Parvovirus B19 infection of bone marrow in systemic sclerosis patients
To investigate the prevalence of human parvovirus B19 (B19) infection in the bone marrow of systemic sclerosis (SSc) patients. Twenty-one consecutive SSc patients and 15 sex- and age-matched subjects without immunological rheumatic diseases were studied for: (i) the presence of circulating anti-B19...
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| Veröffentlicht in: | Clinical and experimental rheumatology 1999-11, Vol.17 (6), p.718-720 |
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| creator | FERRI, C ZAKRZEWSKA, K LONGOMBARDO, G GIUGGIOLI, D STORINO, F. A. A PASERO, G AZZI, A |
| description | To investigate the prevalence of human parvovirus B19 (B19) infection in the bone marrow of systemic sclerosis (SSc) patients.
Twenty-one consecutive SSc patients and 15 sex- and age-matched subjects without immunological rheumatic diseases were studied for: (i) the presence of circulating anti-B19 antibodies (anti-B19 IgG and IgM type and anti-B19 NS1 IgG) detected by means of standard methodologies, and (ii) B19 genomic sequences in sera and bone marrow biopsy specimens using a nested-PCR technique.
The presence of B19 DNA was demonstrated in a significant percentage of bone marrow biopsies from SSc patients (12/21; 57%) and was never detected in the control group (p < 0.01). In no case was the B19 viremia observed, while serum anti-B19 NS1 antibodies, possible markers of B19 persistent infection, were more frequently detected in SSc patients than in controls (33% vs 13%). SSc patients with bone marrow B19 infection showed a shorter mean disease duration than B19-negative patients (5.6 +/- 4.2 vs 12.7 +/- 7.8 yrs; p < 0.01).
This is the first demonstration of bone marrow B19 infection in a significant percentage of SSc patients. The possible etiopathogenetic role of B19 should be verified in a larger patients series and further investigated by means of molecular biology studies. |
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Twenty-one consecutive SSc patients and 15 sex- and age-matched subjects without immunological rheumatic diseases were studied for: (i) the presence of circulating anti-B19 antibodies (anti-B19 IgG and IgM type and anti-B19 NS1 IgG) detected by means of standard methodologies, and (ii) B19 genomic sequences in sera and bone marrow biopsy specimens using a nested-PCR technique.
The presence of B19 DNA was demonstrated in a significant percentage of bone marrow biopsies from SSc patients (12/21; 57%) and was never detected in the control group (p < 0.01). In no case was the B19 viremia observed, while serum anti-B19 NS1 antibodies, possible markers of B19 persistent infection, were more frequently detected in SSc patients than in controls (33% vs 13%). SSc patients with bone marrow B19 infection showed a shorter mean disease duration than B19-negative patients (5.6 +/- 4.2 vs 12.7 +/- 7.8 yrs; p < 0.01).
This is the first demonstration of bone marrow B19 infection in a significant percentage of SSc patients. The possible etiopathogenetic role of B19 should be verified in a larger patients series and further investigated by means of molecular biology studies.</description><identifier>ISSN: 0392-856X</identifier><identifier>EISSN: 1593-098X</identifier><identifier>PMID: 10609071</identifier><language>eng</language><publisher>Pisa: Clinical and Experimental Rheumatology</publisher><subject>Adult ; Aged ; Antibodies, Viral - blood ; Biological and medical sciences ; Bone Marrow - virology ; Bone Marrow Diseases - blood ; Bone Marrow Diseases - etiology ; Bone Marrow Diseases - virology ; DNA, Viral - analysis ; Female ; Humans ; Immunoglobulin G - blood ; Italy ; Male ; Medical sciences ; Middle Aged ; Parvoviridae Infections - blood ; Parvoviridae Infections - etiology ; Parvoviridae Infections - virology ; Parvovirus B19, Human - genetics ; Parvovirus B19, Human - immunology ; Parvovirus B19, Human - isolation & purification ; Polymerase Chain Reaction ; Prevalence ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; Scleroderma, Systemic - blood ; Scleroderma, Systemic - complications ; Scleroderma, Systemic - virology</subject><ispartof>Clinical and experimental rheumatology, 1999-11, Vol.17 (6), p.718-720</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1195543$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10609071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FERRI, C</creatorcontrib><creatorcontrib>ZAKRZEWSKA, K</creatorcontrib><creatorcontrib>LONGOMBARDO, G</creatorcontrib><creatorcontrib>GIUGGIOLI, D</creatorcontrib><creatorcontrib>STORINO, F. A. A</creatorcontrib><creatorcontrib>PASERO, G</creatorcontrib><creatorcontrib>AZZI, A</creatorcontrib><title>Parvovirus B19 infection of bone marrow in systemic sclerosis patients</title><title>Clinical and experimental rheumatology</title><addtitle>Clin Exp Rheumatol</addtitle><description>To investigate the prevalence of human parvovirus B19 (B19) infection in the bone marrow of systemic sclerosis (SSc) patients.
