In Vitro Hepatic Differentiation of Mesenchymal Stem Cells from Human Fetal Bone Marrow
We examined whether human fetal mesenchymal stem cells (FMSCs) derived from fetal bone marrow were able to differentiate into functional hepatocyte-like cells in vitro The surface phenotype of FMSCs was characterized by flow cytometry. To induce hepatic differentiation of FMSCs, we added hepatocyte...
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| Veröffentlicht in: | Journal of international medical research 2008-07, Vol.36 (4), p.721-727 |
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| creator | Wei, X Wang, CY Liu, QP Li, J Li, D Zhao, FT Lian, JQ Xie, YM Wang, PZ Bai, XF Jia, ZS |
| description | We examined whether human fetal mesenchymal stem cells (FMSCs) derived from fetal bone marrow were able to differentiate into functional hepatocyte-like cells in vitro The surface phenotype of FMSCs was characterized by flow cytometry. To induce hepatic differentiation of FMSCs, we added hepatocyte growth factor, basic fibroblast growth factor and oncostatin M into the cell culture medium. After 21 days of hepatocyte induction, FMSCs expressed the hepatocyte-specific markers, α-fetoprotein and cytokeratin 18, as demonstrated by immunofluorescence staining. Differentiated FMSCs also demonstrated in vitro functions characteristic of liver cells, including albumin production, urea secretion and glycogen storage. In conclusion, fetal bone marrow-derived FMSCs are able to differentiate into functional hepatocyte-like cells and may serve as a source of cells for liver disease therapy. |
| doi_str_mv | 10.1177/147323000803600414 |
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To induce hepatic differentiation of FMSCs, we added hepatocyte growth factor, basic fibroblast growth factor and oncostatin M into the cell culture medium. After 21 days of hepatocyte induction, FMSCs expressed the hepatocyte-specific markers, α-fetoprotein and cytokeratin 18, as demonstrated by immunofluorescence staining. Differentiated FMSCs also demonstrated in vitro functions characteristic of liver cells, including albumin production, urea secretion and glycogen storage. In conclusion, fetal bone marrow-derived FMSCs are able to differentiate into functional hepatocyte-like cells and may serve as a source of cells for liver disease therapy.</description><identifier>ISSN: 0300-0605</identifier><identifier>EISSN: 1473-2300</identifier><identifier>DOI: 10.1177/147323000803600414</identifier><identifier>PMID: 18652768</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Albumins - metabolism ; Bone Marrow Cells - cytology ; Bone Marrow Cells - physiology ; Cell Differentiation - physiology ; Cell Lineage ; Cells, Cultured ; Female ; Fetal Stem Cells - cytology ; Fetal Stem Cells - physiology ; Flow Cytometry ; Hepatocytes - cytology ; Hepatocytes - physiology ; Humans ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Pregnancy ; Urea - metabolism</subject><ispartof>Journal of international medical research, 2008-07, Vol.36 (4), p.721-727</ispartof><rights>2008 SAGE Publications</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-f5f7e22ba082743e256fe589a74f4a0aa7eacbc7d3e600d760d99006aa1b9813</citedby><cites>FETCH-LOGICAL-c407t-f5f7e22ba082743e256fe589a74f4a0aa7eacbc7d3e600d760d99006aa1b9813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/147323000803600414$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/147323000803600414$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21964,27851,27922,27923,44943,45331</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/147323000803600414?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18652768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, X</creatorcontrib><creatorcontrib>Wang, CY</creatorcontrib><creatorcontrib>Liu, QP</creatorcontrib><creatorcontrib>Li, J</creatorcontrib><creatorcontrib>Li, D</creatorcontrib><creatorcontrib>Zhao, FT</creatorcontrib><creatorcontrib>Lian, JQ</creatorcontrib><creatorcontrib>Xie, YM</creatorcontrib><creatorcontrib>Wang, PZ</creatorcontrib><creatorcontrib>Bai, XF</creatorcontrib><creatorcontrib>Jia, ZS</creatorcontrib><title>In Vitro Hepatic Differentiation of Mesenchymal Stem Cells from Human Fetal Bone Marrow</title><title>Journal of international medical research</title><addtitle>J Int Med Res</addtitle><description>We examined whether human fetal mesenchymal stem cells (FMSCs) derived from fetal bone marrow were able to differentiate into functional hepatocyte-like cells in vitro The surface phenotype of FMSCs was characterized by flow cytometry. To induce hepatic differentiation of FMSCs, we added hepatocyte growth factor, basic fibroblast growth factor and oncostatin M into the cell culture medium. After 21 days of hepatocyte induction, FMSCs expressed the hepatocyte-specific markers, α-fetoprotein and cytokeratin 18, as demonstrated by immunofluorescence staining. Differentiated FMSCs also demonstrated in vitro functions characteristic of liver cells, including albumin production, urea secretion and glycogen storage. In conclusion, fetal bone marrow-derived FMSCs are able to differentiate into functional hepatocyte-like cells and may serve as a source of cells for liver disease therapy.</description><subject>Albumins - metabolism</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - physiology</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Lineage</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fetal Stem Cells - cytology</subject><subject>Fetal Stem Cells - physiology</subject><subject>Flow Cytometry</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - physiology</subject><subject>Humans</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Pregnancy</subject><subject>Urea - metabolism</subject><issn>0300-0605</issn><issn>1473-2300</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDFPwzAQhS0EoqXwBxiQJ7bQs-PYyQiF0kqtGKhgjJzkDKmauNiJUP89rlqJAYnp6e6-e7p7hFwzuGNMqTETKuYxAKQQSwDBxAkZ7pvRvntKhhAkAgnJgFx4vw4Ilwk_JwOWBlUyHZL3eUvf6s5ZOsOt7uqSPtbGoMO2q0NpW2oNXaLHtvzcNXpDXzts6AQ3G0-Nsw2d9Y1u6RS7MHuwLdKlds5-X5Izozcer446Iqvp02oyixYvz_PJ_SIqBaguMolRyHmhIeVKxMgTaTBJM62EERq0VqjLolRVjOHDSkmosgxAas2KLGXxiNwebLfOfvXou7ypfRmu0y3a3ucyi4VQWRxAfgBLZ713aPKtqxvtdjmDfJ9m_jfNsHRzdO-LBqvflWN8ARgfAK8_MF_b3rXh2f8sfwDwQXwR</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Wei, X</creator><creator>Wang, CY</creator><creator>Liu, QP</creator><creator>Li, J</creator><creator>Li, D</creator><creator>Zhao, FT</creator><creator>Lian, JQ</creator><creator>Xie, YM</creator><creator>Wang, PZ</creator><creator>Bai, XF</creator><creator>Jia, ZS</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080701</creationdate><title>In Vitro Hepatic Differentiation of Mesenchymal Stem Cells from Human Fetal Bone Marrow</title><author>Wei, X ; 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| subjects | Albumins - metabolism Bone Marrow Cells - cytology Bone Marrow Cells - physiology Cell Differentiation - physiology Cell Lineage Cells, Cultured Female Fetal Stem Cells - cytology Fetal Stem Cells - physiology Flow Cytometry Hepatocytes - cytology Hepatocytes - physiology Humans Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Pregnancy Urea - metabolism |
| title | In Vitro Hepatic Differentiation of Mesenchymal Stem Cells from Human Fetal Bone Marrow |
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