during differentiation of F9 cells. Using band shift assays and methylation interference, we present evidence that a sequence-specific single-stranded DNA-binding protein(s) specifically interacts with the minus strand of the 30-bp sequence.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Bucladesine - pharmacology</subject><subject>Carcinoma, Embryonal - genetics</subject><subject>Carcinoma, Embryonal - pathology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Chloramphenicol O-Acetyltransferase - biosynthesis</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Consensus Sequence</subject><subject>Cyclic AMP - pharmacology</subject><subject>Cyclic AMP - physiology</subject><subject>DNA Methylation - drug effects</subject><subject>DNA, Single-Stranded - metabolism</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Endoderm - cytology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Genes, Reporter</subject><subject>Homeodomain Proteins - physiology</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - genetics</subject><subject>Phosphoproteins - physiology</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Second Messenger Systems - drug effects</subject><subject>Sequence Deletion</subject><subject>Transfection</subject><subject>Tretinoin - pharmacology</subject><subject>Vimentin - biosynthesis</subject><subject>Vimentin - genetics</subject><issn>0301-4681</issn><issn>1432-0436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc1u1TAQhS1ERS-FV0AWQuySevIfFkiopVCpEpuytib2WPgqiYud3DY8Ak-No1wBy3ZjW_Y3xzPnMPYWRAqiqM73KRR5logir1Jo2zatylxADenDM7b7-_Sc7US8ToqqgVP2MoS9EKKpMnjBTkFUOTRQ7Njv2x_ED3agcbIjv_NucBN5joEjn5zr48JxxH75RXyKKKHvF06HyAfuDPcU65xVHJXViR31rEhzbY0hv2riZN24gmrup9nHNxo6v7goyRV6FYsH5Ir6PrxiJwb7QK-P-xn7fvX59uJrcvPty_XFp5tE5W0GSZyg6xSVeW0AOzRaNU2DtRKlMQYLjQVkTV2qQudViUBNizmYknTVtaAgy8_Y-003TvtzpjDJwYa1AxzJzUFWbSHqTDQR_LCByrsQPBl55-2AfpEg5JqE3MvVbrnaLdck5DEJ-RCL3xx_mbuB9H-lm_UReHcEMCjsjcdR2fCPg7Zss7Xbjxt2b3tantCBvLy-iococLkJUPT0YMnLoCyNMSbrSU1SO_uYef4AfgO9eQ</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>Benazzouz, A.</creator><creator>Duprey, Philippe</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199911</creationdate><title>The vimentin promoter as a tool to analyze the early events of retinoic acid-induced differentiation of cultured embryonal carcinoma cells</title><author>Benazzouz, A. ; Duprey, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3921-621bbce537f1abafdc888a7c05fffa4da412875c4d365a1e89a31f5ed6b91c123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Bucladesine - pharmacology</topic><topic>Carcinoma, Embryonal - genetics</topic><topic>Carcinoma, Embryonal - pathology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Chloramphenicol O-Acetyltransferase - biosynthesis</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Consensus Sequence</topic><topic>Cyclic AMP - pharmacology</topic><topic>Cyclic AMP - physiology</topic><topic>DNA Methylation - drug effects</topic><topic>DNA, Single-Stranded - metabolism</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Endoderm - cytology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Genes, Reporter</topic><topic>Homeodomain Proteins - physiology</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - genetics</topic><topic>Phosphoproteins - physiology</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Second Messenger Systems - drug effects</topic><topic>Sequence Deletion</topic><topic>Transfection</topic><topic>Tretinoin - pharmacology</topic><topic>Vimentin - biosynthesis</topic><topic>Vimentin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Benazzouz, A.</creatorcontrib><creatorcontrib>Duprey, Philippe</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Differentiation (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Benazzouz, A.</au><au>Duprey, Philippe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The vimentin promoter as a tool to analyze the early events of retinoic acid-induced differentiation of cultured embryonal carcinoma cells</atitle><jtitle>Differentiation (London)</jtitle><addtitle>Differentiation</addtitle><date>1999-11</date><risdate>1999</risdate><volume>65</volume><issue>3</issue><spage>171</spage><epage>180</epage><pages>171-180</pages><issn>0301-4681</issn><eissn>1432-0436</eissn><abstract>The vimentin gene encodes an intermediate filament protein expressed in the parietal endoderm, mesodermal, and early neural cells in vivo but by most in-vitro-cultured cells regardless of their embryonic origin. Here we show that the vimentin gene promoter is very active in F9 embryonal carcinoma cells and increases in activity during differentiation. Using a series of 5′- deletion mutants, we provide evidence that the regions of the promoter involved in F9 cell activity are different from those previously demonstrated to be active in differentiated cell lines. Furthermore, we show that in differentiating F9 cells the activities of two different regions of the promoter are significantly enhanced. A distal region (−1710/−957) appears to contain functional binding sites for the murine Hox-A5 homeoprotein as demonstrated by band shift and footprinting experiments. A proximal region (−140/−78) contains a 30-bp repetitive sequence found in other genes activated during differentiation of F9 cells. Using band shift assays and methylation interference, we present evidence that a sequence-specific single-stranded DNA-binding protein(s) specifically interacts with the minus strand of the 30-bp sequence.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>10631814</pmid><doi>10.1046/j.1432-0436.1999.6530171.x</doi><tpages>10</tpages></addata></record> |
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| ispartof | Differentiation (London), 1999-11, Vol.65 (3), p.171-180 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); Wiley Online Library All Journals |
| subjects | Animals Binding Sites Biological and medical sciences Biomarkers Bucladesine - pharmacology Carcinoma, Embryonal - genetics Carcinoma, Embryonal - pathology Cell Differentiation - drug effects Cell differentiation, maturation, development, hematopoiesis Cell physiology Chloramphenicol O-Acetyltransferase - biosynthesis Chloramphenicol O-Acetyltransferase - genetics Consensus Sequence Cyclic AMP - pharmacology Cyclic AMP - physiology DNA Methylation - drug effects DNA, Single-Stranded - metabolism DNA-Binding Proteins - physiology Endoderm - cytology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Neoplastic - drug effects Genes, Reporter Homeodomain Proteins - physiology Mice Molecular and cellular biology Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Phosphoproteins - physiology Promoter Regions, Genetic Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Regulatory Sequences, Nucleic Acid Second Messenger Systems - drug effects Sequence Deletion Transfection Tretinoin - pharmacology Vimentin - biosynthesis Vimentin - genetics |
| title | The vimentin promoter as a tool to analyze the early events of retinoic acid-induced differentiation of cultured embryonal carcinoma cells |
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