Platelet Activating Factor Production and Proinflammatory Gene Expression in Endotoxin-Challenged Bovine Mammary Endothelial Cells
The bovine mammary gland responds to gram-negative pathogens by stimulating the production of cytokines and other proinflammatory mediators that orchestrate the migration of leukocytes into tissues. Platelet activating factor (PAF), interleukin 1β (IL-1β), IL-8, and intercellular adhesion molecule 1...
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| Veröffentlicht in: | Journal of dairy science 2008-08, Vol.91 (8), p.3067-3078 |
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| creator | Corl, C.M. Gandy, J.C. Sordillo, L.M. |
| description | The bovine mammary gland responds to gram-negative pathogens by stimulating the production of cytokines and other proinflammatory mediators that orchestrate the migration of leukocytes into tissues. Platelet activating factor (PAF), interleukin 1β (IL-1β), IL-8, and intercellular adhesion molecule 1 (ICAM1) are among the several inflammatory factors involved in the early activation and migration of leukocytes into the mammary gland during the initial stages of coliform mastitis. Several different cell types within the mammary gland are capable of expressing these potent pro-inflammatory mediators. The objective of this study was to characterize the expression profile of vascular-derived inflammatory molecules that may play a role in the pathogenesis of bovine coliform mastitis. Isolated bovine mammary gland endothelial cells were stimulated in culture for up to 12h with endotoxin obtained from Escherichia coli, and the temporal expression of proinflammatory cytokines and adhesion molecules relative to endogenous PAF biosynthesis was evaluated. Results from the in vitro time course experiment showed that vascular-derived PAF biosynthesis began as early as 30min and peaked at 1h following endotoxin challenge. The biosynthesis of PAF preceded the endotoxin-induced IL-1β, IL-8, and ICAM1 mRNA expression that increased after 1h and reached peak expression between 4 and 12h following stimulation. Inhibiting the effects of endogenous PAF with a receptor antagonist suggests that vascular-derived PAF is an early proinflammatory mediator that plays at least a partial role in the subsequent expression of IL-1β, IL-8, and ICAM1 during endotoxin challenge. Furthermore, endotoxin-induced PAF biosynthesis by bovine mammary gland endothelial cells is regulated to some extent by phospholipase D activity and phosphatidic acid production. The results from this study support the contention that mammary gland endothelial cells can contribute to the production of important proinflammatory mediators that are typically associated with coliform mastitis. |
| doi_str_mv | 10.3168/jds.2008-1066 |
| format | Article |
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Platelet activating factor (PAF), interleukin 1β (IL-1β), IL-8, and intercellular adhesion molecule 1 (ICAM1) are among the several inflammatory factors involved in the early activation and migration of leukocytes into the mammary gland during the initial stages of coliform mastitis. Several different cell types within the mammary gland are capable of expressing these potent pro-inflammatory mediators. The objective of this study was to characterize the expression profile of vascular-derived inflammatory molecules that may play a role in the pathogenesis of bovine coliform mastitis. Isolated bovine mammary gland endothelial cells were stimulated in culture for up to 12h with endotoxin obtained from Escherichia coli, and the temporal expression of proinflammatory cytokines and adhesion molecules relative to endogenous PAF biosynthesis was evaluated. Results from the in vitro time course experiment showed that vascular-derived PAF biosynthesis began as early as 30min and peaked at 1h following endotoxin challenge. The biosynthesis of PAF preceded the endotoxin-induced IL-1β, IL-8, and ICAM1 mRNA expression that increased after 1h and reached peak expression between 4 and 12h following stimulation. Inhibiting the effects of endogenous PAF with a receptor antagonist suggests that vascular-derived PAF is an early proinflammatory mediator that plays at least a partial role in the subsequent expression of IL-1β, IL-8, and ICAM1 during endotoxin challenge. Furthermore, endotoxin-induced PAF biosynthesis by bovine mammary gland endothelial cells is regulated to some extent by phospholipase D activity and phosphatidic acid production. The results from this study support the contention that mammary gland endothelial cells can contribute to the production of important proinflammatory mediators that are typically associated with coliform mastitis.