Radial Capillary Array Electrophoresis Microplate and Scanner for High-Performance Nucleic Acid Analysis
The design, fabrication, and operation of a radial capillary array electrophoresis microplate and scanner for high-throughput DNA analysis is presented. The microplate consists of a central common anode reservoir coupled to 96 separate microfabricated separation channels connected to sample injector...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1999-12, Vol.71 (23), p.5354-5361 |
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| container_issue | 23 |
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| container_title | Analytical chemistry (Washington) |
| container_volume | 71 |
| creator | Shi, Yining Simpson, Peter C Scherer, James R Wexler, David Skibola, Christine Smith, Martyn T Mathies, Richard A |
| description | The design, fabrication, and operation of a radial capillary array electrophoresis microplate and scanner for high-throughput DNA analysis is presented. The microplate consists of a central common anode reservoir coupled to 96 separate microfabricated separation channels connected to sample injectors on the perimeter of the 10-cm-diameter wafer. Detection is accomplished by a laser-excited rotary confocal scanner with four color detection channels. Loading of 96 samples in parallel is achieved using a pressurized capillary array system. High-quality separations of 96 pBR322 restriction digest samples are achieved in |
| doi_str_mv | 10.1021/ac990518p |
| format | Article |
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The microplate consists of a central common anode reservoir coupled to 96 separate microfabricated separation channels connected to sample injectors on the perimeter of the 10-cm-diameter wafer. Detection is accomplished by a laser-excited rotary confocal scanner with four color detection channels. Loading of 96 samples in parallel is achieved using a pressurized capillary array system. High-quality separations of 96 pBR322 restriction digest samples are achieved in <120 s with the microplate system. The practical utility and multicolor detection capability is demonstrated by analyzing 96 methylenetetrahydrofolate reductase (MTHFR) alleles in parallel using a noncovalent 2-color staining method. This work establishes the feasibility of performing high-throughput genotyping separations with capillary array electrophoresis microplates.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac990518p</identifier><identifier>PMID: 10596215</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Acids ; Analysis ; Biological and medical sciences ; Blood vessels ; Deoxyribonucleic acid ; Diverse techniques ; DNA ; Electrophoresis, Capillary - methods ; Fundamental and applied biological sciences. 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| ispartof | Analytical chemistry (Washington), 1999-12, Vol.71 (23), p.5354-5361 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Acids Analysis Biological and medical sciences Blood vessels Deoxyribonucleic acid Diverse techniques DNA Electrophoresis, Capillary - methods Fundamental and applied biological sciences. Psychology Molecular and cellular biology Nucleic Acids - analysis |
| title | Radial Capillary Array Electrophoresis Microplate and Scanner for High-Performance Nucleic Acid Analysis |
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