The Concentration-Dependent Contractile Effect of Methylene Blue in the Human Internal Mammary Artery: A Quantitative Approach to Its Use in the Vasoplegic Syndrome
Objective: To quantitate the contractile effect of methylene blue on isolated human internal mammary artery (IMA) as used in the vasoplegic syndrome. Design: An in vitro experimental study. Setting: Cardiovascular Pharmacology Laboratory, Department of Medical Pharmacology. Participants: IMA segment...
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| Veröffentlicht in: | Journal of cardiothoracic and vascular anesthesia 2008-08, Vol.22 (4), p.560-564 |
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| creator | Ulusoy, Hasan B., MD, PhD Gul, Husamettin, MD, PhD Seyrek, Melik, MD, PhD Yildiz, Oguzhan, MD, PhD Ulku, Cunay, MD, PhD Yıldırım, Vedat, MD Kuralay, Erkan, MD Celik, Turgay, MD Yanarates, Omer, MD |
| description | Objective: To quantitate the contractile effect of methylene blue on isolated human internal mammary artery (IMA) as used in the vasoplegic syndrome. Design: An in vitro experimental study. Setting: Cardiovascular Pharmacology Laboratory, Department of Medical Pharmacology. Participants: IMA segments were used from 24 patients undergoing coronary artery bypass surgery. Interventions: The responses to methylene blue, norepinephrine, and acetylcholine were recorded isometrically by a force-displacement transducer in an isolated organ bath. Measurement and Main Results: Methylene blue (10 nmol/L-100 μmol/L) produced concentration-dependent contraction in the arteries. The maximal contraction to methylene blue was 44.2% ± 3.8% of KCl (68 mmol/L) maximum contraction; the pEC50 (−log10 of 50% effective concentration) value was 5.5 ± 0.1. Methylene blue caused an insignificant leftward shift of the concentration-response curve of norepinephrine. Acetylcholine-induced relaxation in submaximal contracted rings with phenylephrine recovered nearly 6 hours after the methylene blue challenge. Conclusion: Methylene blue caused concentration-dependent contraction in human IMAs. Furthermore, the inhibition of ACh-induced relaxation for 6 hours after the methylene blue challenge points out an additional mechanism (ie, receptor occupation). The concentration-dependent contractile effect of methylene blue justifies its use in the vasoplegic syndrome. The findings also suggest that the time course of contraction is longer than the exposure to methylene blue. |
| doi_str_mv | 10.1053/j.jvca.2008.02.018 |
| format | Article |
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Design: An in vitro experimental study. Setting: Cardiovascular Pharmacology Laboratory, Department of Medical Pharmacology. Participants: IMA segments were used from 24 patients undergoing coronary artery bypass surgery. Interventions: The responses to methylene blue, norepinephrine, and acetylcholine were recorded isometrically by a force-displacement transducer in an isolated organ bath. Measurement and Main Results: Methylene blue (10 nmol/L-100 μmol/L) produced concentration-dependent contraction in the arteries. The maximal contraction to methylene blue was 44.2% ± 3.8% of KCl (68 mmol/L) maximum contraction; the pEC50 (−log10 of 50% effective concentration) value was 5.5 ± 0.1. Methylene blue caused an insignificant leftward shift of the concentration-response curve of norepinephrine. Acetylcholine-induced relaxation in submaximal contracted rings with phenylephrine recovered nearly 6 hours after the methylene blue challenge. Conclusion: Methylene blue caused concentration-dependent contraction in human IMAs. Furthermore, the inhibition of ACh-induced relaxation for 6 hours after the methylene blue challenge points out an additional mechanism (ie, receptor occupation). The concentration-dependent contractile effect of methylene blue justifies its use in the vasoplegic syndrome. The findings also suggest that the time course of contraction is longer than the exposure to methylene blue.</description><identifier>ISSN: 1053-0770</identifier><identifier>EISSN: 1532-8422</identifier><identifier>DOI: 10.1053/j.jvca.2008.02.018</identifier><identifier>PMID: 18662631</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Aged ; Anesthesia & Perioperative Care ; contraction ; Critical Care ; Dose-Response Relationship, Drug ; human internal mammary artery ; Humans ; In Vitro Techniques ; Mammary Arteries - drug effects ; Mammary Arteries - physiology ; methylene blue ; Methylene Blue - administration & dosage ; Middle Aged ; Muscle Contraction - drug effects ; Muscle Contraction - physiology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - physiology ; Syndrome ; Vasoconstriction - drug effects ; Vasoconstriction - physiology ; vasoplegic syndrome</subject><ispartof>Journal of cardiothoracic and vascular anesthesia, 2008-08, Vol.22 (4), p.560-564</ispartof><rights>Elsevier Inc.