A Mouse Serine/Threonine Kinase Homologous to C. elegans UNC51 Functions in Parallel Fiber Formation of Cerebellar Granule Neurons
The formation of the cerebellar circuitry depends on the outgrowth of connections between the two principal classes of neurons, granule neurons and Purkinje neurons. To identify genes that function in axon outgrowth, we have isolated a mouse homolog of C. elegans UNC51, which is required for axon fo...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1999-12, Vol.24 (4), p.833-846 |
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| creator | Tomoda, Toshifumi Bhatt, Rupal S Kuroyanagi, Hidehito Shirasawa, Takuji Hatten, Mary E |
| description | The formation of the cerebellar circuitry depends on the outgrowth of connections between the two principal classes of neurons, granule neurons and Purkinje neurons. To identify genes that function in axon outgrowth, we have isolated a mouse homolog of
C. elegans UNC51, which is required for axon formation, and tested its function in cerebellar granule neurons. Murine
Unc51.1 encodes a novel serine/threonine kinase and is expressed in granule cells in the cerebellar cortex. Retroviral infection of immature granule cells with a dominant negative
Unc51.1 results in inhibition of neurite outgrowth in vitro and in vivo. Moreover, infected neurons fail to express TAG-1 or neuron-specific β-tubulin, suggesting that development is arrested prior to this initial step of differentiation. Thus,
Unc51.1 signals the program of gene expression leading to the formation of granule cell axons. |
| doi_str_mv | 10.1016/S0896-6273(00)81031-4 |
| format | Article |
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C. elegans UNC51, which is required for axon formation, and tested its function in cerebellar granule neurons. Murine
Unc51.1 encodes a novel serine/threonine kinase and is expressed in granule cells in the cerebellar cortex. Retroviral infection of immature granule cells with a dominant negative
Unc51.1 results in inhibition of neurite outgrowth in vitro and in vivo. Moreover, infected neurons fail to express TAG-1 or neuron-specific β-tubulin, suggesting that development is arrested prior to this initial step of differentiation. Thus,
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Unc51.1 encodes a novel serine/threonine kinase and is expressed in granule cells in the cerebellar cortex. Retroviral infection of immature granule cells with a dominant negative
Unc51.1 results in inhibition of neurite outgrowth in vitro and in vivo. Moreover, infected neurons fail to express TAG-1 or neuron-specific β-tubulin, suggesting that development is arrested prior to this initial step of differentiation. Thus,
Unc51.1 signals the program of gene expression leading to the formation of granule cell axons.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Caenorhabditis elegans - physiology</subject><subject>Caenorhabditis elegans Proteins</subject><subject>Cell Differentiation - physiology</subject><subject>Cerebellum - cytology</subject><subject>Cerebellum - growth & development</subject><subject>Cytoplasmic Granules - physiology</subject><subject>Genetic Vectors</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nerve Fibers - physiology</subject><subject>Neural Pathways - cytology</subject><subject>Neural Pathways - growth & development</subject><subject>Neural Pathways - physiology</subject><subject>Neurites - physiology</subject><subject>Neurons - physiology</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Plasmids - genetics</subject><subject>Protein-Serine-Threonine Kinases - physiology</subject><subject>Retroviridae - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Unc-51.1 protein</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFSEUhonR2Gv1J2hYGV1My9fAsDLNxNsaazVpuyYMc6ZiGKgwY9Jtf7nc3sa464qv5z0HeBB6S8kRJVQeX5JOy0YyxT8Q8rGjhNNGPEMbSrRqBNX6Odr8Qw7Qq1J-EUJFq-lLdECJZEILtUH3J_hbWgvgS8g-wvHVzwwp1hn-6qOt-2dpTiHdVAYvCfdHGALc2Fjw9UXfUrxdo1t8qmsf8Q-bbQgQ8NYPkPE25dnuDnGacA8ZBgjBZnyabVwD4AtYc02-Ri8mGwq8eRwP0fX281V_1px_P_3Sn5w3rm3V0gg1Dpx3wDrtlJPtRIdpEnJoJ8XoaKXSTreWO8YG24lBWeImJuSkldVu7Cg_RO_3dW9z-r1CWczsi9tdKUJ9npGaa8Z49yRIlZCqE7yC7R50OZWSYTK32c823xlKzM6SebBkdgoMIebBkhE19-6xwTrMMP6X2mupwKc9APU__njIpjgP0cHoM7jFjMk_0eIvShyiBg</recordid><startdate>19991201</startdate><enddate>19991201</enddate><creator>Tomoda, Toshifumi</creator><creator>Bhatt, Rupal