Harpin induces mitogen-activated protein kinase activity during defence responses in Arabidopsis thaliana suspension cultures
Elicitation of Arabidopsis thaliana (L.) Heynh. suspension cultures with the bacterial protein harpin (from Pseudomonas syringae pv. syringae) induced the activation of two kinases of 39 and 44 kDa, as demonstrated by in-gel kinase assays using myelin basic protein (MBP) as a substrate. Both these k...
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| Veröffentlicht in: | Planta 1999-11, Vol.210 (1), p.97-103 |
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| creator | Desikan, R Clarke, A Atherfold, P Hancock, J.T Neill, S.J |
| description | Elicitation of Arabidopsis thaliana (L.) Heynh. suspension cultures with the bacterial protein harpin (from Pseudomonas syringae pv. syringae) induced the activation of two kinases of 39 and 44 kDa, as demonstrated by in-gel kinase assays using myelin basic protein (MBP) as a substrate. Both these kinases appeared to be tyrosine-phosphorylated upon activation, as demonstrated by treatment with tyrosine phosphatase and immunoprecipitation using an anti-phosphotyrosine monoclonal antibody. An inhibitor of mammalian mitogen-activated protein kinase (MAPK) activation, PD98059, inhibited harpin-induced MBPK activation, but did not inhibit the activity of these kinases. PD98059 also inhibited harpin-induced programmed cell death and defence gene expression, suggesting the involvement of harpin-induced MAPKs in defence responses in Arabidopsis thaliana. |
| doi_str_mv | 10.1007/s004250050658 |
| format | Article |
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Heynh. suspension cultures with the bacterial protein harpin (from Pseudomonas syringae pv. syringae) induced the activation of two kinases of 39 and 44 kDa, as demonstrated by in-gel kinase assays using myelin basic protein (MBP) as a substrate. Both these kinases appeared to be tyrosine-phosphorylated upon activation, as demonstrated by treatment with tyrosine phosphatase and immunoprecipitation using an anti-phosphotyrosine monoclonal antibody. An inhibitor of mammalian mitogen-activated protein kinase (MAPK) activation, PD98059, inhibited harpin-induced MBPK activation, but did not inhibit the activity of these kinases. PD98059 also inhibited harpin-induced programmed cell death and defence gene expression, suggesting the involvement of harpin-induced MAPKs in defence responses in Arabidopsis thaliana.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s004250050658</identifier><identifier>PMID: 10592037</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Animal and plant ecology ; Animal, plant and microbial ecology ; Antibodies ; Apoptosis ; Apoptosis - drug effects ; Arabidopsis - cytology ; Arabidopsis - enzymology ; Arabidopsis thaliana ; Autoecology ; Bacterial Outer Membrane Proteins - pharmacology ; bacterial proteins ; Biological and medical sciences ; cell suspension culture ; Cells, Cultured ; defense mechanisms ; Enzyme Activation ; enzyme activity ; Enzyme Induction ; enzyme inhibitors ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gels ; Gene expression ; Generalities. Disease free stocks ; Hypersensitive response ; immunochemistry ; Mitogen-Activated Protein Kinases - antagonists & inhibitors ; Mitogen-Activated Protein Kinases - biosynthesis ; Mitogen-Activated Protein Kinases - metabolism ; mitogens ; molecular weight ; Phosphatases ; Phosphorylation ; Phytopathology. Animal pests. Plant and forest protection ; Plant cells ; plant proteins ; Plants ; Plants and fungi ; Pseudomonas syringae pv. syringae ; RNA ; substrates ; tyrosine ; Tyrosine - metabolism</subject><ispartof>Planta, 1999-11, Vol.210 (1), p.97-103</ispartof><rights>Springer-Verlag Berlin Heidelberg 1999</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-9ae7ee73dcf7f08d94bd1de5464b77525a5dce3fb9b05ad16cd1aa17e142c4e63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23385847$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23385847$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=933318$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10592037$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Desikan, R</creatorcontrib><creatorcontrib>Clarke, A</creatorcontrib><creatorcontrib>Atherfold, P</creatorcontrib><creatorcontrib>Hancock, J.T</creatorcontrib><creatorcontrib>Neill, S.J</creatorcontrib><title>Harpin