Characterization of In Vivo Developmental Chromosome Fragmentation Intermediates in E. crassus

Ligation-mediated PCR was used to characterize intermediates in the fragmentation/de novo telomere addition process that occurs during sexual reproduction in the ciliate E. crassus. Fragmentation generates ends with 6-base, 3′ overhangs that have 5′-phosphate and 3′-hydroxyl groups. These intermedia...

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Veröffentlicht in:	Molecular cell 1999-11, Vol.4 (5), p.695-704
1. Verfasser:	Klobutcher, Lawrence A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cell Nucleus - enzymology Cell Nucleus - genetics Chromosome Breakage - genetics Chromosomes - chemistry Chromosomes - genetics Chromosomes - metabolism Conserved Sequence - genetics DNA Ligases - metabolism DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Protozoan - metabolism DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics DNA, Single-Stranded - metabolism Euplotes - cytology Euplotes - enzymology Euplotes - genetics Euplotes - growth & development Euplotes crassus Evolution, Molecular Micronucleus, Germline - enzymology Micronucleus, Germline - genetics Models, Genetic Polymerase Chain Reaction - methods Regulatory Sequences, Nucleic Acid - genetics Reproduction Retroelements - genetics Space life sciences Telomerase - metabolism Telomere - chemistry Telomere - genetics Telomere - metabolism
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