Induction of blood–brain barrier properties in immortalized bovine brain endothelial cells by astrocytic factors
The blood–brain barrier (B-BB) protects the free passage of substances into the brain and maintains the homeostasis of the central nervous system. It is commonly accepted that astrocytes surrounding brain endothelial cells influence the B-BB formation and the exhibition of B-BB function of capillari...
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| Veröffentlicht in: | Neuroscience research 1999-11, Vol.35 (2), p.155-164 |
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| creator | Sobue, Kazuya Yamamoto, Naoki Yoneda, Kazuhiro Hodgson, Mark E Yamashiro, Kyoko Tsuruoka, Nobuo Tsuda, Takako Katsuya, Hirotada Miura, Yutaka Asai, Kiyofumi Kato, Taiji |
| description | The blood–brain barrier (B-BB) protects the free passage of substances into the brain and maintains the homeostasis of the central nervous system. It is commonly accepted that astrocytes surrounding brain endothelial cells influence the B-BB formation and the exhibition of B-BB function of capillaries. To begin the in vitro study on the B-BB, it is essential to obtain a homogenous and sufficient supply of brain endothelial cells as well as astrocytes. We thus immortalized the bovine brain endothelial cell (BBEC) by transfection of the SV40 large T antigen and obtained a single clone, t-BBEC-117, which retained the brain endothelial cell phenotype. Astrocyte in co-culture was found to tighten the intercellular contacts of the immortal cells resulting in a reduced
l-glucose permeability, and its conditioned medium (CM) augmented a B-BB phenotype, alkaline phosphatase (ALP) activity. Among known astrocytic factors, only fibroblast growth factor-basic (bFGF) could mimic the actions of astrocytes as measured by
l-glucose permeability and ALP activity. Moreover, anti-bFGF antibody canceled 90% of ALP activation by astrocyte CM. Basic FGF, however, failed to induce other B-BB phenotypes such as the expressions of multidrug resistance (mdr) and glucose transporter (GLUT-1) genes. These data suggest that bFGF is one of the most plausible astrocytic factors to induce the B-BB properties of immortal brain endothelial cells together with some unknown factors in the astrocyte CM. |
| doi_str_mv | 10.1016/S0168-0102(99)00079-6 |
| format | Article |
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l-glucose permeability, and its conditioned medium (CM) augmented a B-BB phenotype, alkaline phosphatase (ALP) activity. Among known astrocytic factors, only fibroblast growth factor-basic (bFGF) could mimic the actions of astrocytes as measured by
l-glucose permeability and ALP activity. Moreover, anti-bFGF antibody canceled 90% of ALP activation by astrocyte CM. Basic FGF, however, failed to induce other B-BB phenotypes such as the expressions of multidrug resistance (mdr) and glucose transporter (GLUT-1) genes. These data suggest that bFGF is one of the most plausible astrocytic factors to induce the B-BB properties of immortal brain endothelial cells together with some unknown factors in the astrocyte CM.</description><identifier>ISSN: 0168-0102</identifier><identifier>EISSN: 1872-8111</identifier><identifier>DOI: 10.1016/S0168-0102(99)00079-6</identifier><identifier>PMID: 10616919</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Alkaline Phosphatase - metabolism ; Animals ; Antigens, Differentiation - biosynthesis ; Antigens, Viral, Tumor - genetics ; Astrocyte ; Astrocytes - cytology ; Astrocytes - metabolism ; ATP-Binding Cassette, Sub-Family B, Member 1 - genetics ; Base Sequence ; Blood-Brain Barrier - drug effects ; Blood-Brain Barrier - physiology ; Blood–brain barrier ; Brain - blood supply ; Brain - cytology ; Brain - drug effects ; Brain - metabolism ; Cattle ; Cell Membrane Permeability - drug effects ; Cell Membrane Permeability - physiology ; Cells, Cultured ; Coculture Techniques ; Culture Media, Conditioned - metabolism ; Endothelial cell ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Enzyme Activation - drug effects ; Fibroblast growth factor ; Glucose - metabolism ; Growth Substances - metabolism ; Growth Substances - pharmacology ; Immortalization ; Molecular Sequence Data ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Tight junction ; Transfection</subject><ispartof>Neuroscience research, 1999-11, Vol.35 (2), p.155-164</ispartof><rights>1999 Elsevier Science Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-d221dc6996ba820005f94b5d842c45e9b92bef5556e947619c8fbb8cd6b738d33</citedby><cites>FETCH-LOGICAL-c427t-d221dc6996ba820005f94b5d842c45e9b92bef5556e947619c8fbb8cd6b738d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-0102(99)00079-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10616919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sobue, Kazuya</creatorcontrib><creatorcontrib>Yamamoto, Naoki</creatorcontrib><creatorcontrib>Yoneda, Kazuhiro</creatorcontrib><creatorcontrib>Hodgson, Mark