The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice
Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and che...
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| Veröffentlicht in: | Histochemistry and cell biology 2008-08, Vol.130 (2), p.363-373 |
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| creator | Mikkelsen, H. B. Larsen, J. O. Hadberg, H. |
| description | Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and chemokines that causes influx of mononuclear cells and neutrophilic granulocytes. We subjected osteopetrotic (
op
/
op
) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80
+
, MHCII
+
, and myeloperoxidase
+
cells were quantified using stereological sampling. In +/+ mice we found that MHCII
+
cells outnumber F4/80
+
cells and that LPS injection increased the density of MHCII
+
cells temporarily but not that of F4/80
+
cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII
+
, CD169
+
, and F4/80
+
cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and
op
/
op
mice. |
| doi_str_mv | 10.1007/s00418-008-0423-x |
| format | Article |
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op
/
op
) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80
+
, MHCII
+
, and myeloperoxidase
+
cells were quantified using stereological sampling. In +/+ mice we found that MHCII
+
cells outnumber F4/80
+
cells and that LPS injection increased the density of MHCII
+
cells temporarily but not that of F4/80
+
cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII
+
, CD169
+
, and F4/80
+
cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and
op
/
op
mice.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s00418-008-0423-x</identifier><identifier>PMID: 18392842</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Antigens, Differentiation - immunology ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cellular biology ; Cytokines ; Developmental Biology ; Female ; Gastroenterology ; Histocompatibility Antigens Class II - immunology ; Inflammation - immunology ; Inflammation - pathology ; Inflammatory diseases ; Jejunum - immunology ; Jejunum - pathology ; Lipopolysaccharides - immunology ; Macrophage Activation - immunology ; Macrophages - immunology ; Mice ; Mice, Mutant Strains ; Muscle, Smooth - immunology ; Muscle, Smooth - pathology ; Original Paper ; Osteopetrosis - immunology ; Rodents</subject><ispartof>Histochemistry and cell biology, 2008-08, Vol.130 (2), p.363-373</ispartof><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-5200966e4144f155d07ed911d69d653d0f20af2113398b5b5a25b3bdc6d08e453</citedby><cites>FETCH-LOGICAL-c400t-5200966e4144f155d07ed911d69d653d0f20af2113398b5b5a25b3bdc6d08e453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00418-008-0423-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00418-008-0423-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18392842$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mikkelsen, H. B.</creatorcontrib><creatorcontrib>Larsen, J. O.</creatorcontrib><creatorcontrib>Hadberg, H.</creatorcontrib><title>The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><addtitle>Histochem Cell Biol</addtitle><description>Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and chemokines that causes influx of mononuclear cells and neutrophilic granulocytes. We subjected osteopetrotic (
op
/
op
) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80
+
, MHCII
+
, and myeloperoxidase
+
cells were quantified using stereological sampling. In +/+ mice we found that MHCII
+
cells outnumber F4/80
+
cells and that LPS injection increased the density of MHCII
+
cells temporarily but not that of F4/80
+
cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII
+
, CD169
+
, and F4/80
+
cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and
op
/
op
mice.</description><subject>Animals</subject><subject>Antigens, Differentiation - immunology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cellular biology</subject><subject>Cytokines</subject><subject>Developmental Biology</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>Histocompatibility Antigens Class II - immunology</subject><subject>Inflammation - immunology</subject><subject>Inflammation - pathology</subject><subject>Inflammatory diseases</subject><subject>Jejunum - immunology</subject><subject>Jejunum - pathology</subject><subject>Lipopolysaccharides - immunology</subject><subject>Macrophage Activation - immunology</subject><subject>Macrophages - immunology</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Muscle, Smooth - immunology</subject><subject>Muscle, Smooth - pathology</subject><subject>Original Paper</subject><subject>Osteopetrosis - immunology</subject><subject>Rodents</subject><issn>0948-6143</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU9rFTEUxYMo9rX6AdxIcOFu9N5MMp24k6JWKLip4G7ITDJ9KZNkmj_lvc_hFzaP96AgiIsQSH7nHu45hLxB-IAAlx8TAMe-AaiHs7bZPSMb5C1rEOWv52QDkvdNV1_OyHlK9wAoJGMvyRn2rWQ9Zxvy-3ZrqFNTDOtW3Rma9ikbR62nuX5Yn03K1quFupKmsqhoEzW7bKJXVJdo_V2F5kU5p7IN_hNVnlrnig9bm3KYtsbZqcqV1_ShKJ9truBjNcpF72mYaaiGYTU5hmwnWmnziryY1ZLM69N9QX5-_XJ7dd3c_Pj2_erzTTNxgNwIBiC7znDkfEYhNFwaLRF1J3UnWg0zAzUzxLaV_ShGoZgY21FPnYbecNFekPfHuWsMD6UuOjibJrMsyptQ0tDJthN9je9_IIO-R8FlBd_9Bd6HUqNaKoMCRSfEwRaPUA09pWjmYY3WqbgfEIZDr8Ox16H2Ohx6HXZV8_Y0uIzO6CfFqcgKsCOQ1kMpJj45_3vqHwU1sTg</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Mikkelsen, H. 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B.</au><au>Larsen, J. O.</au><au>Hadberg, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice</atitle><jtitle>Histochemistry and cell biology</jtitle><stitle>Histochem Cell Biol</stitle><addtitle>Histochem Cell Biol</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>130</volume><issue>2</issue><spage>363</spage><epage>373</epage><pages>363-373</pages><issn>0948-6143</issn><eissn>1432-119X</eissn><abstract>Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and chemokines that causes influx of mononuclear cells and neutrophilic granulocytes. We subjected osteopetrotic (
op
/
op
) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80
+
, MHCII
+
, and myeloperoxidase
+
cells were quantified using stereological sampling. In +/+ mice we found that MHCII
+
cells outnumber F4/80
+
cells and that LPS injection increased the density of MHCII
+
cells temporarily but not that of F4/80
+
cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII
+
, CD169
+
, and F4/80
+
cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and
op
/
op
mice.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>18392842</pmid><doi>10.1007/s00418-008-0423-x</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0948-6143 |
| ispartof | Histochemistry and cell biology, 2008-08, Vol.130 (2), p.363-373 |
| issn | 0948-6143 1432-119X |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Antigens, Differentiation - immunology Biochemistry Biomedical and Life Sciences Biomedicine Cell Biology Cellular biology Cytokines Developmental Biology Female Gastroenterology Histocompatibility Antigens Class II - immunology Inflammation - immunology Inflammation - pathology Inflammatory diseases Jejunum - immunology Jejunum - pathology Lipopolysaccharides - immunology Macrophage Activation - immunology Macrophages - immunology Mice Mice, Mutant Strains Muscle, Smooth - immunology Muscle, Smooth - pathology Original Paper Osteopetrosis - immunology Rodents |
| title | The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice |
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