Highly efficient analysis of underivatized carbohydrates using monolithic-silica-based capillary hydrophilic interaction (HILIC) HPLC

A polyacrylamide (PAAm)-modified monolithic silica capillary column of increased phase ratio, 200T-PAAm, for hydrophilic interaction liquid chromatography (HILIC) was prepared. The column showed high separation efficiency, with a theoretical plate height H = 7-20 μm at a linear velocity, u = 1-7 mm/...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2008-08, Vol.391 (7), p.2533-2542
Hauptverfasser:	Ikegami, Tohru, Horie, Kanta, Saad, Nabil, Hosoya, Ken, Fiehn, Oliver, Tanaka, Nobuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylic Resins - chemistry Analytical Chemistry Biochemistry Carbohydrates - analysis Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography, High Pressure Liquid - methods Food Science HILIC HILIC-ESI-MS Kinetics Laboratory Medicine Monitoring/Environmental Analysis Monolithic silica Original Paper polyacrylamide Sensitivity and Specificity Silicon Dioxide - chemistry Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Underivatized carbohydrates
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