Impact of stromal cell composition on BMP-induced chondrogenic differentiation of mouse bone marrow derived mesenchymal cells
Chondrogenic differentiation in mesenchymal stromal cells (MSCs) has been actively studied due to their potential use in mesenchymal tissue repair. Our goal was to develop a simple isolation protocol for adherent mouse MSCs to simultaneously clear off hematopoietic cells and expand to obtain enough...
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| Veröffentlicht in: | Experimental cell research 2008-08, Vol.314 (13), p.2400-2410 |
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| creator | Taipaleenmäki, Hanna Suomi, Salla Hentunen, Teuvo Laitala-Leinonen, Tiina Säämänen, Anna-Marja |
| description | Chondrogenic differentiation in mesenchymal stromal cells (MSCs) has been actively studied due to their potential use in mesenchymal tissue repair. Our goal was to develop a simple isolation protocol for adherent mouse MSCs to simultaneously clear off hematopoietic cells and expand to obtain enough starting material for differentiation studies. CD34 and CD45 expressing cells were rapidly removed by inhibiting growth of hematopoietic cells to yield short-term selected (STS) cells. Further passaging enriched more primitive, uniformly Sca-1 expressing, long-term selected (LTS) cells. The efficacy of several BMPs to induce chondrogenesis in pellet culture was compared in STS and LTS cells. In STS cells, chondrogenesis progressed rapidly to terminal differentiation while LTS cells differentiated at a slower rate with no hypertrophy. In LTS cells, rhBMP homodimers -2, -4, -6 and rhBMP2/7 heterodimer were effective enhancers of chondrogenesis over that of rhBMP-5 and -7. In STS cells, rhBMP-2 and rhBMP-7 supported rapid chondrogenesis and terminal differentiation over that of rhBMP-6. These data indicate the impact of stromal cell composition on the chondrogenic differentiation profile, which is an important aspect to be considered when standardizing differentiation assay conditions as well as developing MSC based cartilage repair technologies. |
| doi_str_mv | 10.1016/j.yexcr.2008.04.019 |
| format | Article |
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Our goal was to develop a simple isolation protocol for adherent mouse MSCs to simultaneously clear off hematopoietic cells and expand to obtain enough starting material for differentiation studies. CD34 and CD45 expressing cells were rapidly removed by inhibiting growth of hematopoietic cells to yield short-term selected (STS) cells. Further passaging enriched more primitive, uniformly Sca-1 expressing, long-term selected (LTS) cells. The efficacy of several BMPs to induce chondrogenesis in pellet culture was compared in STS and LTS cells. In STS cells, chondrogenesis progressed rapidly to terminal differentiation while LTS cells differentiated at a slower rate with no hypertrophy. In LTS cells, rhBMP homodimers -2, -4, -6 and rhBMP2/7 heterodimer were effective enhancers of chondrogenesis over that of rhBMP-5 and -7. In STS cells, rhBMP-2 and rhBMP-7 supported rapid chondrogenesis and terminal differentiation over that of rhBMP-6. These data indicate the impact of stromal cell composition on the chondrogenic differentiation profile, which is an important aspect to be considered when standardizing differentiation assay conditions as well as developing MSC based cartilage repair technologies.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2008.04.019</identifier><identifier>PMID: 18565511</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipogenesis - drug effects ; Adipogenesis - genetics ; Animals ; Biotechnology ; Bone marrow ; Bone Marrow Cells - drug effects ; Bone Marrow Cells - physiology ; Bone marrow stroma ; Bone morphogenetic protein ; Bone Morphogenetic Proteins - genetics ; Bone Morphogenetic Proteins - metabolism ; Bone Morphogenetic Proteins - pharmacology ; Cell Count ; Cell culture ; Cell Culture Techniques ; Cell Differentiation - drug effects ; Cell Differentiation - genetics ; Cells, Cultured ; Cellular biology ; Chondrogenesis ; Chondrogenesis - drug effects ; Chondrogenesis - genetics ; Embryo, Mammalian ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Growth Plate - embryology ; Growth Plate - metabolism ; Male ; Mesenchymal stem cell ; Mesenchymal stromal cell ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - physiology ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Mouse ; Pellet culture ; Rodents ; Sca-1 ; Stem cell antigen-1 ; Stromal Cells - cytology ; Stromal Cells - physiology ; Terminal differentiation ; Time Factors ; Tissue Distribution</subject><ispartof>Experimental cell research, 2008-08, Vol.314 (13), p.2400-2410</ispartof><rights>2008 Elsevier Inc.