Human rhinovirus HRV14 uncoats from early endosomes in the presence of bafilomycin

Determination of infectious progeny virus and in vivo labelling with [ 35S]methionine followed by immunoprecipitation demonstrates that the major receptor group human rhinovirus HRV14 is able to infect HeLa cells in the presence of the V-ATPase inhibitor bafilomycin A1. However, host cell shut off i...

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Veröffentlicht in:	FEBS letters 1999-12, Vol.463 (1), p.175-178
Hauptverfasser:	Bayer, Nora, Prchla, Elisabeth, Schwab, Martin, Blaas, Dieter, Fuchs, Renate
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Bafilomycin Capsid - metabolism Cytoplasm - metabolism Electrophoresis, Polyacrylamide Gel Endocytosis Endosomes - virology Enzyme Inhibitors - pharmacology HeLa Cells HRV, human rhinovirus Human rhinovirus Humans Hydrogen-Ion Concentration Macrolides MOI, multiplicity of infection p.i., post infection Precipitin Tests Proton-Translocating ATPases - antagonists & inhibitors Rhinovirus - drug effects Rhinovirus - metabolism RNA, Viral - metabolism TCID50, 50% tissue culture infectious dose Time Factors Viral uncoating
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