induces mitogen-activated protein kinase activity during defence responses in Arabidopsis thaliana suspension cultures</title><title>Planta</title><addtitle>Planta</addtitle><description>Elicitation of Arabidopsis thaliana (L.) Heynh. suspension cultures with the bacterial protein harpin (from Pseudomonas syringae pv. syringae) induced the activation of two kinases of 39 and 44 kDa, as demonstrated by in-gel kinase assays using myelin basic protein (MBP) as a substrate. Both these kinases appeared to be tyrosine-phosphorylated upon activation, as demonstrated by treatment with tyrosine phosphatase and immunoprecipitation using an anti-phosphotyrosine monoclonal antibody. An inhibitor of mammalian mitogen-activated protein kinase (MAPK) activation, PD98059, inhibited harpin-induced MBPK activation, but did not inhibit the activity of these kinases. PD98059 also inhibited harpin-induced programmed cell death and defence gene expression, suggesting the involvement of harpin-induced MAPKs in defence responses in Arabidopsis thaliana.</description><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Antibodies</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis thaliana</subject><subject>Autoecology</subject><subject>Bacterial Outer Membrane Proteins - pharmacology</subject><subject>bacterial proteins</subject><subject>Biological and medical sciences</subject><subject>cell suspension culture</subject><subject>Cells, Cultured</subject><subject>defense mechanisms</subject><subject>Enzyme Activation</subject><subject>enzyme activity</subject><subject>Enzyme Induction</subject><subject>enzyme inhibitors</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Gene expression</subject><subject>Generalities. Disease free stocks</subject><subject>Hypersensitive response</subject><subject>immunochemistry</subject><subject>Mitogen-Activated Protein Kinases - antagonists & inhibitors</subject><subject>Mitogen-Activated Protein Kinases - biosynthesis</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>mitogens</subject><subject>molecular weight</subject><subject>Phosphatases</subject><subject>Phosphorylation</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant cells</subject><subject>plant proteins</subject><subject>Plants</subject><subject>Plants and fungi</subject><subject>Pseudomonas syringae pv. syringae</subject><subject>RNA</subject><subject>substrates</subject><subject>tyrosine</subject><subject>Tyrosine - metabolism</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEFv1DAQRiMEokvhyBGIhMQtMI7tODlWFVCkShyg52hiTxaXrBM8TqUe-O94yaqC01j6nt9ovqJ4KeC9ADAfGEDVGkBDo9tHxU4oWVc1qPZxsQPIb-ikPiueMd8C5NCYp8WZAN3VIM2u-H2FcfGh9MGtlrg8-DTvKVRok7_DRK5c4pwoEz99QKbyb-DTfenW6MO-dDRSsFRG4mUOnBWZvYg4eDcv7LlMP3DyGLDklRcK7OdQ2nVKa_7xvHgy4sT04jTPi5tPH79fXlXXXz9_uby4rqxsZKo6JENkpLOjGaF1nRqccKRVowZjdK1RO0tyHLoBNDrRWCcQhSGhaquokefFu82bj_m1Eqf-4NnSNGGgeeW-6aTqlJQZrDbQxpk50tgv0R8w3vcC-mPf_X99Z_71SbwOB3L_0FvBGXh7ApAtTmPEYD0_cF3eKY6aVxt1y2mOD2ktZatbdbS82fIR5x73MRtuvtUgJNSdyi0Y-QcETZ4N</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>Desikan, R</creator><creator>Clarke, A</creator><creator>Atherfold, P</creator><creator>Hancock, J.T</creator><creator>Neill, S.J</creator><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991101</creationdate><title>Harpin induces mitogen-activated protein kinase activity during defence responses in Arabidopsis thaliana suspension cultures</title><author>Desikan, R ; Clarke, A ; Atherfold, P ; Hancock, J.T ; Neill, S.