Twenty-one consecutive SSc patients and 15 sex- and age-matched subjects without immunological rheumatic diseases were studied for: (i) the presence of circulating anti-B19 antibodies (anti-B19 IgG and IgM type and anti-B19 NS1 IgG) detected by means of standard methodologies, and (ii) B19 genomic sequences in sera and bone marrow biopsy specimens using a nested-PCR technique.
The presence of B19 DNA was demonstrated in a significant percentage of bone marrow biopsies from SSc patients (12/21; 57%) and was never detected in the control group (p < 0.01). In no case was the B19 viremia observed, while serum anti-B19 NS1 antibodies, possible markers of B19 persistent infection, were more frequently detected in SSc patients than in controls (33% vs 13%). SSc patients with bone marrow B19 infection showed a shorter mean disease duration than B19-negative patients (5.6 +/- 4.2 vs 12.7 +/- 7.8 yrs; p < 0.01).
This is the first demonstration of bone marrow B19 infection in a significant percentage of SSc patients. The possible etiopathogenetic role of B19 should be verified in a larger patients series and further investigated by means of molecular biology studies.</description><subject>Adult</subject><subject>Aged</subject><subject>Antibodies, Viral - blood</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow - virology</subject><subject>Bone Marrow Diseases - blood</subject><subject>Bone Marrow Diseases - etiology</subject><subject>Bone Marrow Diseases - virology</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Italy</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Parvoviridae Infections - blood</subject><subject>Parvoviridae Infections - etiology</subject><subject>Parvoviridae Infections - virology</subject><subject>Parvovirus B19, Human - genetics</subject><subject>Parvovirus B19, Human - immunology</subject><subject>Parvovirus B19, Human - isolation & purification</subject><subject>Polymerase Chain Reaction</subject><subject>Prevalence</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>Scleroderma, Systemic - blood</subject><subject>Scleroderma, Systemic - complications</subject><subject>Scleroderma, Systemic - virology</subject><issn>0392-856X</issn><issn>1593-098X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFz8FLwzAYBfAgiqvTf0FyEG-FpGma5qjDqTDQg8Ju5UuaQKRtar52sv_eiRNP7_B-PHgnJONSi5zpentKMiZ0kdey2i7IBeIHY0UlK3VOFpxVTDPFM7J-hbSLu5BmpPdc0zB4Z6cQBxo9NXFwtIeU4tehoLjHyfXBUrSdSxED0hGm4IYJL8mZhw7d1TGX5H398LZ6yjcvj8-ru00-FqKa8lZ4AOEkGGukMbo2inMPqlYlb4uSGeVNzaRqpStKq0Fb0FDWphRS-EIIsSS3v7tjip-zw6npA1rXdTC4OGNTaaEKpX_g9RHOpndtM6ZwOLJv_p4fwM0RAFrofILBBvx3XEtZCvEN2sxjGg</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>FERRI, C</creator><creator>ZAKRZEWSKA, K</creator><creator>LONGOMBARDO, G</creator><creator>GIUGGIOLI, D</creator><creator>STORINO, F. A. A</creator><creator>PASERO, G</creator><creator>AZZI, A</creator><general>Clinical and Experimental Rheumatology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19991101</creationdate><title>Parvovirus B19 infection of bone marrow in systemic sclerosis patients</title><author>FERRI, C ; ZAKRZEWSKA, K ; LONGOMBARDO, G ; GIUGGIOLI, D ; STORINO, F. A. A ; PASERO, G ; AZZI, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p236t-d3faa3e5abcb5bb98b711fa78741d240b7fb8057d5e24c9a9ca9a48b4353f2333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antibodies, Viral - blood</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow - virology</topic><topic>Bone Marrow Diseases - blood</topic><topic>Bone Marrow Diseases - etiology</topic><topic>Bone Marrow Diseases - virology</topic><topic>DNA, Viral - analysis</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin G - blood</topic><topic>Italy</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Parvoviridae Infections - blood</topic><topic>Parvoviridae Infections - etiology</topic><topic>Parvoviridae Infections - virology</topic><topic>Parvovirus B19, Human - genetics</topic><topic>Parvovirus B19, Human - immunology</topic><topic>Parvovirus B19, Human - isolation & purification</topic><topic>Polymerase Chain Reaction</topic><topic>Prevalence</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>Scleroderma, Systemic - blood</topic><topic>Scleroderma, Systemic - complications</topic><topic>Scleroderma, Systemic - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FERRI, C</creatorcontrib><creatorcontrib>ZAKRZEWSKA, K</creatorcontrib><creatorcontrib>LONGOMBARDO, G</creatorcontrib><creatorcontrib>GIUGGIOLI, D</creatorcontrib><creatorcontrib>STORINO, F. A. A</creatorcontrib><creatorcontrib>PASERO, G</creatorcontrib><creatorcontrib>AZZI, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental rheumatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FERRI, C</au><au>ZAKRZEWSKA, K</au><au>LONGOMBARDO, G</au><au>GIUGGIOLI, D</au><au>STORINO, F. A. A</au><au>PASERO, G</au><au>AZZI, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Parvovirus B19 infection of bone marrow in systemic sclerosis patients</atitle><jtitle>Clinical and experimental rheumatology</jtitle><addtitle>Clin Exp Rheumatol</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>17</volume><issue>6</issue><spage>718</spage><epage>720</epage><pages>718-720</pages><issn>0392-856X</issn><eissn>1593-098X</eissn><abstract>To investigate the prevalence of human parvovirus B19 (B19) infection in the bone marrow of systemic sclerosis (SSc) patients.
Twenty-one consecutive SSc patients and 15 sex- and age-matched subjects without immunological rheumatic diseases were studied for: (i) the presence of circulating anti-B19 antibodies (anti-B19 IgG and IgM type and anti-B19 NS1 IgG) detected by means of standard methodologies, and (ii) B19 genomic sequences in sera and bone marrow biopsy specimens using a nested-PCR technique.
The presence of B19 DNA was demonstrated in a significant percentage of bone marrow biopsies from SSc patients (12/21; 57%) and was never detected in the control group (p < 0.01). In no case was the B19 viremia observed, while serum anti-B19 NS1 antibodies, possible markers of B19 persistent infection, were more frequently detected in SSc patients than in controls (33% vs 13%). SSc patients with bone marrow B19 infection showed a shorter mean disease duration than B19-negative patients (5.6 +/- 4.2 vs 12.7 +/- 7.8 yrs; p < 0.01).
This is the first demonstration of bone marrow B19 infection in a significant percentage of SSc patients. The possible etiopathogenetic role of B19 should be verified in a larger patients series and further investigated by means of molecular biology studies.</abstract><cop>Pisa</cop><pub>Clinical and Experimental Rheumatology</pub><pmid>10609071</pmid><tpages>3</tpages></addata></record> |
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| subjects | Adult Aged Antibodies, Viral - blood Biological and medical sciences Bone Marrow - virology Bone Marrow Diseases - blood Bone Marrow Diseases - etiology Bone Marrow Diseases - virology DNA, Viral - analysis Female Humans Immunoglobulin G - blood Italy Male Medical sciences Middle Aged Parvoviridae Infections - blood Parvoviridae Infections - etiology Parvoviridae Infections - virology Parvovirus B19, Human - genetics Parvovirus B19, Human - immunology Parvovirus B19, Human - isolation & purification Polymerase Chain Reaction Prevalence Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis Scleroderma, Systemic - blood Scleroderma, Systemic - complications Scleroderma, Systemic - virology |
| title | Parvovirus B19 infection of bone marrow in systemic sclerosis patients |
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