A Proteomic Study of S-Nitrosylation in the Rat Cardiac Proteins in Vitro

Protein S-nitrosylation in the heart tissue has been implicated in several patho (physiological) processes. However, specific protein targets for S-nitrosylation remain largely unknown. In this study, the rat cardiac proteins were incubated in vitro with S-nitrosoglutathione (GSNO), a biologically e...

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Veröffentlicht in:	Biological & pharmaceutical bulletin 2008/08/01, Vol.31(8), pp.1536-1540
Hauptverfasser:	Shi, Qiang, Feng, JinHong, Qu, HaiBin, Cheng, Yi-Yu
Format:	Artikel
Sprache:	eng
Schlagworte:	adenylate kinase Adenylate Kinase - metabolism Animals biotin switch method Blotting, Western Databases, Factual Dithiothreitol - pharmacology Electrophoresis, Gel, Two-Dimensional Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism In Vitro Techniques Male Muscle Proteins - genetics Muscle Proteins - metabolism Myocardium - metabolism Nitric Oxide Donors - pharmacology Nitroso Compounds - metabolism Proteomics - methods Rats Rats, Sprague-Dawley S-nitrosoglutathione S-Nitrosoglutathione - chemical synthesis S-Nitrosoglutathione - pharmacology S-nitrosoproteome S-nitrosylation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfhydryl Reagents - pharmacology
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