Relevance of Molecular Markers in the Analysis of Malaria Parasite Populations
Malaria is one of the main human public health problems in the tropical world and is possibly becoming an emerging disease too in regions where it has been controlled. It has been an excellent model in the area of molecular studies, with scientific validation of techniques, application of data mainl...
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| Veröffentlicht in: | Transboundary and emerging diseases 2008-08, Vol.55 (5-6), p.226-232 |
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| creator | Arez, A.P do Rosário, V.E |
| description | Malaria is one of the main human public health problems in the tropical world and is possibly becoming an emerging disease too in regions where it has been controlled. It has been an excellent model in the area of molecular studies, with scientific validation of techniques, application of data mainly in studies of parasite diversity and information on a number of different aspects associated with infection and disease. The transfer of the gathered knowledge and experience in malaria to other infections is of great use and we briefly review a number of molecular markers, methodologies and techniques mostly used for Plasmodium detection, as well as identification or characterization of parasite populations. Selection of appropriate techniques depends on the questions raised and the studies' objectives - the antigen-coding genes, microsatellite loci and drug-resistance associated markers being the three most analysed classes of markers. The need of validation and standardization of laboratory protocols is addressed and discussed as it may determine the comparison of data between different studies and laboratories, with relevance in field-collected samples or studies. |
| doi_str_mv | 10.1111/j.1865-1682.2008.01025.x |
| format | Article |
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It has been an excellent model in the area of molecular studies, with scientific validation of techniques, application of data mainly in studies of parasite diversity and information on a number of different aspects associated with infection and disease. The transfer of the gathered knowledge and experience in malaria to other infections is of great use and we briefly review a number of molecular markers, methodologies and techniques mostly used for Plasmodium detection, as well as identification or characterization of parasite populations. Selection of appropriate techniques depends on the questions raised and the studies' objectives - the antigen-coding genes, microsatellite loci and drug-resistance associated markers being the three most analysed classes of markers. The need of validation and standardization of laboratory protocols is addressed and discussed as it may determine the comparison of data between different studies and laboratories, with relevance in field-collected samples or studies.</description><subject>Animal diseases</subject><subject>Animals</subject><subject>antigens</subject><subject>biomarkers</subject><subject>diagnosis</subject><subject>Diagnosis, Differential</subject><subject>disease detection</subject><subject>drug resistance</subject><subject>emerging diseases</subject><subject>genes</subject><subject>genetic markers</subject><subject>Genetic Markers - genetics</subject><subject>Genetic Variation</subject><subject>geographical distribution</subject><subject>Humans</subject><subject>laboratory techniques</subject><subject>literature reviews</subject><subject>Malaria</subject><subject>Malaria - diagnosis</subject><subject>Malaria - veterinary</subject><subject>microsatellite repeats</subject><subject>Molecular biology</subject><subject>molecular epidemiology</subject><subject>molecular markers</subject><subject>molecular sequence data</subject><subject>Parasites</subject><subject>pathogen identification</subject><subject>Plasmodium</subject><subject>Plasmodium - classification</subject><subject>Plasmodium - genetics</subject><subject>Plasmodium - isolation & purification</subject><subject>Public Health</subject><subject>Research methodology</subject><subject>Sensitivity and Specificity</subject><subject>Species