</rights><rights>Copyright © 2008 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-8e4af647e50399ae922740bba4c2c678c3bf68fa45ee77b3b33c9b3b3c43af8e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yexcr.2008.04.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18565511$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taipaleenmäki, Hanna</creatorcontrib><creatorcontrib>Suomi, Salla</creatorcontrib><creatorcontrib>Hentunen, Teuvo</creatorcontrib><creatorcontrib>Laitala-Leinonen, Tiina</creatorcontrib><creatorcontrib>Säämänen, Anna-Marja</creatorcontrib><title>Impact of stromal cell composition on BMP-induced chondrogenic differentiation of mouse bone marrow derived mesenchymal cells</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Chondrogenic differentiation in mesenchymal stromal cells (MSCs) has been actively studied due to their potential use in mesenchymal tissue repair. Our goal was to develop a simple isolation protocol for adherent mouse MSCs to simultaneously clear off hematopoietic cells and expand to obtain enough starting material for differentiation studies. CD34 and CD45 expressing cells were rapidly removed by inhibiting growth of hematopoietic cells to yield short-term selected (STS) cells. Further passaging enriched more primitive, uniformly Sca-1 expressing, long-term selected (LTS) cells. The efficacy of several BMPs to induce chondrogenesis in pellet culture was compared in STS and LTS cells. In STS cells, chondrogenesis progressed rapidly to terminal differentiation while LTS cells differentiated at a slower rate with no hypertrophy. In LTS cells, rhBMP homodimers -2, -4, -6 and rhBMP2/7 heterodimer were effective enhancers of chondrogenesis over that of rhBMP-5 and -7. In STS cells, rhBMP-2 and rhBMP-7 supported rapid chondrogenesis and terminal differentiation over that of rhBMP-6. These data indicate the impact of stromal cell composition on the chondrogenic differentiation profile, which is an important aspect to be considered when standardizing differentiation assay conditions as well as developing MSC based cartilage repair technologies.</description><subject>Adipogenesis - drug effects</subject><subject>Adipogenesis - genetics</subject><subject>Animals</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bone Marrow Cells - physiology</subject><subject>Bone marrow stroma</subject><subject>Bone morphogenetic protein</subject><subject>Bone Morphogenetic Proteins - genetics</subject><subject>Bone Morphogenetic Proteins - metabolism</subject><subject>Bone Morphogenetic Proteins - pharmacology</subject><subject>Cell Count</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - genetics</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Chondrogenesis</subject><subject>Chondrogenesis - drug effects</subject><subject>Chondrogenesis - genetics</subject><subject>Embryo, Mammalian</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Growth Plate - embryology</subject><subject>Growth Plate - metabolism</subject><subject>Male</subject><subject>Mesenchymal stem cell</subject><subject>Mesenchymal stromal cell</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred DBA</subject><subject>Mouse</subject><subject>Pellet culture</subject><subject>Rodents</subject><subject>Sca-1</subject><subject>Stem cell antigen-1</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - physiology</subject><subject>Terminal differentiation</subject><subject>Time Factors</subject><subject>Tissue Distribution</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EotPCL0BCFgt2CX4m9oIFVFAqFcEC1pbjXFOPJvZgJ4VZ8N9xmEFILECyfDffuY9zEHpCSUsJ7V5s2wN8d7llhKiWiJZQfQ9tKNGkYYKx-2hDCBWNUKw_Q-elbEkFFe0eojOqZCclpRv043raWzfj5HGZc5rsDjvY1S9N-1TCHFLE9b1-_7EJcVwcjNjdpjjm9AVicHgM3kOGOAd7ZD2e0lIADykCnmzO6RseIYe7qpygQHS3h99TyiP0wNtdgceneoE-v33z6fJdc_Ph6vry1U3jBJVzo0BY34keJOFaW9CM9YIMgxWOua5Xjg--U94KCdD3Ax84d3otTnDrFfAL9PzYd5_T1wXKbKZQ1g1shLqt6TQXUnH9X5CRam01t4LP_gK3acmxHmGoFp2SRK7d-BFyOZWSwZt9DtWTg6HErBmarfmVoVkzNESYmmFVPT21XoYJxj-aU2gVeHkEoFp2FyCb4kI1FsaQwc1mTOGfA34C2lqwiQ</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Taipaleenmäki, Hanna</creator><creator>Suomi, Salla</creator><creator>Hentunen, Teuvo</creator><creator>Laitala-Leinonen, Tiina</creator><creator>Säämänen, Anna-Marja</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>20080801</creationdate><title>Impact of stromal cell composition on BMP-induced chondrogenic differentiation of mouse bone marrow derived mesenchymal cells</title><author>Taipaleenmäki, Hanna ; Suomi, Salla ; Hentunen, Teuvo ; Laitala-Leinonen, Tiina ; Säämänen, Anna-Marja</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-8e4af647e50399ae922740bba4c2c678c3bf68fa45ee77b3b33c9b3b3c43af8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adipogenesis - drug effects</topic><topic>Adipogenesis - genetics</topic><topic>Animals</topic><topic>Biotechnology</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bone