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.2008-1066</identifier><identifier>PMID: 18650283</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Elsevier Inc</publisher><subject>1-Alkyl-2-acetylglycerophosphocholine Esterase - metabolism ; Animal productions ; Animals ; Biological and medical sciences ; bovine mastitis ; Cattle - metabolism ; Cattle - physiology ; Cells, Cultured ; coliform bacteria ; cows ; cytokine ; cytokines ; Cytokines - genetics ; endothelial cells ; Endothelial Cells - drug effects ; Endothelial Cells - enzymology ; Endothelial Cells - metabolism ; endothelium ; endotoxins ; Endotoxins - pharmacology ; Escherichia coli - chemistry ; Female ; Food industries ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Regulation - drug effects ; inflammation ; Intercellular Adhesion Molecule-1 - genetics ; Intercellular Adhesion Molecule-1 - metabolism ; intercellular adhesion molecules ; interleukin-1 ; interleukin-8 ; leukocytes ; mammary glands ; Mammary Glands, Animal - drug effects ; Mammary Glands, Animal - enzymology ; Mammary Glands, Animal - metabolism ; mastitis ; messenger RNA ; Milk and cheese industries. Ice creams ; pathogenesis ; Phosphatidic Acids - metabolism ; Phospholipase D - metabolism ; Phospholipid Ethers - pharmacology ; platelet activating factor ; Platelet Activating Factor - metabolism ; Platelet Aggregation Inhibitors - pharmacology ; Terrestrial animal productions ; Time Factors ; Vertebrates</subject><ispartof>Journal of dairy science, 2008-08, Vol.91 (8), p.3067-3078</ispartof><rights>2008 American Dairy Science Association</rights><rights>2008 INIST-CNRS</rights><rights>Copyright American Dairy Science Association Aug 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-10dc927818f2343902995240d045654cb5ecbde5750f3de580a93620f90256893</citedby><cites>FETCH-LOGICAL-c490t-10dc927818f2343902995240d045654cb5ecbde5750f3de580a93620f90256893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.2008-1066$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20540878$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18650283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Corl, C.M.</creatorcontrib><creatorcontrib>Gandy, J.C.</creatorcontrib><creatorcontrib>Sordillo, L.M.</creatorcontrib><title>Platelet Activating Factor Production and Proinflammatory Gene Expression in Endotoxin-Challenged Bovine Mammary Endothelial Cells</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>The bovine mammary gland responds to gram-negative pathogens by stimulating the production of cytokines and other proinflammatory mediators that orchestrate the migration of leukocytes into tissues. Platelet activating factor (PAF), interleukin 1β (IL-1β), IL-8, and intercellular adhesion molecule 1 (ICAM1) are among the several inflammatory factors involved in the early activation and migration of leukocytes into the mammary gland during the initial stages of coliform mastitis. Several different cell types within the mammary gland are capable of expressing these potent pro-inflammatory mediators. The objective of this study was to characterize the expression profile of vascular-derived inflammatory molecules that may play a role in the pathogenesis of bovine coliform mastitis. Isolated bovine mammary gland endothelial cells were stimulated in culture for up to 12h with endotoxin obtained from Escherichia coli, and the temporal expression of proinflammatory cytokines and adhesion molecules relative to endogenous PAF biosynthesis was evaluated. Results from the in vitro time course experiment showed that vascular-derived PAF biosynthesis began as early as 30min and peaked at 1h following endotoxin challenge. The biosynthesis of PAF preceded the endotoxin-induced IL-1β, IL-8, and ICAM1 mRNA expression that increased after 1h and reached peak expression between 4 and 12h following stimulation. Inhibiting the effects of endogenous PAF with a receptor antagonist suggests that vascular-derived PAF is an early proinflammatory mediator that plays at least a partial role in the subsequent expression of IL-1β, IL-8, and ICAM1 during endotoxin challenge. Furthermore, endotoxin-induced PAF biosynthesis by bovine mammary gland endothelial cells is regulated to some extent by phospholipase D activity and phosphatidic acid production. The results from this study support the contention that mammary gland endothelial cells can contribute to the production of important proinflammatory mediators that are typically associated with coliform mastitis.