A versatile microporation technique for the transfection of cultured CNS neurons

The application of molecular techniques to cultured central nervous system (CNS) neurons has been limited by a lack of simple and efficient methods to introduce macromolecules into their cytosol. We have developed an electroporation technique that efficiently transfers RNA, DNA and other large membr...

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Veröffentlicht in:	Journal of neuroscience methods 1999-10, Vol.93 (1), p.37-48
Hauptverfasser:	Teruel, Mary N, Blanpied, Thomas A, Shen, Kang, Augustine, George J, Meyer, Tobias
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Newborn Biological and medical sciences Cells, Cultured CFP DNA, Complementary - genetics Electroporation Electroporation - instrumentation Electroporation - methods Fundamental and applied biological sciences. Psychology General aspects. Models. Methods GFP Hippocampal neurons Hippocampus - physiology Neurons - physiology Quantitative co-transfection Rats Rats, Sprague-Dawley RNA RNA - genetics Transfection - instrumentation Transfection - methods Vertebrates: nervous system and sense organs YFP
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