Reactions of Type II Restriction Endonucleases with 8-Base Pair Recognition Sites
Type II restriction endonucleases usually recognize 4â6-base pair (bp) sites on DNA and cleave each site in a separate reaction. A few type II endonucleases have 8-bp recognition sites, but these seem unsuited for restriction, since their sites are rare on most DNA. Moreover, only one endonuclease...
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Veröffentlicht in: | The Journal of biological chemistry 1999-12, Vol.274 (51), p.36379-36386 |
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container_title | The Journal of biological chemistry |
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creator | Bilcock, D T Daniels, L E Bath, A J Halford, S E |
description | Type II restriction endonucleases usually recognize 4â6-base pair (bp) sites on DNA and cleave each site in a separate reaction.
A few type II endonucleases have 8-bp recognition sites, but these seem unsuited for restriction, since their sites are rare
on most DNA. Moreover, only one endonuclease that recognizes a target containing 8 bp has been examined to date, and this
enzyme, Sfi I, needs two copies of this site for its DNA cleavage reaction. In this study, several endonucleases with 8-bp sites were
tested on plasmids that have either one or two copies of the relevant sequence to determine if they also need two sites. Sgf I, Srf I, Fse I, Pac I, Pme I, Sse 8781I, and Sda I all acted through equal and independent reactions at each site. Asc I cleaved the DNA with one site at the same rate as that with two sites but acted processively on the latter. In contrast,
Sgr AI showed a marked preference for the plasmid with two sites and cleaved both sites on this DNA in a concerted manner, like
Sfi I. Endonucleases that require two copies of an 8-bp sequence may be widespread in nature, where, despite this seemingly inappropriate
requirement, they may function in DNA restriction. |
doi_str_mv | 10.1074/jbc.274.51.36379 |
format | Article |
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A few type II endonucleases have 8-bp recognition sites, but these seem unsuited for restriction, since their sites are rare
on most DNA. Moreover, only one endonuclease that recognizes a target containing 8 bp has been examined to date, and this
enzyme, Sfi I, needs two copies of this site for its DNA cleavage reaction. In this study, several endonucleases with 8-bp sites were
tested on plasmids that have either one or two copies of the relevant sequence to determine if they also need two sites. Sgf I, Srf I, Fse I, Pac I, Pme I, Sse 8781I, and Sda I all acted through equal and independent reactions at each site. Asc I cleaved the DNA with one site at the same rate as that with two sites but acted processively on the latter. In contrast,
Sgr AI showed a marked preference for the plasmid with two sites and cleaved both sites on this DNA in a concerted manner, like
Sfi I. Endonucleases that require two copies of an 8-bp sequence may be widespread in nature, where, despite this seemingly inappropriate
requirement, they may function in DNA restriction.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.274.51.36379</identifier><identifier>PMID: 10593932</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Base Pairing ; Binding Sites - genetics ; deoxyribonuclease FseI ; deoxyribonuclease PacI ; deoxyribonuclease PmeI ; deoxyribonuclease SfiI ; deoxyribonuclease SgfI ; deoxyribonuclease SrfI ; Deoxyribonucleases, Type II Site-Specific - chemistry ; Deoxyribonucleases, Type II Site-Specific - genetics ; Deoxyribonucleases, Type II Site-Specific - metabolism ; DNA - genetics ; DNA - metabolism ; Plasmids ; Sequence Analysis ; Substrate Specificity</subject><ispartof>The Journal of biological chemistry, 1999-12, Vol.274 (51), p.36379-36386</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-592e062de1eac39c9a5859b1edebcd676cde0b5a423b8d0d84641e5676d187193</citedby><cites>FETCH-LOGICAL-c397t-592e062de1eac39c9a5859b1edebcd676cde0b5a423b8d0d84641e5676d187193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10593932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bilcock, D T</creatorcontrib><creatorcontrib>Daniels, L E</creatorcontrib><creatorcontrib>Bath, A J</creatorcontrib><creatorcontrib>Halford, S E</creatorcontrib><title>Reactions of Type II Restriction Endonucleases with 8-Base Pair Recognition Sites</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Type II restriction endonucleases usually recognize 4â6-base pair (bp) sites on DNA and cleave each site in a separate reaction.
