Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model
Abstract This study examined the vaccine potential of the autotransporter protein BrkA of Bordetella pertussis in the sublethal intranasal murine respiratory challenge model of infection. Five different acellular pertussis (Pa) vaccines, containing different pertussis-component antigens but all comp...
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| Veröffentlicht in: | Vaccine 2008-08, Vol.26 (34), p.4306-4311 |
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| creator | Marr, Nico Oliver, David C Laurent, Vincianne Poolman, Jan Denoël, Philippe Fernandez, Rachel C |
| description | Abstract This study examined the vaccine potential of the autotransporter protein BrkA of Bordetella pertussis in the sublethal intranasal murine respiratory challenge model of infection. Five different acellular pertussis (Pa) vaccines, containing different pertussis-component antigens but all comprizing diphtheria (D) and tetanus (T) toxoids, were tested. A two-pertussis-component DTPa vaccine containing pertussis toxoid (PT) and filamentous hemagglutinin (FHA) induced only limited bacterial clearance. However, a three-pertussis-component DTPa vaccine containing PT, FHA and a recombinant BrkA protein (rBrkA) was found to be as efficacious in protecting mice against colonization by B. pertussis strains Tohama I and 18-323 as the commercial Infanrix ™ vaccine that also includes PT and FHA but pertactin (PRN) instead of rBrkA. Vaccination of mice with rBrkA as the only B. pertussis antigen did not protect against colonization by B. pertussis . We also demonstrated that BrkA is ubiquitously expressed by highly prevalent clinical isolates of B. pertussis and suggest that new acellular pertussis vaccine formulations that include BrkA have equivalent efficacy as currently available DTPa vaccines against B. pertussis infections. |
| doi_str_mv | 10.1016/j.vaccine.2008.06.017 |
| format | Article |
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Five different acellular pertussis (Pa) vaccines, containing different pertussis-component antigens but all comprizing diphtheria (D) and tetanus (T) toxoids, were tested. A two-pertussis-component DTPa vaccine containing pertussis toxoid (PT) and filamentous hemagglutinin (FHA) induced only limited bacterial clearance. However, a three-pertussis-component DTPa vaccine containing PT, FHA and a recombinant BrkA protein (rBrkA) was found to be as efficacious in protecting mice against colonization by B. pertussis strains Tohama I and 18-323 as the commercial Infanrix ™ vaccine that also includes PT and FHA but pertactin (PRN) instead of rBrkA. Vaccination of mice with rBrkA as the only B. pertussis antigen did not protect against colonization by B. pertussis . We also demonstrated that BrkA is ubiquitously expressed by highly prevalent clinical isolates of B. pertussis and suggest that new acellular pertussis vaccine formulations that include BrkA have equivalent efficacy as currently available DTPa vaccines against B. pertussis infections.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2008.06.017</identifier><identifier>PMID: 18582518</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adhesins, Bacterial - immunology ; Allergy and Immunology ; Animals ; Applied microbiology ; Bacterial Outer Membrane Proteins - immunology ; Bacteriology ; Biological and medical sciences ; Bordetella pertussis ; Bordetella pertussis - growth & development ; Bordetella pertussis - immunology ; BrkA ; Colony Count, Microbial ; Diphtheria-Tetanus-acellular Pertussis Vaccines - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; Lung - immunology ; Lung - microbiology ; Mice ; Microbiology ; Miscellaneous ; Toxoids - immunology ; Vaccine ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Virulence Factors, Bordetella - immunology ; Whooping Cough - prevention & control</subject><ispartof>Vaccine, 2008-08, Vol.26 (34), p.4306-4311</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-8baf0f89f111b1c6a593030b760ddd9b03e7536ff50cd77a6f644469dac55fd93</citedby><cites>FETCH-LOGICAL-c479t-8baf0f89f111b1c6a593030b760ddd9b03e7536ff50cd77a6f644469dac55fd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X08007330$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20573830$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18582518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marr, Nico</creatorcontrib><creatorcontrib>Oliver, David C</creatorcontrib><creatorcontrib>Laurent, Vincianne</creatorcontrib><creatorcontrib>Poolman, Jan</creatorcontrib><creatorcontrib>Denoël, Philippe</creatorcontrib><creatorcontrib>Fernandez, Rachel C</creatorcontrib><title>Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract This study examined the vaccine potential of the autotransporter protein BrkA of Bordetella pertussis in the sublethal intranasal murine respiratory challenge model of infection. Five different acellular pertussis (Pa) vaccines, containing different pertussis-component antigens but all comprizing diphtheria (D) and tetanus (T) toxoids, were tested. A two-pertussis-component DTPa vaccine containing pertussis toxoid (PT) and filamentous hemagglutinin (FHA) induced only limited bacterial clearance. However, a three-pertussis-component DTPa vaccine containing PT, FHA and a recombinant BrkA protein (rBrkA) was found to be as efficacious in protecting mice against colonization by B. pertussis strains Tohama I and 18-323 as the commercial Infanrix ™ vaccine that also includes PT and FHA but pertactin (PRN) instead of rBrkA. Vaccination of mice with rBrkA as the only B. pertussis antigen did not protect against colonization by B. pertussis . We also demonstrated that BrkA is ubiquitously expressed by highly prevalent clinical isolates of B. pertussis and suggest that new acellular pertussis vaccine formulations that include BrkA have equivalent efficacy as currently available DTPa vaccines against B. pertussis infections.