Aging Selectively Decreases Oxidative Capacity in Rat Heart Interfibrillar Mitochondria
Mitochondrial-derived oxidative injury contributes to cellular aging as well as to reperfusion-induced tissue damage. While the aging-heart suffers greater tissue damage following ischemia and reperfusion than the adult heart, the occurrence of aging-related alterations in mitochondrial oxidative me...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1999-12, Vol.372 (2), p.399-407 |
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| creator | Fannin, Stephen W. Lesnefsky, Edward J. Slabe, Thomas J. Hassan, Mehat O. Hoppel, Charles L. |
| description | Mitochondrial-derived oxidative injury contributes to cellular aging as well as to reperfusion-induced tissue damage. While the aging-heart suffers greater tissue damage following ischemia and reperfusion than the adult heart, the occurrence of aging-related alterations in mitochondrial oxidative metabolism in the elderly heart has remained uncertain. We determined if aging altered oxidative metabolism in either of the two populations of cardiac mitochondria, subsarcolemmal mitochondria (SSM) that reside beneath the plasma membrane or interfibrillar mitochondria (IFM) located between the myofibrils. SSM and IFM were isolated from 6-month adult and 24- and 28-month elderly Fischer 344 rat hearts. Aging-related alterations were limited to IFM, while SSM remained unaffected. Aging decreased the rate of oxidative phosphorylation in IFM, including when stimulated by electron donors specific for cytochrome oxidase. Cytochrome oxidase enzyme activity was decreased in IFM from aging hearts, while activity in SSM remained similar to adult controls. These findings allow future studies of aging-related decrements in oxidative function to focus upon IFM, while SSM provide an inherent control group of mitochondria that are free of aging-related alterations in oxidative function. The selective alteration of IFM during aging raises the possibility that the consequences of aging-induced mitochondrial dysfunction will be enhanced in specific subcellular regions of the senescent myocyte. |
| doi_str_mv | 10.1006/abbi.1999.1508 |
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While the aging-heart suffers greater tissue damage following ischemia and reperfusion than the adult heart, the occurrence of aging-related alterations in mitochondrial oxidative metabolism in the elderly heart has remained uncertain. We determined if aging altered oxidative metabolism in either of the two populations of cardiac mitochondria, subsarcolemmal mitochondria (SSM) that reside beneath the plasma membrane or interfibrillar mitochondria (IFM) located between the myofibrils. SSM and IFM were isolated from 6-month adult and 24- and 28-month elderly Fischer 344 rat hearts. Aging-related alterations were limited to IFM, while SSM remained unaffected. Aging decreased the rate of oxidative phosphorylation in IFM, including when stimulated by electron donors specific for cytochrome oxidase. Cytochrome oxidase enzyme activity was decreased in IFM from aging hearts, while activity in SSM remained similar to adult controls. These findings allow future studies of aging-related decrements in oxidative function to focus upon IFM, while SSM provide an inherent control group of mitochondria that are free of aging-related alterations in oxidative function. The selective alteration of IFM during aging raises the possibility that the consequences of aging-induced mitochondrial dysfunction will be enhanced in specific subcellular regions of the senescent myocyte.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1006/abbi.1999.1508</identifier><identifier>PMID: 10600182</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine Diphosphate - metabolism ; Aging - physiology ; Animals ; Ascorbic Acid - metabolism ; Cell Respiration - drug effects ; Citrate (si)-Synthase - metabolism ; cytochrome oxidase ; Electron Transport - drug effects ; Electron Transport Complex IV - metabolism ; Glutamic Acid - metabolism ; Male ; Mitochondria, Heart - enzymology ; Mitochondria, Heart - metabolism ; Mitochondria, Heart - ultrastructure ; Myocardium - cytology ; Myocardium - enzymology ; Myocardium - metabolism ; Myocardium - ultrastructure ; NADH Dehydrogenase - metabolism ; oxidative phosphorylation ; Oxidative Phosphorylation - drug effects ; Phosphatidylcholines ; Phospholipids - metabolism ; Rats ; Rats, Inbred F344 ; Sarcolemma - enzymology ; Sarcolemma - metabolism ; Sarcolemma - ultrastructure ; Succinate Cytochrome c Oxidoreductase - metabolism ; Uncoupling Agents - pharmacology</subject><ispartof>Archives of biochemistry and biophysics, 1999-12, Vol.372 (2), p.399-407</ispartof><rights>1999 Academic Press</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-50f39afcba836a58a17e5ba475364be39e574d36b270cf06ac66937ec223a66a3</citedby><cites>FETCH-LOGICAL-c406t-50f39afcba836a58a17e5ba475364be39e574d36b270cf06ac66937ec223a66a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/abbi.1999.1508$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10600182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fannin, Stephen W.