Purification and characterization of agarases from a marine bacterium Vibrio sp. F-6

Marine bacterium Vibrio sp. F-6, utilizing agarose as a carbon source to produce agarases, was isolated from seawater samples taken from Qingdao, China. Two agarases (AG-a and AG-b) were purified to a homogeneity from the cultural supernatant of Vibrio sp. F-6 through ammonium sulfate precipitation,...

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Veröffentlicht in:	Journal of industrial microbiology & biotechnology 2008-08, Vol.35 (8), p.915-922
Hauptverfasser:	Fu, Wandong, Han, Baoqin, Duan, Delin, Liu, Wanshun, Wang, Changhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Ammonium Sulfate Bacteria Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Biochemistry Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biotechnology Carbon - metabolism Carbon sources Cations, Divalent - pharmacology Chemical analysis Chemical Fractionation China Chromatography Chromatography, Gel Chromatography, Ion Exchange Culture Media - chemistry Edetic Acid - pharmacology Electrophoresis, Polyacrylamide Gel Enzyme Activators - pharmacology Enzyme Inhibitors - pharmacology Enzyme Stability Enzymes Fundamental and applied biological sciences. Psychology Galactosides - metabolism Genetic Engineering Glycoside Hydrolases - chemistry Glycoside Hydrolases - isolation & purification Glycoside Hydrolases - metabolism Hydrogen-Ion Concentration Inorganic Chemistry Life Sciences Marine biology Metals - pharmacology Microbiology Molecular Weight Oligosaccharides - metabolism Original Paper Phylogeny Proteins RNA, Ribosomal, 16S - genetics Seawater Seawater - microbiology Sepharose - metabolism Sequence Analysis, DNA Sodium Dodecyl Sulfate - pharmacology Studies Sulfates Temperature Vibrio - classification Vibrio - enzymology Vibrio - isolation & purification Water analysis
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