Fast scanning and efficient photodetection in a simple two-photon microscope

Two-photon laser scan microscopy carries many advantages for work on brain slices and bulk tissue. However, it has very low signal levels compared to conventional fluorescence microscopy. This is disadvantageous in fast imaging applications when photon shot noise is limiting. Working on brain slices...

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Veröffentlicht in:	Journal of neuroscience methods 1999-10, Vol.92 (1), p.123-135
Hauptverfasser:	Tan, Y.P, Llano, I, Hopt, A, Würriehausen, F, Neher, E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Calcium signaling Cerebellum - ultrastructure Fluorescence Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Microscopy, Confocal - methods Microscopy, Fluorescence - methods Non-linear optics Photodetection Photons Purkinje Cells - ultrastructure Scanning Vertebrates: nervous system and sense organs
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