E</creatorcontrib><creatorcontrib>Yamashiro, Kyoko</creatorcontrib><creatorcontrib>Tsuruoka, Nobuo</creatorcontrib><creatorcontrib>Tsuda, Takako</creatorcontrib><creatorcontrib>Katsuya, Hirotada</creatorcontrib><creatorcontrib>Miura, Yutaka</creatorcontrib><creatorcontrib>Asai, Kiyofumi</creatorcontrib><creatorcontrib>Kato, Taiji</creatorcontrib><title>Induction of blood–brain barrier properties in immortalized bovine brain endothelial cells by astrocytic factors</title><title>Neuroscience research</title><addtitle>Neurosci Res</addtitle><description>The blood–brain barrier (B-BB) protects the free passage of substances into the brain and maintains the homeostasis of the central nervous system. It is commonly accepted that astrocytes surrounding brain endothelial cells influence the B-BB formation and the exhibition of B-BB function of capillaries. To begin the in vitro study on the B-BB, it is essential to obtain a homogenous and sufficient supply of brain endothelial cells as well as astrocytes. We thus immortalized the bovine brain endothelial cell (BBEC) by transfection of the SV40 large T antigen and obtained a single clone, t-BBEC-117, which retained the brain endothelial cell phenotype. Astrocyte in co-culture was found to tighten the intercellular contacts of the immortal cells resulting in a reduced
l-glucose permeability, and its conditioned medium (CM) augmented a B-BB phenotype, alkaline phosphatase (ALP) activity. Among known astrocytic factors, only fibroblast growth factor-basic (bFGF) could mimic the actions of astrocytes as measured by
l-glucose permeability and ALP activity. Moreover, anti-bFGF antibody canceled 90% of ALP activation by astrocyte CM. Basic FGF, however, failed to induce other B-BB phenotypes such as the expressions of multidrug resistance (mdr) and glucose transporter (GLUT-1) genes. These data suggest that bFGF is one of the most plausible astrocytic factors to induce the B-BB properties of immortal brain endothelial cells together with some unknown factors in the astrocyte CM.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Antigens, Differentiation - biosynthesis</subject><subject>Antigens, Viral, Tumor - genetics</subject><subject>Astrocyte</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - metabolism</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - genetics</subject><subject>Base Sequence</subject><subject>Blood-Brain Barrier - drug effects</subject><subject>Blood-Brain Barrier - physiology</subject><subject>Blood–brain barrier</subject><subject>Brain - blood supply</subject><subject>Brain - cytology</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Cattle</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cell Membrane Permeability - physiology</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Culture Media, Conditioned - metabolism</subject><subject>Endothelial cell</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Enzyme Activation - drug effects</subject><subject>Fibroblast growth factor</subject><subject>Glucose - metabolism</subject><subject>Growth Substances - metabolism</subject><subject>Growth Substances - pharmacology</subject><subject>Immortalization</subject><subject>Molecular Sequence Data</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tight junction</subject><subject>Transfection</subject><issn>0168-0102</issn><issn>1872-8111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9O3DAQhy1UVJZtH6GVTxUcAraTOPYJIQQtEhIH6Nnyn4lwlcSL7UXanngH3pAnwbtBVW9cPJL1zcxvPoS-UXJCCeWnd-URFaGEHUl5TAjpZMX30IKKjlWCUvoJLf4hB-gwpT8FqmVTf0YHlHDKJZULFK8nt7bZhwmHHpshBPf6_GKi9hM2OkYPEa9iWEHMHhIuv34cQ8x68H_BYROe_AR45mFyIT_A4PWALQxDwmaDdcox2E32Fvfa5hDTF7Tf6yHB1_e6RL-vLu8vflU3tz-vL85vKtuwLleOMeosl5IbLVjJ3vayMa0TDbNNC9JIZqBv25aDbDpOpRW9McI6brpauLpeoh_z3JL_cQ0pq9GnbS49QVgnxWUtmOBbsJ1BG0NKEXq1in7UcaMoUVvZaidbbU0qKdVOtuKl7_v7grUZwf3XNdstwNkMQDnzqahUyXqYLDgfwWblgv9gxRvwvpH8</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>Sobue, Kazuya</creator><creator>Yamamoto, Naoki</creator><creator>Yoneda, Kazuhiro</creator><creator>Hodgson, Mark E</creator><creator>Yamashiro, Kyoko</creator><creator>Tsuruoka, Nobuo</creator><creator>Tsuda, Takako</creator><creator>Katsuya, Hirotada</creator><creator>Miura, Yutaka</creator><creator>Asai, Kiyofumi</creator><creator>Kato, Taiji</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991101</creationdate><title>Induction of blood–brain barrier properties in immortalized bovine brain endothelial cells by astrocytic factors</title><author>Sobue, Kazuya ; Yamamoto, Naoki ; Yoneda, Kazuhiro ; Hodgson, Mark E ; Yamashiro, Kyoko ; Tsuruoka, Nobuo ; Tsuda, Takako ; Katsuya, Hirotada ; Miura, Yutaka ; Asai, Kiyofumi ; Kato, Taiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-d221dc6996ba820005f94b5d842c45e9b92bef5556e947619c8fbb8cd6b738d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Antigens, Differentiation - biosynthesis</topic><topic>Antigens, Viral, Tumor - genetics</topic><topic>Astrocyte</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - metabolism</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - genetics</topic><topic>Base