A bacmid approach to the genetic manipulation of granuloviruses

A Cydia pomonella granulovirus (CpGV) bacmid has been constructed, which allows rapid and efficient production of recombinant baculoviruses in Escherichia coli. An 8.6 kbp bacterial DNA cassette derived from the AcMNPV Bac-to-Bac ® system was ligated into a unique PacI restriction site within an int...

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Veröffentlicht in:	Journal of virological methods 2008-09, Vol.152 (1), p.56-62
Hauptverfasser:	Hilton, Sally, Kemp, Elizabeth, Keane, Gary, Winstanley, Doreen
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacmid Baculoviridae - genetics Baculoviridae - metabolism Baculovirus Biological and medical sciences Cell Line CpGV Cydia pomonella Cysteine Endopeptidases - metabolism Drosophila Escherichia coli Escherichia coli - virology Fat Body - enzymology Fat Body - virology Fundamental and applied biological sciences. Psychology Genes, Viral - genetics Genetic Engineering - methods Granulovirus Granulovirus - genetics Green Fluorescent Proteins - genetics Larva - enzymology Larva - virology Microbiology Moths - cytology Moths - virology Recombinant Proteins - genetics Techniques used in virology Virology
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