Reactions of Type II Restriction Endonucleases with 8-Base Pair Recognition Sites

Type II restriction endonucleases usually recognize 4â6-base pair (bp) sites on DNA and cleave each site in a separate reaction. A few type II endonucleases have 8-bp recognition sites, but these seem unsuited for restriction, since their sites are rare on most DNA. Moreover, only one endonuclease...

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Veröffentlicht in:	The Journal of biological chemistry 1999-12, Vol.274 (51), p.36379-36386
Hauptverfasser:	Bilcock, D T, Daniels, L E, Bath, A J, Halford, S E
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Pairing Binding Sites - genetics deoxyribonuclease FseI deoxyribonuclease PacI deoxyribonuclease PmeI deoxyribonuclease SfiI deoxyribonuclease SgfI deoxyribonuclease SrfI Deoxyribonucleases, Type II Site-Specific - chemistry Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - metabolism DNA - genetics DNA - metabolism Plasmids Sequence Analysis Substrate Specificity
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