</rights><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-e92d971889fff7513fd758c3fb99a2d020be15728c8a8b3a550e536331ee00743</citedby><cites>FETCH-LOGICAL-c409t-e92d971889fff7513fd758c3fb99a2d020be15728c8a8b3a550e536331ee00743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1053077008000505$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18662631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ulusoy, Hasan B., MD, PhD</creatorcontrib><creatorcontrib>Gul, Husamettin, MD, PhD</creatorcontrib><creatorcontrib>Seyrek, Melik, MD, PhD</creatorcontrib><creatorcontrib>Yildiz, Oguzhan, MD, PhD</creatorcontrib><creatorcontrib>Ulku, Cunay, MD, PhD</creatorcontrib><creatorcontrib>Yıldırım, Vedat, MD</creatorcontrib><creatorcontrib>Kuralay, Erkan, MD</creatorcontrib><creatorcontrib>Celik, Turgay, MD</creatorcontrib><creatorcontrib>Yanarates, Omer, MD</creatorcontrib><title>The Concentration-Dependent Contractile Effect of Methylene Blue in the Human Internal Mammary Artery: A Quantitative Approach to Its Use in the Vasoplegic Syndrome</title><title>Journal of cardiothoracic and vascular anesthesia</title><addtitle>J Cardiothorac Vasc Anesth</addtitle><description>Objective: To quantitate the contractile effect of methylene blue on isolated human internal mammary artery (IMA) as used in the vasoplegic syndrome. Design: An in vitro experimental study. Setting: Cardiovascular Pharmacology Laboratory, Department of Medical Pharmacology. Participants: IMA segments were used from 24 patients undergoing coronary artery bypass surgery. Interventions: The responses to methylene blue, norepinephrine, and acetylcholine were recorded isometrically by a force-displacement transducer in an isolated organ bath. Measurement and Main Results: Methylene blue (10 nmol/L-100 μmol/L) produced concentration-dependent contraction in the arteries. The maximal contraction to methylene blue was 44.2% ± 3.8% of KCl (68 mmol/L) maximum contraction; the pEC50 (−log10 of 50% effective concentration) value was 5.5 ± 0.1. Methylene blue caused an insignificant leftward shift of the concentration-response curve of norepinephrine. Acetylcholine-induced relaxation in submaximal contracted rings with phenylephrine recovered nearly 6 hours after the methylene blue challenge. Conclusion: Methylene blue caused concentration-dependent contraction in human IMAs. Furthermore, the inhibition of ACh-induced relaxation for 6 hours after the methylene blue challenge points out an additional mechanism (ie, receptor occupation). The concentration-dependent contractile effect of methylene blue justifies its use in the vasoplegic syndrome. The findings also suggest that the time course of contraction is longer than the exposure to methylene blue.</description><subject>Adult</subject><subject>Aged</subject><subject>Anesthesia & Perioperative Care</subject><subject>contraction</subject><subject>Critical Care</subject><subject>Dose-Response Relationship, Drug</subject><subject>human internal mammary artery</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Mammary Arteries - drug effects</subject><subject>Mammary Arteries - physiology</subject><subject>methylene blue</subject><subject>Methylene Blue - administration & dosage</subject><subject>Middle Aged</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Contraction - physiology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>Syndrome</subject><subject>Vasoconstriction - drug effects</subject><subject>Vasoconstriction - physiology</subject><subject>vasoplegic syndrome</subject><issn>1053-0770</issn><issn>1532-8422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UsFuEzEQXSEQLYUf4IB84rZhbMe7XoSQQmhppFYIteVqOd5Z4rBrB9sbKf_TD8WrRCBx4GTP03tvPPNcFK8pzCgI_m472-6NnjEAOQM2AyqfFOdUcFbKOWNP8z2zSqhrOCtexLgFoFSI-nlxRmVVsYrT8-LxfoNk6Z1Bl4JO1rvyM-7Qtbme8AyaZHskl12HJhHfkVtMm0OPDsmnfkRiHUnZ43octCMrlzA43ZNbPQw6HMgiZODwnizIt1G7ZFPusUey2O2C12ZDkierFMlD_GP0XUe_6_GHNeTu4NrgB3xZPOt0H_HV6bwoHq4u75fX5c3XL6vl4qY0c2hSiQ1rm5pK2XRdVwvKu7YW0vBu3TSatcBgjVTUTBqp5ZprIQAFrziniAD1nF8Ub4---XG_RoxJDTYa7Hvt0I9RVQ2v5nIOmciORBN8jAE7tQt2mldRUNPW1VZN2agpGwVM5Wyy6M3JfVwP2P6VnMLIhA9HAuYZ9xaDisZijqa1Ie9etd7-3__jP3LTW2eN7n_iAePWj1MyUVEVs0DdTT7T5wAJACIXvwE58bZr</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Ulusoy, Hasan B., MD, PhD</creator><creator>Gul, Husamettin, MD, PhD</creator><creator>Seyrek, Melik, MD, PhD</creator><creator>Yildiz, Oguzhan, MD, PhD</creator><creator>Ulku, Cunay, MD, PhD</creator><creator>Yıldırım, Vedat, MD</creator><creator>Kuralay, Erkan, MD</creator><creator>Celik, Turgay, MD</creator><creator>Yanarates, Omer, MD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080801</creationdate><title>The Concentration-Dependent Contractile Effect of Methylene Blue in the Human Internal Mammary Artery: A Quantitative Approach to Its Use in the Vasoplegic Syndrome</title><author>Ulusoy, Hasan B., MD, PhD ; Gul, Husamettin, MD, PhD ; Seyrek, Melik, MD, PhD ; Yildiz, Oguzhan, MD, PhD ; Ulku, Cunay, MD, PhD ; Yıldırım, Vedat, MD ; Kuralay, Erkan, MD ; Celik, Turgay, MD ; Yanarates, Omer, MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-e92d971889fff7513fd758c3fb99a2d020be15728c8a8b3a550e536331ee00743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Anesthesia & Perioperative Care</topic><topic>contraction</topic><topic>Critical Care</topic><topic>Dose-Response Relationship, Drug</topic><topic>human internal mammary artery</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Mammary Arteries - drug effects</topic><topic>Mammary Arteries - physiology</topic><topic>methylene blue</topic><topic>Methylene Blue - administration & dosage</topic><topic>Middle Aged</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Contraction - physiology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - physiology</topic><topic>Syndrome</topic><topic>Vasoconstriction - drug effects</topic><topic>Vasoconstriction - physiology</topic><topic>vasoplegic syndrome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ulusoy, Hasan B., MD, PhD</creatorcontrib><creatorcontrib>Gul, Husamettin, MD, PhD</creatorcontrib><creatorcontrib>Seyrek, Melik, MD, PhD</creatorcontrib><creatorcontrib>Yildiz, Oguzhan, MD, PhD</creatorcontrib><creatorcontrib>Ulku, Cunay, MD, PhD</creatorcontrib><creatorcontrib>Yıldırım, Vedat, MD</creatorcontrib><creatorcontrib>Kuralay, Erkan, MD</creatorcontrib><creatorcontrib>Celik, Turgay, MD</creatorcontrib><creatorcontrib>Yanarates, Omer, MD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cardiothoracic and vascular anesthesia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ulusoy, Hasan B., MD, PhD</au><au>Gul, Husamettin, MD, PhD</au><au>Seyrek, Melik, MD, PhD</au><au>Yildiz, Oguzhan, MD, PhD</au><au>Ulku, Cunay, MD, PhD</au><au>Yıldırım, Vedat, MD</au><au>Kuralay, Erkan, MD</au><au>Celik, Turgay, MD</au><au>Yanarates, Omer, MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Concentration-Dependent Contractile Effect of Methylene Blue in the Human Internal Mammary Artery: A Quantitative Approach to Its Use in the Vasoplegic Syndrome</atitle><jtitle>Journal of cardiothoracic and vascular anesthesia</jtitle><addtitle>J Cardiothorac Vasc Anesth</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>22</volume><issue>4</issue><spage>560</spage><epage>564</epage><pages>560-564</pages><issn>1053-0770</issn><eissn>1532-8422</eissn><abstract>Objective: To quantitate the contractile effect of methylene blue on isolated human internal mammary artery (IMA) as used in the vasoplegic syndrome. Design: An in vitro experimental study. Setting: Cardiovascular Pharmacology Laboratory, Department of Medical Pharmacology. Participants: IMA segments were used from 24 patients undergoing coronary artery bypass surgery. Interventions: The responses to methylene blue, norepinephrine, and acetylcholine were recorded isometrically by a force-displacement transducer in an isolated organ bath. Measurement and Main Results: Methylene blue (10 nmol/L-100 μmol/L) produced concentration-dependent contraction in the arteries. The maximal contraction to methylene blue was 44.2% ± 3.8% of KCl (68 mmol/L) maximum contraction; the pEC50 (−log10 of 50% effective concentration) value was 5.5 ± 0.1. Methylene blue caused an insignificant leftward shift of the concentration-response curve of norepinephrine. Acetylcholine-induced relaxation in submaximal contracted rings with phenylephrine recovered nearly 6 hours after the methylene blue challenge. Conclusion: Methylene blue caused concentration-dependent contraction in human IMAs. Furthermore, the inhibition of ACh-induced relaxation for 6 hours after the methylene blue challenge points out an additional mechanism (ie, receptor occupation). The concentration-dependent contractile effect of methylene blue justifies its use in the vasoplegic syndrome. The findings also suggest that the time course of contraction is longer than the exposure to methylene blue.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18662631</pmid><doi>10.1053/j.jvca.2008.02.018</doi><tpages>5</tpages></addata></record> |
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| subjects | Adult Aged Anesthesia & Perioperative Care contraction Critical Care Dose-Response Relationship, Drug human internal mammary artery Humans In Vitro Techniques Mammary Arteries - drug effects Mammary Arteries - physiology methylene blue Methylene Blue - administration & dosage Middle Aged Muscle Contraction - drug effects Muscle Contraction - physiology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - physiology Syndrome Vasoconstriction - drug effects Vasoconstriction - physiology vasoplegic syndrome |
| title | The Concentration-Dependent Contractile Effect of Methylene Blue in the Human Internal Mammary Artery: A Quantitative Approach to Its Use in the Vasoplegic Syndrome |
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