Marrow Cells - physiology</topic><topic>Bone marrow stroma</topic><topic>Bone morphogenetic protein</topic><topic>Bone Morphogenetic Proteins - genetics</topic><topic>Bone Morphogenetic Proteins - metabolism</topic><topic>Bone Morphogenetic Proteins - pharmacology</topic><topic>Cell Count</topic><topic>Cell culture</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - genetics</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>Chondrogenesis</topic><topic>Chondrogenesis - drug effects</topic><topic>Chondrogenesis - genetics</topic><topic>Embryo, Mammalian</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Growth Plate - embryology</topic><topic>Growth Plate - metabolism</topic><topic>Male</topic><topic>Mesenchymal stem cell</topic><topic>Mesenchymal stromal cell</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred DBA</topic><topic>Mouse</topic><topic>Pellet culture</topic><topic>Rodents</topic><topic>Sca-1</topic><topic>Stem cell antigen-1</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - physiology</topic><topic>Terminal differentiation</topic><topic>Time Factors</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taipaleenmäki, Hanna</creatorcontrib><creatorcontrib>Suomi, Salla</creatorcontrib><creatorcontrib>Hentunen, Teuvo</creatorcontrib><creatorcontrib>Laitala-Leinonen, Tiina</creatorcontrib><creatorcontrib>Säämänen, Anna-Marja</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taipaleenmäki, Hanna</au><au>Suomi, Salla</au><au>Hentunen, Teuvo</au><au>Laitala-Leinonen, Tiina</au><au>Säämänen, Anna-Marja</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of stromal cell composition on BMP-induced chondrogenic differentiation of mouse bone marrow derived mesenchymal cells</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>314</volume><issue>13</issue><spage>2400</spage><epage>2410</epage><pages>2400-2410</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Chondrogenic differentiation in mesenchymal stromal cells (MSCs) has been actively studied due to their potential use in mesenchymal tissue repair. Our goal was to develop a simple isolation protocol for adherent mouse MSCs to simultaneously clear off hematopoietic cells and expand to obtain enough starting material for differentiation studies. CD34 and CD45 expressing cells were rapidly removed by inhibiting growth of hematopoietic cells to yield short-term selected (STS) cells. Further passaging enriched more primitive, uniformly Sca-1 expressing, long-term selected (LTS) cells. The efficacy of several BMPs to induce chondrogenesis in pellet culture was compared in STS and LTS cells. In STS cells, chondrogenesis progressed rapidly to terminal differentiation while LTS cells differentiated at a slower rate with no hypertrophy. In LTS cells, rhBMP homodimers -2, -4, -6 and rhBMP2/7 heterodimer were effective enhancers of chondrogenesis over that of rhBMP-5 and -7. In STS cells, rhBMP-2 and rhBMP-7 supported rapid chondrogenesis and terminal differentiation over that of rhBMP-6. These data indicate the impact of stromal cell composition on the chondrogenic differentiation profile, which is an important aspect to be considered when standardizing differentiation assay conditions as well as developing MSC based cartilage repair technologies.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18565511</pmid><doi>10.1016/j.yexcr.2008.04.019</doi><tpages>11</tpages></addata></record> |
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| ispartof | Experimental cell research, 2008-08, Vol.314 (13), p.2400-2410 |
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| subjects | Adipogenesis - drug effects Adipogenesis - genetics Animals Biotechnology Bone marrow Bone Marrow Cells - drug effects Bone Marrow Cells - physiology Bone marrow stroma Bone morphogenetic protein Bone Morphogenetic Proteins - genetics Bone Morphogenetic Proteins - metabolism Bone Morphogenetic Proteins - pharmacology Cell Count Cell culture Cell Culture Techniques Cell Differentiation - drug effects Cell Differentiation - genetics Cells, Cultured Cellular biology Chondrogenesis Chondrogenesis - drug effects Chondrogenesis - genetics Embryo, Mammalian Gene Expression Profiling Gene Expression Regulation, Developmental Growth Plate - embryology Growth Plate - metabolism Male Mesenchymal stem cell Mesenchymal stromal cell Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - physiology Mice Mice, Inbred C57BL Mice, Inbred DBA Mouse Pellet culture Rodents Sca-1 Stem cell antigen-1 Stromal Cells - cytology Stromal Cells - physiology Terminal differentiation Time Factors Tissue Distribution |
| title | Impact of stromal cell composition on BMP-induced chondrogenic differentiation of mouse bone marrow derived mesenchymal cells |
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