Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood

ABSTRACT Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35–40 cycles yielded visible band...

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Veröffentlicht in:	Microbiology and immunology 2008-07, Vol.52 (7), p.375-382
Hauptverfasser:	Moriyama, Kosei, Ando, Chie, Tashiro, Kosuke, Kuhara, Satoru, Okamura, Seiichi, Nakano, Shuji, Takagi, Yasumitsu, Miki, Takeyoshi, Nakashima, Yoshiyuki, Hirakawa, Hideki
Format:	Artikel
Sprache:	eng
Schlagworte:	16S ribosomal RNA bacteremia Bacteremia - diagnosis Bacteria - classification Bacteria - genetics Bacteria - isolation & purification blood Blood - microbiology DNA, Bacterial - genetics DNA, Ribosomal - genetics Humans polymerase chain reaction Polymerase Chain Reaction - methods RNA, Ribosomal, 16S - genetics
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