</creatorcontrib><creatorcontrib>Lesnefsky, Edward J.</creatorcontrib><creatorcontrib>Slabe, Thomas J.</creatorcontrib><creatorcontrib>Hassan, Mehat O.</creatorcontrib><creatorcontrib>Hoppel, Charles L.</creatorcontrib><title>Aging Selectively Decreases Oxidative Capacity in Rat Heart Interfibrillar Mitochondria</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Mitochondrial-derived oxidative injury contributes to cellular aging as well as to reperfusion-induced tissue damage. While the aging-heart suffers greater tissue damage following ischemia and reperfusion than the adult heart, the occurrence of aging-related alterations in mitochondrial oxidative metabolism in the elderly heart has remained uncertain. We determined if aging altered oxidative metabolism in either of the two populations of cardiac mitochondria, subsarcolemmal mitochondria (SSM) that reside beneath the plasma membrane or interfibrillar mitochondria (IFM) located between the myofibrils. SSM and IFM were isolated from 6-month adult and 24- and 28-month elderly Fischer 344 rat hearts. Aging-related alterations were limited to IFM, while SSM remained unaffected. Aging decreased the rate of oxidative phosphorylation in IFM, including when stimulated by electron donors specific for cytochrome oxidase. Cytochrome oxidase enzyme activity was decreased in IFM from aging hearts, while activity in SSM remained similar to adult controls. These findings allow future studies of aging-related decrements in oxidative function to focus upon IFM, while SSM provide an inherent control group of mitochondria that are free of aging-related alterations in oxidative function. The selective alteration of IFM during aging raises the possibility that the consequences of aging-induced mitochondrial dysfunction will be enhanced in specific subcellular regions of the senescent myocyte.</description><subject>Adenosine Diphosphate - metabolism</subject><subject>Aging - physiology</subject><subject>Animals</subject><subject>Ascorbic Acid - metabolism</subject><subject>Cell Respiration - drug effects</subject><subject>Citrate (si)-Synthase - metabolism</subject><subject>cytochrome oxidase</subject><subject>Electron Transport - drug effects</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Glutamic Acid - metabolism</subject><subject>Male</subject><subject>Mitochondria, Heart - enzymology</subject><subject>Mitochondria, Heart - metabolism</subject><subject>Mitochondria, Heart - ultrastructure</subject><subject>Myocardium - cytology</subject><subject>Myocardium - enzymology</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - ultrastructure</subject><subject>NADH Dehydrogenase - metabolism</subject><subject>oxidative phosphorylation</subject><subject>Oxidative Phosphorylation - drug effects</subject><subject>Phosphatidylcholines</subject><subject>Phospholipids - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Sarcolemma - enzymology</subject><subject>Sarcolemma - metabolism</subject><subject>Sarcolemma - ultrastructure</subject><subject>Succinate Cytochrome c Oxidoreductase - metabolism</subject><subject>Uncoupling Agents - pharmacology</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1PwzAMhiMEgjG4ckQ5cetwmjZtjtP4lIYm8SGOkZu6I6hrR9JN7N_Tahy4cLJsPX5lP4xdCJgIAHWNReEmQms9ESnkB2wkQKsIZJ4cshEAyEjnSpyw0xA-AYRIVHzMTgSovsnjEXufLl2z5C9Uk-3cluodvyHrCQMFvvh2JQ5TPsM1WtftuGv4M3b8gdB3_LHpyFeu8K6u0fMn17X2o21K7_CMHVVYBzr_rWP2dnf7OnuI5ov7x9l0HtkEVBelUEmNlS0wlwrTHEVGaYFJlkqVFCQ1pVlSSlXEGdgKFFqltMzIxrFEpVCO2dU-d-3brw2FzqxcsNTf01C7CaanFcSge3CyB61vQ_BUmbV3K_Q7I8AMKs2g0gwqzaCyX7j8Td4UKyr_4Ht3PZDvAer_2zryJlhHjaXS-V6mKVv3X_YPlx-C5Q</recordid><startdate>19991215</startdate><enddate>19991215</enddate><creator>Fannin, Stephen W.</creator><creator>Lesnefsky, Edward J.</creator><creator>Slabe, Thomas J.</creator><creator>Hassan, Mehat O.