S</creator><creator>Kuroyanagi, Hidehito</creator><creator>Shirasawa, Takuji</creator><creator>Hatten, Mary E</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19991201</creationdate><title>A Mouse Serine/Threonine Kinase Homologous to C. elegans UNC51 Functions in Parallel Fiber Formation of Cerebellar Granule Neurons</title><author>Tomoda, Toshifumi ; Bhatt, Rupal S ; Kuroyanagi, Hidehito ; Shirasawa, Takuji ; Hatten, Mary E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-47db338e289c7c65f1bff46b5f721da679c95a3c22ba84b7a0cf246f97a9cd813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Caenorhabditis elegans - physiology</topic><topic>Caenorhabditis elegans Proteins</topic><topic>Cell Differentiation - physiology</topic><topic>Cerebellum - cytology</topic><topic>Cerebellum - growth & development</topic><topic>Cytoplasmic Granules - physiology</topic><topic>Genetic Vectors</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nerve Fibers - physiology</topic><topic>Neural Pathways - cytology</topic><topic>Neural Pathways - growth & development</topic><topic>Neural Pathways - physiology</topic><topic>Neurites - physiology</topic><topic>Neurons - physiology</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>Plasmids - genetics</topic><topic>Protein-Serine-Threonine Kinases - physiology</topic><topic>Retroviridae - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Unc-51.1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomoda, Toshifumi</creatorcontrib><creatorcontrib>Bhatt, Rupal S</creatorcontrib><creatorcontrib>Kuroyanagi, Hidehito</creatorcontrib><creatorcontrib>Shirasawa, Takuji</creatorcontrib><creatorcontrib>Hatten, Mary E</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomoda, Toshifumi</au><au>Bhatt, Rupal S</au><au>Kuroyanagi, Hidehito</au><au>Shirasawa, Takuji</au><au>Hatten, Mary E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Mouse Serine/Threonine Kinase Homologous to C. elegans UNC51 Functions in Parallel Fiber Formation of Cerebellar Granule Neurons</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>1999-12-01</date><risdate>1999</risdate><volume>24</volume><issue>4</issue><spage>833</spage><epage>846</epage><pages>833-846</pages><issn>0896-6273</issn><eissn>1097-4199</eissn><abstract>The formation of the cerebellar circuitry depends on the outgrowth of connections between the two principal classes of neurons, granule neurons and Purkinje neurons. To identify genes that function in axon outgrowth, we have isolated a mouse homolog of
C. elegans UNC51, which is required for axon formation, and tested its function in cerebellar granule neurons. Murine
Unc51.1 encodes a novel serine/threonine kinase and is expressed in granule cells in the cerebellar cortex. Retroviral infection of immature granule cells with a dominant negative
Unc51.1 results in inhibition of neurite outgrowth in vitro and in vivo. Moreover, infected neurons fail to express TAG-1 or neuron-specific β-tubulin, suggesting that development is arrested prior to this initial step of differentiation. Thus,
Unc51.1 signals the program of gene expression leading to the formation of granule cell axons.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10624947</pmid><doi>10.1016/S0896-6273(00)81031-4</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives (Open Access); EZB Electronic Journals Library |
| subjects | Amino Acid Sequence Animals Caenorhabditis elegans - physiology Caenorhabditis elegans Proteins Cell Differentiation - physiology Cerebellum - cytology Cerebellum - growth & development Cytoplasmic Granules - physiology Genetic Vectors Immunohistochemistry In Situ Hybridization Mice Molecular Sequence Data Mutation Nerve Fibers - physiology Neural Pathways - cytology Neural Pathways - growth & development Neural Pathways - physiology Neurites - physiology Neurons - physiology Phenotype Phylogeny Plasmids - genetics Protein-Serine-Threonine Kinases - physiology Retroviridae - genetics Reverse Transcriptase Polymerase Chain Reaction Unc-51.1 protein |
| title | A Mouse Serine/Threonine Kinase Homologous to C. elegans UNC51 Functions in Parallel Fiber Formation of Cerebellar Granule Neurons |
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