A few type II endonucleases have 8-bp recognition sites, but these seem unsuited for restriction, since their sites are rare
on most DNA. Moreover, only one endonuclease that recognizes a target containing 8 bp has been examined to date, and this
enzyme, Sfi I, needs two copies of this site for its DNA cleavage reaction. In this study, several endonucleases with 8-bp sites were
tested on plasmids that have either one or two copies of the relevant sequence to determine if they also need two sites. Sgf I, Srf I, Fse I, Pac I, Pme I, Sse 8781I, and Sda I all acted through equal and independent reactions at each site. Asc I cleaved the DNA with one site at the same rate as that with two sites but acted processively on the latter. In contrast,
Sgr AI showed a marked preference for the plasmid with two sites and cleaved both sites on this DNA in a concerted manner, like
Sfi I. Endonucleases that require two copies of an 8-bp sequence may be widespread in nature, where, despite this seemingly inappropriate
requirement, they may function in DNA restriction.</description><subject>Base Pairing</subject><subject>Binding Sites - genetics</subject><subject>deoxyribonuclease FseI</subject><subject>deoxyribonuclease PacI</subject><subject>deoxyribonuclease PmeI</subject><subject>deoxyribonuclease SfiI</subject><subject>deoxyribonuclease SgfI</subject><subject>deoxyribonuclease SrfI</subject><subject>Deoxyribonucleases, Type II Site-Specific - chemistry</subject><subject>Deoxyribonucleases, Type II Site-Specific - genetics</subject><subject>Deoxyribonucleases, Type II Site-Specific - metabolism</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>Plasmids</subject><subject>Sequence Analysis</subject><subject>Substrate Specificity</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDFPwzAQRi0EoqWwM6EMiC3BjuM4HqEqUAkJKEVisxL72rhK4xInqvrvMU0HmPBy8t27T6eH0CXBEcE8uV0VKop5EjES0ZRycYSGBGc0pIx8HqMhxjEJRcyyATpzboX9SwQ5RQOCmaCCxkP0NoNctcbWLrCLYL7bQDCdBjNwbWP2_WBSa1t3qoLcgQu2pi2DLLz3n-A1N41HlV3WZo--mxbcOTpZ5JWDi0MdoY-HyXz8FD6_PE7Hd8-hooK3IRMx4DTWQPwBVCiRs4yJgoCGQumUp0oDLliexLTINNZZkiYEmB9oknEi6Ajd9Lmbxn51_mC5Nk5BVeU12M7JVFBGaUz_BQlPGKOMexD3oGqscw0s5KYx67zZSYLlj2_pfUvvWzIi9779ytUhuyvWoH8t9II9cN0DpVmWW9OALIxVJaz_5nwDDaKHEg</recordid><startdate>19991217</startdate><enddate>19991217</enddate><creator>Bilcock, D T</creator><creator>Daniels, L E</creator><creator>Bath, A J</creator><creator>Halford, S E</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19991217</creationdate><title>Reactions of Type II Restriction Endonucleases with 8-Base Pair Recognition Sites</title><author>Bilcock, D T ; Daniels, L E ; Bath, A J ; Halford, S E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-592e062de1eac39c9a5859b1edebcd676cde0b5a423b8d0d84641e5676d187193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Base Pairing</topic><topic>Binding Sites - genetics</topic><topic>deoxyribonuclease FseI</topic><topic>deoxyribonuclease PacI</topic><topic>deoxyribonuclease PmeI</topic><topic>deoxyribonuclease SfiI</topic><topic>deoxyribonuclease SgfI</topic><topic>deoxyribonuclease SrfI</topic><topic>Deoxyribonucleases, Type II Site-Specific - chemistry</topic><topic>Deoxyribonucleases, Type II Site-Specific - genetics</topic><topic>Deoxyribonucleases, Type II Site-Specific - metabolism</topic><topic>DNA - genetics</topic><topic>DNA - metabolism</topic><topic>Plasmids</topic><topic>Sequence Analysis</topic><topic>Substrate Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bilcock, D T</creatorcontrib><creatorcontrib>Daniels, L E</creatorcontrib><creatorcontrib>Bath, A J</creatorcontrib><creatorcontrib>Halford, S E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bilcock, D T</au><au>Daniels, L E</au><au>Bath, A J</au><au>Halford, S E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reactions of Type II Restriction Endonucleases with 8-Base Pair Recognition Sites</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1999-12-17</date><risdate>1999</risdate><volume>274</volume><issue>51</issue><spage>36379</spage><epage>36386</epage><pages>36379-36386</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Type II restriction endonucleases usually recognize 4â6-base pair (bp) sites on DNA and cleave each site in a separate reaction.
A few type II endonucleases have 8-bp recognition sites, but these seem unsuited for restriction, since their sites are rare
on most DNA. Moreover, only one endonuclease that recognizes a target containing 8 bp has been examined to date, and this
enzyme, Sfi I, needs two copies of this site for its DNA cleavage reaction. In this study, several endonucleases with 8-bp sites were
tested on plasmids that have either one or two copies of the relevant sequence to determine if they also need two sites. Sgf I, Srf I, Fse I, Pac I, Pme I, Sse 8781I, and Sda I all acted through equal and independent reactions at each site. Asc I cleaved the DNA with one site at the same rate as that with two sites but acted processively on the latter. In contrast,
Sgr AI showed a marked preference for the plasmid with two sites and cleaved both sites on this DNA in a concerted manner, like
Sfi I. Endonucleases that require two copies of an 8-bp sequence may be widespread in nature, where, despite this seemingly inappropriate
requirement, they may function in DNA restriction.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>10593932</pmid><doi>10.1074/jbc.274.51.36379</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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language | eng |
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source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
subjects | Base Pairing Binding Sites - genetics deoxyribonuclease FseI deoxyribonuclease PacI deoxyribonuclease PmeI deoxyribonuclease SfiI deoxyribonuclease SgfI deoxyribonuclease SrfI Deoxyribonucleases, Type II Site-Specific - chemistry Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - metabolism DNA - genetics DNA - metabolism Plasmids Sequence Analysis Substrate Specificity |
title | Reactions of Type II Restriction Endonucleases with 8-Base Pair Recognition Sites |
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