Microwave-stimulated recovery of myosin-V immunoreactivity from formalin-fixed, paraffin-embedded human CNS

The lability of brain myosin-V (BM-V) to aldehyde-fixation has hindered immunohistochemical (IH) studies of this actin-based motor. We show here that BM-V immunoreactivity (IR) can be retrieved from formalin-fixed, paraffin-embedded human tissue. BM-V IR was optimally retrieved by boiling 5 μm cereb...

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Veröffentlicht in:	Journal of neuroscience methods 1999-10, Vol.92 (1), p.25-29
Hauptverfasser:	Martins, Antonio R., Dias, Minna M., Vasconcelos, Thaisa M., Caldo, Hildeberto, Costa, Maria C.R., Chimelli, Leila, Larson, Roy E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Aged, 80 and over Animals Antigen retrieval Biological and medical sciences Calmodulin-Binding Proteins - isolation & purification Cerebellum Cerebellum - chemistry Chickens Child, Preschool Fetus Formaldehyde Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Granule cell Human nervous system Humans Immunohistochemistry Immunohistochemistry - methods Infant Microwave Microwaves Myosin Type V Myosin-V Nerve Tissue Proteins - isolation & purification Paraffin Embedding Purkinje cell Rabbits Temporal Lobe - chemistry Vertebrates: nervous system and sense organs
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