</description><subject>Adhesins, Bacterial - immunology</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Applied microbiology</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bordetella pertussis</subject><subject>Bordetella pertussis - growth & development</subject><subject>Bordetella pertussis - immunology</subject><subject>BrkA</subject><subject>Colony Count, Microbial</subject><subject>Diphtheria-Tetanus-acellular Pertussis Vaccines - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lung - immunology</subject><subject>Lung - microbiology</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Toxoids - immunology</subject><subject>Vaccine</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Virulence Factors, Bordetella - immunology</subject><subject>Whooping Cough - prevention & control</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9v1DAQxSMEokvhI4B8gVvCOE6c5AJqq_JHqgQSIHGzHHsMTpN4sZ2Vtp8eh41A4tLTXH4z8-a9ybLnFAoKlL8eioNUys5YlABtAbwA2jzIdrRtWF7WtH2Y7aDkVV5R-H6WPQlhAICa0e5xdkbbul2ZXTZ89i6iivaARK7FxiNxhsSfSC6d1xhxHCXZo49LCDaQS397QeQSXfRyDnvnI3pi5z8N0-KTIDIu8w-i3OhmeyejdTOZnMbxafbIyDHgs62eZ9_eXX-9-pDffHr_8eriJldV08W87aUB03aGUtpTxWXdMWDQNxy01l0PDJuacWNqULppJDe8qireaanq2uiOnWevTnP33v1aMEQx2aDWM2Z0SxC8Y5zSjt0LlhQ47VpIYH0ClXcheDRi7-0k_VFQEGsaYhBbGmJNQwAXKY3U92JbsPQT6n9dm_0JeLkBMig5mmSpsuEvV0LdsJatAt6eOEy-HSx6EZTFWaG2PoUntLP3Snnz3wQ12tmmpbd4xDC4xc8pFEFFKAWIL-vrrJ8DLUDDkoTfEjPBzA</recordid><startdate>20080812</startdate><enddate>20080812</enddate><creator>Marr, Nico</creator><creator>Oliver, David C</creator><creator>Laurent, Vincianne</creator><creator>Poolman, Jan</creator><creator>Denoël, Philippe</creator><creator>Fernandez, Rachel C</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080812</creationdate><title>Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model</title><author>Marr, Nico ; Oliver, David C ; Laurent, Vincianne ; Poolman, Jan ; Denoël, Philippe ; Fernandez, Rachel C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-8baf0f89f111b1c6a593030b760ddd9b03e7536ff50cd77a6f644469dac55fd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adhesins, Bacterial - immunology</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Applied microbiology</topic><topic>Bacterial Outer Membrane Proteins - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bordetella pertussis</topic><topic>Bordetella pertussis - growth & development</topic><topic>Bordetella pertussis - immunology</topic><topic>BrkA</topic><topic>Colony Count, Microbial</topic><topic>Diphtheria-Tetanus-acellular Pertussis Vaccines - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lung - immunology</topic><topic>Lung - microbiology</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Toxoids - immunology</topic><topic>Vaccine</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Virulence Factors, Bordetella - immunology</topic><topic>Whooping Cough - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marr, Nico</creatorcontrib><creatorcontrib>Oliver, David C</creatorcontrib><creatorcontrib>Laurent, Vincianne</creatorcontrib><creatorcontrib>Poolman, Jan</creatorcontrib><creatorcontrib>Denoël, Philippe</creatorcontrib><creatorcontrib>Fernandez, Rachel C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marr, Nico</au><au>Oliver, David C</au><au>Laurent, Vincianne</au><au>Poolman, Jan</au><au>Denoël, Philippe</au><au>Fernandez, Rachel C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2008-08-12</date><risdate>2008</risdate><volume>26</volume><issue>34</issue><spage>4306</spage><epage>4311</epage><pages>4306-4311</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract This study examined the vaccine potential of the autotransporter protein BrkA of Bordetella pertussis in the sublethal intranasal murine respiratory challenge model of infection. Five different acellular pertussis (Pa) vaccines, containing different pertussis-component antigens but all comprizing diphtheria (D) and tetanus (T) toxoids, were tested. A two-pertussis-component DTPa vaccine containing pertussis toxoid (PT) and filamentous hemagglutinin (FHA) induced only limited bacterial clearance. However, a three-pertussis-component DTPa vaccine containing PT, FHA and a recombinant BrkA protein (rBrkA) was found to be as efficacious in protecting mice against colonization by B. pertussis strains Tohama I and 18-323 as the commercial Infanrix ™ vaccine that also includes PT and FHA but pertactin (PRN) instead of rBrkA. Vaccination of mice with rBrkA as the only B. pertussis antigen did not protect against colonization by B. pertussis . We also demonstrated that BrkA is ubiquitously expressed by highly prevalent clinical isolates of B. pertussis and suggest that new acellular pertussis vaccine formulations that include BrkA have equivalent efficacy as currently available DTPa vaccines against B. pertussis infections.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18582518</pmid><doi>10.1016/j.vaccine.2008.06.017</doi><tpages>6</tpages></addata></record> |
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| subjects | Adhesins, Bacterial - immunology Allergy and Immunology Animals Applied microbiology Bacterial Outer Membrane Proteins - immunology Bacteriology Biological and medical sciences Bordetella pertussis Bordetella pertussis - growth & development Bordetella pertussis - immunology BrkA Colony Count, Microbial Diphtheria-Tetanus-acellular Pertussis Vaccines - immunology Female Fundamental and applied biological sciences. Psychology Lung - immunology Lung - microbiology Mice Microbiology Miscellaneous Toxoids - immunology Vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virulence Factors, Bordetella - immunology Whooping Cough - prevention & control |
| title | Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model |
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