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-9ae7ee73dcf7f08d94bd1de5464b77525a5dce3fb9b05ad16cd1aa17e142c4e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Antibodies</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Arabidopsis - cytology</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis thaliana</topic><topic>Autoecology</topic><topic>Bacterial Outer Membrane Proteins - pharmacology</topic><topic>bacterial proteins</topic><topic>Biological and medical sciences</topic><topic>cell suspension culture</topic><topic>Cells, Cultured</topic><topic>defense mechanisms</topic><topic>Enzyme Activation</topic><topic>enzyme activity</topic><topic>Enzyme Induction</topic><topic>enzyme inhibitors</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Gene expression</topic><topic>Generalities. Disease free stocks</topic><topic>Hypersensitive response</topic><topic>immunochemistry</topic><topic>Mitogen-Activated Protein Kinases - antagonists & inhibitors</topic><topic>Mitogen-Activated Protein Kinases - biosynthesis</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>mitogens</topic><topic>molecular weight</topic><topic>Phosphatases</topic><topic>Phosphorylation</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant cells</topic><topic>plant proteins</topic><topic>Plants</topic><topic>Plants and fungi</topic><topic>Pseudomonas syringae pv. syringae</topic><topic>RNA</topic><topic>substrates</topic><topic>tyrosine</topic><topic>Tyrosine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Desikan, R</creatorcontrib><creatorcontrib>Clarke, A</creatorcontrib><creatorcontrib>Atherfold, P</creatorcontrib><creatorcontrib>Hancock, J.T</creatorcontrib><creatorcontrib>Neill, S.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Desikan, R</au><au>Clarke, A</au><au>Atherfold, P</au><au>Hancock, J.T</au><au>Neill, S.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Harpin induces mitogen-activated protein kinase activity during defence responses in Arabidopsis thaliana suspension cultures</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>210</volume><issue>1</issue><spage>97</spage><epage>103</epage><pages>97-103</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Elicitation of Arabidopsis thaliana (L.) Heynh. suspension cultures with the bacterial protein harpin (from Pseudomonas syringae pv. syringae) induced the activation of two kinases of 39 and 44 kDa, as demonstrated by in-gel kinase assays using myelin basic protein (MBP) as a substrate. Both these kinases appeared to be tyrosine-phosphorylated upon activation, as demonstrated by treatment with tyrosine phosphatase and immunoprecipitation using an anti-phosphotyrosine monoclonal antibody. An inhibitor of mammalian mitogen-activated protein kinase (MAPK) activation, PD98059, inhibited harpin-induced MBPK activation, but did not inhibit the activity of these kinases. PD98059 also inhibited harpin-induced programmed cell death and defence gene expression, suggesting the involvement of harpin-induced MAPKs in defence responses in Arabidopsis thaliana.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>10592037</pmid><doi>10.1007/s004250050658</doi><tpages>7</tpages></addata></record> |
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| subjects | Animal and plant ecology Animal, plant and microbial ecology Antibodies Apoptosis Apoptosis - drug effects Arabidopsis - cytology Arabidopsis - enzymology Arabidopsis thaliana Autoecology Bacterial Outer Membrane Proteins - pharmacology bacterial proteins Biological and medical sciences cell suspension culture Cells, Cultured defense mechanisms Enzyme Activation enzyme activity Enzyme Induction enzyme inhibitors Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Gels Gene expression Generalities. Disease free stocks Hypersensitive response immunochemistry Mitogen-Activated Protein Kinases - antagonists & inhibitors Mitogen-Activated Protein Kinases - biosynthesis Mitogen-Activated Protein Kinases - metabolism mitogens molecular weight Phosphatases Phosphorylation Phytopathology. Animal pests. Plant and forest protection Plant cells plant proteins Plants Plants and fungi Pseudomonas syringae pv. syringae RNA substrates tyrosine Tyrosine - metabolism |
| title | Harpin induces mitogen-activated protein kinase activity during defence responses in Arabidopsis thaliana suspension cultures |
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