Real-Time Quantitative PCR Detection of Genetically Modified Maximizer Maize and Roundup Ready Soybean in Some Representative Foods
A fast and quantitative method was developed to detect transgenic “Maximizer” maize “event 176” (Novartis) and “Roundup Ready” soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumula...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 1999-12, Vol.47 (12), p.5261-5266 |
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| container_end_page | 5266 |
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| container_issue | 12 |
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| container_title | Journal of agricultural and food chemistry |
| container_volume | 47 |
| creator | Vaïtilingom, Marc Pijnenburg, Hans Gendre, François Brignon, Pierre |
| description | A fast and quantitative method was developed to detect transgenic “Maximizer” maize “event 176” (Novartis) and “Roundup Ready” soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of “Maximizer” maize and “Roundup Ready” soybean in some representative food products were quantified. Keywords: “Maximizer” maize “event 176”; “Roundup Ready” soybean; real-time quantitative PCR; GMO detection |
| doi_str_mv | 10.1021/jf981208v |
| format | Article |
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Agric. Food Chem</addtitle><description>A fast and quantitative method was developed to detect transgenic “Maximizer” maize “event 176” (Novartis) and “Roundup Ready” soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of “Maximizer” maize and “Roundup Ready” soybean in some representative food products were quantified. 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Psychology</subject><subject>General aspects</subject><subject>Genetic Engineering</subject><subject>Glycine max - chemistry</subject><subject>Glycine max - genetics</subject><subject>Humans</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>Plants, Genetically Modified - chemistry</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Zea mays - chemistry</subject><subject>Zea mays - genetics</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMFu1DAQhiMEokvhwAsgHwCJQ8BOYsc5ol1akFpYssvZmo3HkpfE3tpJ1eXKi-Mqq8IBydKMNJ__GX1Z9pLR94wW7MPeNJIVVN4-yhaMFzTnjMnH2YKmYS65YGfZsxj3lFLJa_o0O2NUUJHeIvvdIvT51g5Ivk_gRjvCaG-RrJctWeGI3Wi9I96QS3Q42g76_kiuvbbGoibXcGcH-wtD6lIh4DRp_eT0dCApWB_Jxh93CI5Yl9q0pMVDwIjutObCex2fZ08M9BFfnOp59uPi03b5Ob_6dvll-fEqh6qqxhwNaCObRnRScK3LgjFTNbsOSs5rXUio6oJhzTmj3AiAsqxkhXWjqTCFLE15nr2dcw_B30wYRzXY2GHfg0M_RSWaUjTpewLfzWAXfIwBjToEO0A4KkbVvXH1YDyxr06h025A_Q85K07A6xMAMekzAVxn41-uqKSQZcLyGbNxxLuHMYSfStRlzdV2vVHs62a9avlK3d_4Zuahi2rvp-CSuv_c9wfx2aQT</recordid><startdate>19991201</startdate><enddate>19991201</enddate><creator>Vaïtilingom, Marc</creator><creator>Pijnenburg, Hans</creator><creator>Gendre, François</creator><creator>Brignon, Pierre</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991201</creationdate><title>Real-Time Quantitative PCR Detection of Genetically Modified Maximizer Maize and Roundup Ready Soybean in Some Representative Foods</title><author>Vaïtilingom, Marc ; Pijnenburg, Hans ; Gendre, François ; Brignon, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a444t-efadf8996c865dd3211f49bca3557d28a4721e755105f6aa33484e79d06f283f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Cereal and baking product industries</topic><topic>DNA - isolation & purification</topic><topic>DNA Primers</topic><topic>Food industries</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Genetic Engineering</topic><topic>Glycine max - chemistry</topic><topic>Glycine max - genetics</topic><topic>Humans</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>Plants, Genetically Modified - chemistry</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Zea mays - chemistry</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vaïtilingom, Marc</creatorcontrib><creatorcontrib>Pijnenburg, Hans</creatorcontrib><creatorcontrib>Gendre, François</creatorcontrib><creatorcontrib>Brignon, Pierre</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vaïtilingom, Marc</au><au>Pijnenburg, Hans</au><au>Gendre, François</au><au>Brignon, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real-Time Quantitative PCR Detection of Genetically Modified Maximizer Maize and Roundup Ready Soybean in Some Representative Foods</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. 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| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 1999-12, Vol.47 (12), p.5261-5266 |
| issn | 0021-8561 1520-5118 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Biological and medical sciences Cereal and baking product industries DNA - isolation & purification DNA Primers Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology General aspects Genetic Engineering Glycine max - chemistry Glycine max - genetics Humans Methods of analysis, processing and quality control, regulation, standards Plants, Genetically Modified - chemistry Plants, Genetically Modified - genetics Polymerase Chain Reaction Zea mays - chemistry Zea mays - genetics |
| title | Real-Time Quantitative PCR Detection of Genetically Modified Maximizer Maize and Roundup Ready Soybean in Some Representative Foods |
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