Antigen-specific cytokine responses in vaccinated Macaca nemestrina
: We describe a new surrogate assay for CD8 + T lymphocyte activity that has the capability of discriminating between cytotoxic T lymphocyte (CTL) activity and cytokine‐mediated suppressive activity. We applied this approach to two groups of Macaca nemestrina vaccinated with a minimally pathogenic s...
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| Veröffentlicht in: | Journal of medical primatology 1999-08, Vol.28 (4-5), p.181-189 |
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| container_end_page | 189 |
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| container_issue | 4-5 |
| container_start_page | 181 |
| container_title | Journal of medical primatology |
| container_volume | 28 |
| creator | Mulvania, Thera Lynch, John B. Robertson, Michael N. Greenberg, Philip D. Morton, William R. Mullins, James I. |
| description | : We describe a new surrogate assay for CD8 + T lymphocyte activity that has the capability of discriminating between cytotoxic T lymphocyte (CTL) activity and cytokine‐mediated suppressive activity. We applied this approach to two groups of Macaca nemestrina vaccinated with a minimally pathogenic strain of human immunodeficiency virus type 2 [HIV‐2 (HIV‐2KR)] as a model of an attenuated virus vaccine. Group 1 was then inoculated with a non‐infectious stock of a pathogenic strain, HIV‐2287. Both groups 1 and 2 were subsequently challenged with an infectious stock of HIV‐2287. Five out of six group 1 animals were protected against CD4 decline, whereas three out of six animals in group 2 were protected. Analysis of CTL responses demonstrated strong activity against HIV‐2KR‐Gag in group 1. It was determined that strong CTL responses correlate with antigen‐specific T‐helper (Th) type 1 responses. This antigen‐specific cytokine assay has the potential to better elucidate the functional mechanisms of CD8 + T‐cell‐mediated protection than traditional methods to date. |
| doi_str_mv | 10.1111/j.1600-0684.1999.tb00268.x |
| format | Article |
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We applied this approach to two groups of Macaca nemestrina vaccinated with a minimally pathogenic strain of human immunodeficiency virus type 2 [HIV‐2 (HIV‐2KR)] as a model of an attenuated virus vaccine. Group 1 was then inoculated with a non‐infectious stock of a pathogenic strain, HIV‐2287. Both groups 1 and 2 were subsequently challenged with an infectious stock of HIV‐2287. Five out of six group 1 animals were protected against CD4 decline, whereas three out of six animals in group 2 were protected. Analysis of CTL responses demonstrated strong activity against HIV‐2KR‐Gag in group 1. It was determined that strong CTL responses correlate with antigen‐specific T‐helper (Th) type 1 responses. 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We applied this approach to two groups of Macaca nemestrina vaccinated with a minimally pathogenic strain of human immunodeficiency virus type 2 [HIV‐2 (HIV‐2KR)] as a model of an attenuated virus vaccine. Group 1 was then inoculated with a non‐infectious stock of a pathogenic strain, HIV‐2287. Both groups 1 and 2 were subsequently challenged with an infectious stock of HIV‐2287. Five out of six group 1 animals were protected against CD4 decline, whereas three out of six animals in group 2 were protected. Analysis of CTL responses demonstrated strong activity against HIV‐2KR‐Gag in group 1. It was determined that strong CTL responses correlate with antigen‐specific T‐helper (Th) type 1 responses. This antigen‐specific cytokine assay has the potential to better elucidate the functional mechanisms of CD8 + T‐cell‐mediated protection than traditional methods to date.