Enrichment and detection of live antigen‐specific CD4+ and CD8+ T cells based on cytokine secretion
Following appropriate antigen‐specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation‐induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolat...
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| Veröffentlicht in: | European journal of immunology 1999-12, Vol.29 (12), p.4053-4059 |
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| container_end_page | 4059 |
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| container_issue | 12 |
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| container_title | European journal of immunology |
| container_volume | 29 |
| creator | Brosterhus, Helmut Brings, Susanne Leyendeckers, Heike Manz, Rudolf A. Miltenyi, Stefan Radbruch, Andreas Assenmacher, Mario Schmitz, Jürgen |
| description | Following appropriate antigen‐specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation‐induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen‐specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HLA‐A0201‐restricted influenza matrix protein peptide 58‐66‐specific CD8+ cytotoxic T lymphocytes, influenza A virus‐ and recombinant tetanus toxin C fragment‐specific Th1 cells and tetanus toxoid‐specific Th2 cells. |
| doi_str_mv | 10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C |
| format | Article |
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Based on this stimulation‐induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen‐specific CD4+ and CD8+ T lymphocytes. 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Based on this stimulation‐induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen‐specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HLA‐A0201‐restricted influenza matrix protein peptide 58‐66‐specific CD8+ cytotoxic T lymphocytes, influenza A virus‐ and recombinant tetanus toxin C fragment‐specific Th1 cells and tetanus toxoid‐specific Th2 cells.</description><subject>Affinity matrix technology</subject><subject>AIDS/HIV</subject><subject>Antigen Presentation</subject><subject>Antigens - immunology</subject><subject>Antigen‐specific T lymphocyte</subject><subject>CD4-Positive T-Lymphocytes - cytology</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - cytology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cytokines - immunology</subject><subject>Cytokine‐secretion</subject><subject>Humans</subject><subject>Immunomagnetic Separation - methods</subject><subject>Magnetic cell sorting</subject><subject>Memory T cell</subject><subject>Receptors, Antigen, T-Cell - immunology</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctu1EAQtBCILIFfQHNCiSIv3TN-zQYhBW8AS4n2QHLhQGs824YBr714vKC98Ql8I1-CjUOEhBCXbnWruqpVFQQvEOYIIJ8evSny4hhjiWGEER6h1hrlsdQLlM8iiNVicVYsw-Ly8nqcnqs5zPPVqQzzO8Hs9uxuMAPAKJQ6g4PggfcfAUAnsb4fHCAkIAGTWcDnTefshw03vTDNWqy5Z9u7thFtJWr3hYdt795z8-Pbd79l6ypnRb6MTn6h82V2Iq6E5br2ojSe12K4tPu-_eQaFp5txyPZw-BeZWrPj276YXD98vwqfx1erF4V-dlFaFUaqTBNS8gqSFMwZRKXUFYmidPSYKx0ZmylMS2RjclYZ6htaSJlK2MzGXGKVq7VYfBk4t127ecd-542zo_fmYbbnadEqyRGzAbg2wlou9b7jivadm5juj0h0BgC0RgCjW7S6CZNIZDUNNTRdqIhBPodAikCylckKR_IH998sSs3vP6DenJ9ALybAF9dzfu_pP-r_A_h2536CaRjpLU</recordid><startdate>199912</startdate><enddate>199912</enddate><creator>Brosterhus, Helmut</creator><creator>Brings, Susanne</creator><creator>Leyendeckers, Heike</creator><creator>Manz, Rudolf A.</creator><creator>Miltenyi, Stefan</creator><creator>Radbruch, Andreas</creator><creator>Assenmacher, Mario</creator><creator>Schmitz, Jürgen</creator><general>WILEY‐VCH Verlag GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199912</creationdate><title>Enrichment and detection of live antigen‐specific CD4+ and CD8+ T cells based on cytokine secretion</title><author>Brosterhus, Helmut ; Brings, Susanne ; Leyendeckers, Heike ; Manz, Rudolf A. ; Miltenyi, Stefan ; Radbruch, Andreas ; Assenmacher, Mario ; Schmitz, Jürgen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3743-77b08f0770ab65b0bfa657ba15398acf917b1eaa8e9819cba43cfac824e71c2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Affinity matrix technology</topic><topic>AIDS/HIV</topic><topic>Antigen Presentation</topic><topic>Antigens - immunology</topic><topic>Antigen‐specific T lymphocyte</topic><topic>CD4-Positive T-Lymphocytes - cytology</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - cytology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cytokines - immunology</topic><topic>Cytokine‐secretion</topic><topic>Humans</topic><topic>Immunomagnetic Separation - methods</topic><topic>Magnetic cell sorting</topic><topic>Memory T cell</topic><topic>Receptors, Antigen, T-Cell - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brosterhus, Helmut</creatorcontrib><creatorcontrib>Brings, Susanne</creatorcontrib><creatorcontrib>Leyendeckers, Heike</creatorcontrib><creatorcontrib>Manz, Rudolf A.</creatorcontrib><creatorcontrib>Miltenyi, Stefan</creatorcontrib><creatorcontrib>Radbruch, Andreas</creatorcontrib><creatorcontrib>Assenmacher, Mario</creatorcontrib><creatorcontrib>Schmitz, Jürgen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brosterhus, Helmut</au><au>Brings, Susanne</au><au>Leyendeckers, Heike</au><au>Manz, Rudolf A.</au><au>Miltenyi, Stefan</au><au>Radbruch, Andreas</au><au>Assenmacher, Mario</au><au>Schmitz, Jürgen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enrichment and detection of live antigen‐specific CD4+ and CD8+ T cells based on cytokine secretion</atitle><jtitle>European journal of immunology</jtitle><addtitle>Eur J Immunol</addtitle><date>1999-12</date><risdate>1999</risdate><volume>29</volume><issue>12</issue><spage>4053</spage><epage>4059</epage><pages>4053-4059</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><abstract>Following appropriate antigen‐specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation‐induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen‐specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HLA‐A0201‐restricted influenza matrix protein peptide 58‐66‐specific CD8+ cytotoxic T lymphocytes, influenza A virus‐ and recombinant tetanus toxin C fragment‐specific Th1 cells and tetanus toxoid‐specific Th2 cells.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH</pub><pmid>10602016</pmid><doi>10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of immunology, 1999-12, Vol.29 (12), p.4053-4059 |
| issn | 0014-2980 1521-4141 |
| language | eng |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Affinity matrix technology AIDS/HIV Antigen Presentation Antigens - immunology Antigen‐specific T lymphocyte CD4-Positive T-Lymphocytes - cytology CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - cytology CD8-Positive T-Lymphocytes - immunology Cytokines - immunology Cytokine‐secretion Humans Immunomagnetic Separation - methods Magnetic cell sorting Memory T cell Receptors, Antigen, T-Cell - immunology |
| title | Enrichment and detection of live antigen‐specific CD4+ and CD8+ T cells based on cytokine secretion |
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