Sequence</topic><topic>Blood-Brain Barrier - drug effects</topic><topic>Blood-Brain Barrier - physiology</topic><topic>Blood–brain barrier</topic><topic>Brain - blood supply</topic><topic>Brain - cytology</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>Cattle</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Cell Membrane Permeability - physiology</topic><topic>Cells, Cultured</topic><topic>Coculture Techniques</topic><topic>Culture Media, Conditioned - metabolism</topic><topic>Endothelial cell</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Enzyme Activation - drug effects</topic><topic>Fibroblast growth factor</topic><topic>Glucose - metabolism</topic><topic>Growth Substances - metabolism</topic><topic>Growth Substances - pharmacology</topic><topic>Immortalization</topic><topic>Molecular Sequence Data</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tight junction</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sobue, Kazuya</creatorcontrib><creatorcontrib>Yamamoto, Naoki</creatorcontrib><creatorcontrib>Yoneda, Kazuhiro</creatorcontrib><creatorcontrib>Hodgson, Mark E</creatorcontrib><creatorcontrib>Yamashiro, Kyoko</creatorcontrib><creatorcontrib>Tsuruoka, Nobuo</creatorcontrib><creatorcontrib>Tsuda, Takako</creatorcontrib><creatorcontrib>Katsuya, Hirotada</creatorcontrib><creatorcontrib>Miura, Yutaka</creatorcontrib><creatorcontrib>Asai, Kiyofumi</creatorcontrib><creatorcontrib>Kato, Taiji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sobue, Kazuya</au><au>Yamamoto, Naoki</au><au>Yoneda, Kazuhiro</au><au>Hodgson, Mark E</au><au>Yamashiro, Kyoko</au><au>Tsuruoka, Nobuo</au><au>Tsuda, Takako</au><au>Katsuya, Hirotada</au><au>Miura, Yutaka</au><au>Asai, Kiyofumi</au><au>Kato, Taiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of blood–brain barrier properties in immortalized bovine brain endothelial cells by astrocytic factors</atitle><jtitle>Neuroscience research</jtitle><addtitle>Neurosci Res</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>35</volume><issue>2</issue><spage>155</spage><epage>164</epage><pages>155-164</pages><issn>0168-0102</issn><eissn>1872-8111</eissn><abstract>The blood–brain barrier (B-BB) protects the free passage of substances into the brain and maintains the homeostasis of the central nervous system. It is commonly accepted that astrocytes surrounding brain endothelial cells influence the B-BB formation and the exhibition of B-BB function of capillaries. To begin the in vitro study on the B-BB, it is essential to obtain a homogenous and sufficient supply of brain endothelial cells as well as astrocytes. We thus immortalized the bovine brain endothelial cell (BBEC) by transfection of the SV40 large T antigen and obtained a single clone, t-BBEC-117, which retained the brain endothelial cell phenotype. Astrocyte in co-culture was found to tighten the intercellular contacts of the immortal cells resulting in a reduced
l-glucose permeability, and its conditioned medium (CM) augmented a B-BB phenotype, alkaline phosphatase (ALP) activity. Among known astrocytic factors, only fibroblast growth factor-basic (bFGF) could mimic the actions of astrocytes as measured by
l-glucose permeability and ALP activity. Moreover, anti-bFGF antibody canceled 90% of ALP activation by astrocyte CM. Basic FGF, however, failed to induce other B-BB phenotypes such as the expressions of multidrug resistance (mdr) and glucose transporter (GLUT-1) genes. These data suggest that bFGF is one of the most plausible astrocytic factors to induce the B-BB properties of immortal brain endothelial cells together with some unknown factors in the astrocyte CM.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>10616919</pmid><doi>10.1016/S0168-0102(99)00079-6</doi><tpages>10</tpages></addata></record> |
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| ispartof | Neuroscience research, 1999-11, Vol.35 (2), p.155-164 |
| issn | 0168-0102 1872-8111 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Alkaline Phosphatase - metabolism Animals Antigens, Differentiation - biosynthesis Antigens, Viral, Tumor - genetics Astrocyte Astrocytes - cytology Astrocytes - metabolism ATP-Binding Cassette, Sub-Family B, Member 1 - genetics Base Sequence Blood-Brain Barrier - drug effects Blood-Brain Barrier - physiology Blood–brain barrier Brain - blood supply Brain - cytology Brain - drug effects Brain - metabolism Cattle Cell Membrane Permeability - drug effects Cell Membrane Permeability - physiology Cells, Cultured Coculture Techniques Culture Media, Conditioned - metabolism Endothelial cell Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Enzyme Activation - drug effects Fibroblast growth factor Glucose - metabolism Growth Substances - metabolism Growth Substances - pharmacology Immortalization Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Tight junction Transfection |
| title | Induction of blood–brain barrier properties in immortalized bovine brain endothelial cells by astrocytic factors |
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