Specificity</subject><issn>1865-1682</issn><issn>1865-1674</issn><issn>1865-1682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EoqXwFyDiwC3BH4kTX5BKW1pQW1a0FceR7Y7BSzZZ7AR2_z1OsyqIC_jikeaZR5p5CckYLVh6r5cFa2SVM9nwglPaFJRRXhWbB2T_vvHwj3qPPIlxSamkSlaPyV5qSKmk3CeXn7DFH7qzmPUuu-hbtGOrQ3ahwzcMMfNdNnzF7LDT7Tb6eAfpBHidLXTQ0Q-YLfp1mhl838Wn5JHTbcRnu_-A3Lw7uT46y88_nr4_OjzPbSWaKkfmTEU5q285RytkicYqWXNFnXGIDA01SqE1TjjDZamplpg21NaokiorDsir2bsO_fcR4wArHy22re6wHyNIJWpWl-U_QaakKsumTuDLv8BlP4a0dgQuGJtON9maGbKhjzGgg3XwKx22wChMycASpqPDdHSYkoG7ZGCTRp_v_KNZ4e3vwV0UCXgzAz99i9v_FsP125PjqUyCfBb4OODmXpCCBFmLuoLPl6fwgS3q4-ZsAVeJfzHzTvegvwQf4eaKUyYoVUxyIcQv3uS0eA</recordid><startdate>200808</startdate><enddate>200808</enddate><creator>Arez, A.P</creator><creator>do Rosário, V.E</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Hindawi Limited</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200808</creationdate><title>Relevance of Molecular Markers in the Analysis of Malaria Parasite Populations</title><author>Arez, A.P ; do Rosário, V.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5385-e1fb50217d22ec364ebc967290fbfee1eb0b99ecbf3fb264a0a6e025acb9409c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animal diseases</topic><topic>Animals</topic><topic>antigens</topic><topic>biomarkers</topic><topic>diagnosis</topic><topic>Diagnosis, Differential</topic><topic>disease detection</topic><topic>drug resistance</topic><topic>emerging diseases</topic><topic>genes</topic><topic>genetic markers</topic><topic>Genetic Markers - genetics</topic><topic>Genetic Variation</topic><topic>geographical distribution</topic><topic>Humans</topic><topic>laboratory techniques</topic><topic>literature reviews</topic><topic>Malaria</topic><topic>Malaria - diagnosis</topic><topic>Malaria - veterinary</topic><topic>microsatellite repeats</topic><topic>Molecular biology</topic><topic>molecular epidemiology</topic><topic>molecular markers</topic><topic>molecular sequence data</topic><topic>Parasites</topic><topic>pathogen identification</topic><topic>Plasmodium</topic><topic>Plasmodium - classification</topic><topic>Plasmodium - genetics</topic><topic>Plasmodium - isolation & purification</topic><topic>Public Health</topic><topic>Research methodology</topic><topic>Sensitivity and Specificity</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arez, A.P</creatorcontrib><creatorcontrib>do Rosário, V.E</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transboundary and emerging diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arez, A.P</au><au>do Rosário, V.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relevance of Molecular Markers in the Analysis of Malaria Parasite Populations</atitle><jtitle>Transboundary and emerging diseases</jtitle><addtitle>Transbound Emerg Dis</addtitle><date>2008-08</date><risdate>2008</risdate><volume>55</volume><issue>5-6</issue><spage>226</spage><epage>232</epage><pages>226-232</pages><issn>1865-1682</issn><issn>1865-1674</issn><eissn>1865-1682</eissn><abstract>Malaria is one of the main human public health problems in the tropical world and is possibly becoming an emerging disease too in regions where it has been controlled. It has been an excellent model in the area of molecular studies, with scientific validation of techniques, application of data mainly in studies of parasite diversity and information on a number of different aspects associated with infection and disease. The transfer of the gathered knowledge and experience in malaria to other infections is of great use and we briefly review a number of molecular markers, methodologies and techniques mostly used for Plasmodium detection, as well as identification or characterization of parasite populations. Selection of appropriate techniques depends on the questions raised and the studies' objectives - the antigen-coding genes, microsatellite loci and drug-resistance associated markers being the three most analysed classes of markers. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animal diseases Animals antigens biomarkers diagnosis Diagnosis, Differential disease detection drug resistance emerging diseases genes genetic markers Genetic Markers - genetics Genetic Variation geographical distribution Humans laboratory techniques literature reviews Malaria Malaria - diagnosis Malaria - veterinary microsatellite repeats Molecular biology molecular epidemiology molecular markers molecular sequence data Parasites pathogen identification Plasmodium Plasmodium - classification Plasmodium - genetics Plasmodium - isolation & purification Public Health Research methodology Sensitivity and Specificity Species Specificity |
| title | Relevance of Molecular Markers in the Analysis of Malaria Parasite Populations |
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