</description><subject>1-Alkyl-2-acetylglycerophosphocholine Esterase - metabolism</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>bovine mastitis</subject><subject>Cattle - metabolism</subject><subject>Cattle - physiology</subject><subject>Cells, Cultured</subject><subject>coliform bacteria</subject><subject>cows</subject><subject>cytokine</subject><subject>cytokines</subject><subject>Cytokines - genetics</subject><subject>endothelial cells</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - enzymology</subject><subject>Endothelial Cells - metabolism</subject><subject>endothelium</subject><subject>endotoxins</subject><subject>Endotoxins - pharmacology</subject><subject>Escherichia coli - chemistry</subject><subject>Female</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>inflammation</subject><subject>Intercellular Adhesion Molecule-1 - genetics</subject><subject>Intercellular Adhesion Molecule-1 - metabolism</subject><subject>intercellular adhesion molecules</subject><subject>interleukin-1</subject><subject>interleukin-8</subject><subject>leukocytes</subject><subject>mammary glands</subject><subject>Mammary Glands, Animal - drug effects</subject><subject>Mammary Glands, Animal - enzymology</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>mastitis</subject><subject>messenger RNA</subject><subject>Milk and cheese industries. Ice creams</subject><subject>pathogenesis</subject><subject>Phosphatidic Acids - metabolism</subject><subject>Phospholipase D - metabolism</subject><subject>Phospholipid Ethers - pharmacology</subject><subject>platelet activating factor</subject><subject>Platelet Activating Factor - metabolism</subject><subject>Platelet Aggregation Inhibitors - pharmacology</subject><subject>Terrestrial animal productions</subject><subject>Time Factors</subject><subject>Vertebrates</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUFvEzEQhVcIREPhyBVWSHDbMrbXu_axRGlBKqIS9Gw5tjdx5NjB3oRy5ZczSyIqIXGyxv7med68qnpJ4IKRTrzf2HJBAURDoOseVTPCKW8YkeJxNQOgtAEG9Kx6VsoGS0KBP63OiOg4UMFm1a_boEcX3FhfmtEf9Ojjqr7SZky5vs3J7vE2xVpHO5U-DkFvtxpff9bXLrp6cb_LrpSJ8bFeRJvGdO9jM1_rEFxcOVt_SAeP5OepEdv-MGsXvA713IVQnldPBh2Ke3E6z6u7q8W3-cfm5sv1p_nlTWNaCSP6s0bSXhAxUNYyCVRKTluw0PKOt2bJnVlax3sOA8NTgJasozAgyTsh2Xn17qi7y-n73pVRbX0xOIGOLu2L6iRre8JbBN_8A27SPkecTRHJBScSBELNETI5lZLdoHbZTwYVATUlozAZNSWjpmSQf3US3S-3zj7QpygQeHsCdDE6DFlH48tfDoNrQfTiwcbar9Y_fHaqbHHXKEumLyVRQjHoegRfH8FBJ6VXGcXuvlIgDEASwThHoj8SDtd-8C6rYryLxlmUNaOyyf_HzW8Keb9F</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Corl, C.M.</creator><creator>Gandy, J.C.</creator><creator>Sordillo, L.M.</creator><general>Elsevier Inc</general><general>American Dairy Science Association</general><general>Am Dairy Sci Assoc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20080801</creationdate><title>Platelet Activating Factor Production and Proinflammatory Gene Expression in Endotoxin-Challenged Bovine Mammary Endothelial Cells</title><author>Corl, C.M. ; Gandy, J.C. ; Sordillo, L.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-10dc927818f2343902995240d045654cb5ecbde5750f3de580a93620f90256893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>1-Alkyl-2-acetylglycerophosphocholine Esterase - metabolism</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>bovine mastitis</topic><topic>Cattle - metabolism</topic><topic>Cattle - physiology</topic><topic>Cells, Cultured</topic><topic>coliform bacteria</topic><topic>cows</topic><topic>cytokine</topic><topic>cytokines</topic><topic>Cytokines - genetics</topic><topic>endothelial cells</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - enzymology</topic><topic>Endothelial Cells - metabolism</topic><topic>endothelium</topic><topic>endotoxins</topic><topic>Endotoxins - pharmacology</topic><topic>Escherichia coli - chemistry</topic><topic>Female</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>inflammation</topic><topic>Intercellular Adhesion Molecule-1 - genetics</topic><topic>Intercellular Adhesion Molecule-1 - metabolism</topic><topic>intercellular adhesion molecules</topic><topic>interleukin-1</topic><topic>interleukin-8</topic><topic>leukocytes</topic><topic>mammary glands</topic><topic>Mammary Glands, Animal - drug effects</topic><topic>Mammary Glands, Animal - enzymology</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>mastitis</topic><topic>messenger RNA</topic><topic>Milk and cheese industries. Ice creams</topic><topic>pathogenesis</topic><topic>Phosphatidic Acids - metabolism</topic><topic>Phospholipase D - metabolism</topic><topic>Phospholipid Ethers - pharmacology</topic><topic>platelet activating factor</topic><topic>Platelet Activating Factor - metabolism</topic><topic>Platelet Aggregation Inhibitors - pharmacology</topic><topic>Terrestrial animal productions</topic><topic>Time Factors</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Corl, C.M.