Purification and characterization of recombinant murine endostatin in E. coli

Endostatin, a carboxyl-terminal fragment of collagen XVIII is known as an anti-angiogenic agent, that specifically inhibits the proliferation of endothelial cell and the growth of several primary tumor. We report here the purification and characterization of the recombinant murine endostatin (rmEndo...

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Veröffentlicht in:	Experimental & molecular medicine 1999-12, Vol.31 (4), p.197-202
Hauptverfasser:	You, W K, So, S H, Lee, H, Park, S Y, Yoon, M R, Chang, S I, Kim, H K, Joe, Y A, Hong, Y K, Chung, S I
Format:	Artikel
Sprache:	eng
Schlagworte:	Angiogenesis Inhibitors - genetics Angiogenesis Inhibitors - isolation & purification Angiogenesis Inhibitors - pharmacology Animals Blotting, Western Cattle Cell Movement - drug effects Chick Embryo Chorion - drug effects Chorion - pathology Circular Dichroism Collagen - genetics Collagen - isolation & purification Collagen - pharmacology Collagen Type XVIII Electrophoresis, Polyacrylamide Gel Endostatins Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Escherichia coli - genetics Fibroblast Growth Factor 2 - pharmacology Mice Neovascularization, Physiologic - drug effects Peptide Fragments - genetics Peptide Fragments - isolation & purification Peptide Fragments - pharmacology Protein Folding Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - pharmacology Solubility Yeasts - genetics
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