</creator><creator>Hoppel, Charles L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991215</creationdate><title>Aging Selectively Decreases Oxidative Capacity in Rat Heart Interfibrillar Mitochondria</title><author>Fannin, Stephen W. ; Lesnefsky, Edward J. ; Slabe, Thomas J. ; Hassan, Mehat O. ; Hoppel, Charles L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-50f39afcba836a58a17e5ba475364be39e574d36b270cf06ac66937ec223a66a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenosine Diphosphate - metabolism</topic><topic>Aging - physiology</topic><topic>Animals</topic><topic>Ascorbic Acid - metabolism</topic><topic>Cell Respiration - drug effects</topic><topic>Citrate (si)-Synthase - metabolism</topic><topic>cytochrome oxidase</topic><topic>Electron Transport - drug effects</topic><topic>Electron Transport Complex IV - metabolism</topic><topic>Glutamic Acid - metabolism</topic><topic>Male</topic><topic>Mitochondria, Heart - enzymology</topic><topic>Mitochondria, Heart - metabolism</topic><topic>Mitochondria, Heart - ultrastructure</topic><topic>Myocardium - cytology</topic><topic>Myocardium - enzymology</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - ultrastructure</topic><topic>NADH Dehydrogenase - metabolism</topic><topic>oxidative phosphorylation</topic><topic>Oxidative Phosphorylation - drug effects</topic><topic>Phosphatidylcholines</topic><topic>Phospholipids - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Sarcolemma - enzymology</topic><topic>Sarcolemma - metabolism</topic><topic>Sarcolemma - ultrastructure</topic><topic>Succinate Cytochrome c Oxidoreductase - metabolism</topic><topic>Uncoupling Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fannin, Stephen W.</creatorcontrib><creatorcontrib>Lesnefsky, Edward J.</creatorcontrib><creatorcontrib>Slabe, Thomas J.</creatorcontrib><creatorcontrib>Hassan, Mehat O.</creatorcontrib><creatorcontrib>Hoppel, Charles L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fannin, Stephen W.</au><au>Lesnefsky, Edward J.</au><au>Slabe, Thomas J.</au><au>Hassan, Mehat O.</au><au>Hoppel, Charles L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aging Selectively Decreases Oxidative Capacity in Rat Heart Interfibrillar Mitochondria</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1999-12-15</date><risdate>1999</risdate><volume>372</volume><issue>2</issue><spage>399</spage><epage>407</epage><pages>399-407</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>Mitochondrial-derived oxidative injury contributes to cellular aging as well as to reperfusion-induced tissue damage. While the aging-heart suffers greater tissue damage following ischemia and reperfusion than the adult heart, the occurrence of aging-related alterations in mitochondrial oxidative metabolism in the elderly heart has remained uncertain. We determined if aging altered oxidative metabolism in either of the two populations of cardiac mitochondria, subsarcolemmal mitochondria (SSM) that reside beneath the plasma membrane or interfibrillar mitochondria (IFM) located between the myofibrils. SSM and IFM were isolated from 6-month adult and 24- and 28-month elderly Fischer 344 rat hearts. Aging-related alterations were limited to IFM, while SSM remained unaffected. Aging decreased the rate of oxidative phosphorylation in IFM, including when stimulated by electron donors specific for cytochrome oxidase. Cytochrome oxidase enzyme activity was decreased in IFM from aging hearts, while activity in SSM remained similar to adult controls. These findings allow future studies of aging-related decrements in oxidative function to focus upon IFM, while SSM provide an inherent control group of mitochondria that are free of aging-related alterations in oxidative function. The selective alteration of IFM during aging raises the possibility that the consequences of aging-induced mitochondrial dysfunction will be enhanced in specific subcellular regions of the senescent myocyte.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10600182</pmid><doi>10.1006/abbi.1999.1508</doi><tpages>9</tpages></addata></record> |
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| subjects | Adenosine Diphosphate - metabolism Aging - physiology Animals Ascorbic Acid - metabolism Cell Respiration - drug effects Citrate (si)-Synthase - metabolism cytochrome oxidase Electron Transport - drug effects Electron Transport Complex IV - metabolism Glutamic Acid - metabolism Male Mitochondria, Heart - enzymology Mitochondria, Heart - metabolism Mitochondria, Heart - ultrastructure Myocardium - cytology Myocardium - enzymology Myocardium - metabolism Myocardium - ultrastructure NADH Dehydrogenase - metabolism oxidative phosphorylation Oxidative Phosphorylation - drug effects Phosphatidylcholines Phospholipids - metabolism Rats Rats, Inbred F344 Sarcolemma - enzymology Sarcolemma - metabolism Sarcolemma - ultrastructure Succinate Cytochrome c Oxidoreductase - metabolism Uncoupling Agents - pharmacology |
| title | Aging Selectively Decreases Oxidative Capacity in Rat Heart Interfibrillar Mitochondria |
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