</description><subject>AIDS Vaccines</subject><subject>AIDS/HIV</subject><subject>Animals</subject><subject>CD4 antigen</subject><subject>CD4 Lymphocyte Count</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cytokines - immunology</subject><subject>cytotoxic T lymphocytes</subject><subject>Disease Models, Animal</subject><subject>HIV-2 - immunology</subject><subject>Human immunodeficiency virus 2</subject><subject>Humans</subject><subject>interferon-γ</subject><subject>interleukin-4</subject><subject>intracellular</subject><subject>Macaca nemestrina - immunology</subject><subject>RANTES</subject><subject>Simian Immunodeficiency Virus - immunology</subject><subject>Vaccination - veterinary</subject><issn>0047-2565</issn><issn>1600-0684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkFtLwzAUx4MoOi9fQYoP4kvryXJrfRDG8DbmBVH0LaTZqWRu7Ww63b69GR3ik2DykJD8zv8kP0KOKCQ0jNNxQiVADDLlCc2yLGlygK5Mk8UG6fxcbZIOAFdxV0ixQ3a9HwMA4xnfJjsURMZ4yjuk3ysb94Zl7GdoXeFsZJdN9e5KjGr0s6r06CNXRp_GWleaBkfRrbFhRiVO0Td1ONwnW4WZeDxYr3vk-fLiqX8dD--vbvq9YWw5p2ksipQpqhQwCUWmwlMAcjTpSBpuFaVWKCqpYChyK5XKVdcwTqUQiGneNQXbI8dt7qyuPuahuZ46b3EyMSVWc69l-BMP3w3gyZ8g5ZILkFkGAT1rUVtX3tdY6FntpqZeagp6ZVuP9UqpXinVK9t6bVsvQvHhus88n-LoV2mrNwDnLfDlJrj8R7Qe3D6ETQiI2wDnG1z8BJj6XUvFlNAvd1daDqR4fB0O9CX7BtIInS0</recordid><startdate>199908</startdate><enddate>199908</enddate><creator>Mulvania, Thera</creator><creator>Lynch, John B.</creator><creator>Robertson, Michael N.</creator><creator>Greenberg, Philip D.</creator><creator>Morton, William R.</creator><creator>Mullins, James I.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199908</creationdate><title>Antigen-specific cytokine responses in vaccinated Macaca nemestrina</title><author>Mulvania, Thera ; Lynch, John B. ; Robertson, Michael N. ; Greenberg, Philip D. ; Morton, William R. ; Mullins, James I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4418-5f8371770360f9734900bea8d6a4c711c5716153e5bc677b72a341655ee8b2af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>AIDS Vaccines</topic><topic>AIDS/HIV</topic><topic>Animals</topic><topic>CD4 antigen</topic><topic>CD4 Lymphocyte Count</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cytokines - immunology</topic><topic>cytotoxic T lymphocytes</topic><topic>Disease Models, Animal</topic><topic>HIV-2 - immunology</topic><topic>Human immunodeficiency virus 2</topic><topic>Humans</topic><topic>interferon-γ</topic><topic>interleukin-4</topic><topic>intracellular</topic><topic>Macaca nemestrina - immunology</topic><topic>RANTES</topic><topic>Simian Immunodeficiency Virus - immunology</topic><topic>Vaccination - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mulvania, Thera</creatorcontrib><creatorcontrib>Lynch, John B.</creatorcontrib><creatorcontrib>Robertson, Michael N.</creatorcontrib><creatorcontrib>Greenberg, Philip D.</creatorcontrib><creatorcontrib>Morton, William R.</creatorcontrib><creatorcontrib>Mullins, James I.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical primatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mulvania, Thera</au><au>Lynch, John B.</au><au>Robertson, Michael N.</au><au>Greenberg, Philip D.</au><au>Morton, William R.</au><au>Mullins, James I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigen-specific cytokine responses in vaccinated Macaca nemestrina</atitle><jtitle>Journal of medical primatology</jtitle><addtitle>J Med Primatol</addtitle><date>1999-08</date><risdate>1999</risdate><volume>28</volume><issue>4-5</issue><spage>181</spage><epage>189</epage><pages>181-189</pages><issn>0047-2565</issn><eissn>1600-0684</eissn><abstract>: We describe a new surrogate assay for CD8 + T lymphocyte activity that has the capability of discriminating between cytotoxic T lymphocyte (CTL) activity and cytokine‐mediated suppressive activity. 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This antigen‐specific cytokine assay has the potential to better elucidate the functional mechanisms of CD8 + T‐cell‐mediated protection than traditional methods to date.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10593484</pmid><doi>10.1111/j.1600-0684.1999.tb00268.x</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0047-2565 |
| ispartof | Journal of medical primatology, 1999-08, Vol.28 (4-5), p.181-189 |
| issn | 0047-2565 1600-0684 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | AIDS Vaccines AIDS/HIV Animals CD4 antigen CD4 Lymphocyte Count CD8-Positive T-Lymphocytes - immunology Cytokines - immunology cytotoxic T lymphocytes Disease Models, Animal HIV-2 - immunology Human immunodeficiency virus 2 Humans interferon-γ interleukin-4 intracellular Macaca nemestrina - immunology RANTES Simian Immunodeficiency Virus - immunology Vaccination - veterinary |
| title | Antigen-specific cytokine responses in vaccinated Macaca nemestrina |
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