</creatorcontrib><creatorcontrib>Gandy, J.C.</creatorcontrib><creatorcontrib>Sordillo, L.M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Corl, C.M.</au><au>Gandy, J.C.</au><au>Sordillo, L.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet Activating Factor Production and Proinflammatory Gene Expression in Endotoxin-Challenged Bovine Mammary Endothelial Cells</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>91</volume><issue>8</issue><spage>3067</spage><epage>3078</epage><pages>3067-3078</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><coden>JDSCAE</coden><abstract>The bovine mammary gland responds to gram-negative pathogens by stimulating the production of cytokines and other proinflammatory mediators that orchestrate the migration of leukocytes into tissues. Platelet activating factor (PAF), interleukin 1β (IL-1β), IL-8, and intercellular adhesion molecule 1 (ICAM1) are among the several inflammatory factors involved in the early activation and migration of leukocytes into the mammary gland during the initial stages of coliform mastitis. Several different cell types within the mammary gland are capable of expressing these potent pro-inflammatory mediators. The objective of this study was to characterize the expression profile of vascular-derived inflammatory molecules that may play a role in the pathogenesis of bovine coliform mastitis. Isolated bovine mammary gland endothelial cells were stimulated in culture for up to 12h with endotoxin obtained from Escherichia coli, and the temporal expression of proinflammatory cytokines and adhesion molecules relative to endogenous PAF biosynthesis was evaluated. Results from the in vitro time course experiment showed that vascular-derived PAF biosynthesis began as early as 30min and peaked at 1h following endotoxin challenge. The biosynthesis of PAF preceded the endotoxin-induced IL-1β, IL-8, and ICAM1 mRNA expression that increased after 1h and reached peak expression between 4 and 12h following stimulation. Inhibiting the effects of endogenous PAF with a receptor antagonist suggests that vascular-derived PAF is an early proinflammatory mediator that plays at least a partial role in the subsequent expression of IL-1β, IL-8, and ICAM1 during endotoxin challenge. Furthermore, endotoxin-induced PAF biosynthesis by bovine mammary gland endothelial cells is regulated to some extent by phospholipase D activity and phosphatidic acid production. The results from this study support the contention that mammary gland endothelial cells can contribute to the production of important proinflammatory mediators that are typically associated with coliform mastitis.</abstract><cop>Savoy, IL</cop><pub>Elsevier Inc</pub><pmid>18650283</pmid><doi>10.3168/jds.2008-1066</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Alkyl-2-acetylglycerophosphocholine Esterase - metabolism Animal productions Animals Biological and medical sciences bovine mastitis Cattle - metabolism Cattle - physiology Cells, Cultured coliform bacteria cows cytokine cytokines Cytokines - genetics endothelial cells Endothelial Cells - drug effects Endothelial Cells - enzymology Endothelial Cells - metabolism endothelium endotoxins Endotoxins - pharmacology Escherichia coli - chemistry Female Food industries Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation - drug effects inflammation Intercellular Adhesion Molecule-1 - genetics Intercellular Adhesion Molecule-1 - metabolism intercellular adhesion molecules interleukin-1 interleukin-8 leukocytes mammary glands Mammary Glands, Animal - drug effects Mammary Glands, Animal - enzymology Mammary Glands, Animal - metabolism mastitis messenger RNA Milk and cheese industries. Ice creams pathogenesis Phosphatidic Acids - metabolism Phospholipase D - metabolism Phospholipid Ethers - pharmacology platelet activating factor Platelet Activating Factor - metabolism Platelet Aggregation Inhibitors - pharmacology Terrestrial animal productions Time Factors Vertebrates |
| title | Platelet Activating Factor Production and Proinflammatory Gene Expression in Endotoxin-Challenged Bovine Mammary Endothelial Cells |
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