Impact of stromal cell composition on BMP-induced chondrogenic differentiation of mouse bone marrow derived mesenchymal cells

Chondrogenic differentiation in mesenchymal stromal cells (MSCs) has been actively studied due to their potential use in mesenchymal tissue repair. Our goal was to develop a simple isolation protocol for adherent mouse MSCs to simultaneously clear off hematopoietic cells and expand to obtain enough...

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Veröffentlicht in:	Experimental cell research 2008-08, Vol.314 (13), p.2400-2410
Hauptverfasser:	Taipaleenmäki, Hanna, Suomi, Salla, Hentunen, Teuvo, Laitala-Leinonen, Tiina, Säämänen, Anna-Marja
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipogenesis - drug effects Adipogenesis - genetics Animals Biotechnology Bone marrow Bone Marrow Cells - drug effects Bone Marrow Cells - physiology Bone marrow stroma Bone morphogenetic protein Bone Morphogenetic Proteins - genetics Bone Morphogenetic Proteins - metabolism Bone Morphogenetic Proteins - pharmacology Cell Count Cell culture Cell Culture Techniques Cell Differentiation - drug effects Cell Differentiation - genetics Cells, Cultured Cellular biology Chondrogenesis Chondrogenesis - drug effects Chondrogenesis - genetics Embryo, Mammalian Gene Expression Profiling Gene Expression Regulation, Developmental Growth Plate - embryology Growth Plate - metabolism Male Mesenchymal stem cell Mesenchymal stromal cell Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - physiology Mice Mice, Inbred C57BL Mice, Inbred DBA Mouse Pellet culture Rodents Sca-1 Stem cell antigen-1 Stromal Cells - cytology Stromal Cells - physiology Terminal differentiation Time Factors Tissue Distribution
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