Molecular Characterization of Extended-Spectrum β-Lactamases Produced by Clinical Isolates of Enterobacter cloacae from a Teaching Hospital in China
Of 59 clinical isolates of Enterobacter cloacae from a teaching hospital in Sichuan, China, 18 isolates were shown to be resistant to oxyimino cephalosporins and aztreonam. Enterobacterial repetitive consensus PCR revealed that these isolates comprised 7 distinct genotypes. The presence of plasmids...
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| Veröffentlicht in: | Japanese Journal of Infectious Diseases 2008/07/28, Vol.61(4), pp.286-289 |
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| creator | Liu, Gang Ling, Bao-Dong Zeng, Yun Lin, Li Xie, Yong-En Lei, Jun |
| description | Of 59 clinical isolates of Enterobacter cloacae from a teaching hospital in Sichuan, China, 18 isolates were shown to be resistant to oxyimino cephalosporins and aztreonam. Enterobacterial repetitive consensus PCR revealed that these isolates comprised 7 distinct genotypes. The presence of plasmids in the 18 clinical isolates was revealed by conjugational transfer of plasmids from E. cloacae to Escherichia coli with the further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing and β-lactamase isoelectric focusing indicated that the plasmids encoded blaSHV, blaCTX-M and/or blaTEM genes, including genes for CTX-M-22 (13 strains), TEM-1 (12 strains), TEM-29 (1 strain), TEM-141 (1 strain), TEM-157 (1 strain), SHV-5 (1 strain), SHV-12 (1 strain), and SHV-70 (1 strain). The widespread presence of extended-spectrum β-lactamases in E. cloacae isolated from the southwest of China was likely due to the dissemination of resistance plasmids with the predominant genotype of blaCTX-M-22. |
| doi_str_mv | 10.7883/yoken.JJID.2008.286 |
| format | Article |
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Enterobacterial repetitive consensus PCR revealed that these isolates comprised 7 distinct genotypes. The presence of plasmids in the 18 clinical isolates was revealed by conjugational transfer of plasmids from E. cloacae to Escherichia coli with the further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing and β-lactamase isoelectric focusing indicated that the plasmids encoded blaSHV, blaCTX-M and/or blaTEM genes, including genes for CTX-M-22 (13 strains), TEM-1 (12 strains), TEM-29 (1 strain), TEM-141 (1 strain), TEM-157 (1 strain), SHV-5 (1 strain), SHV-12 (1 strain), and SHV-70 (1 strain). The widespread presence of extended-spectrum β-lactamases in E. cloacae isolated from the southwest of China was likely due to the dissemination of resistance plasmids with the predominant genotype of blaCTX-M-22.</description><identifier>ISSN: 1344-6304</identifier><identifier>EISSN: 1884-2836</identifier><identifier>DOI: 10.7883/yoken.JJID.2008.286</identifier><identifier>PMID: 18653970</identifier><language>eng</language><publisher>Japan: National Institute of Infectious Diseases</publisher><subject>Anti-Bacterial Agents - pharmacology ; Aztreonam - pharmacology ; beta-Lactam Resistance - genetics ; beta-Lactamases - biosynthesis ; beta-Lactamases - genetics ; Cephalosporins - pharmacology ; China ; Conjugation, Genetic ; Enterobacter cloacae - drug effects ; Enterobacter cloacae - enzymology ; Enterobacter cloacae - genetics ; Enterobacter cloacae - isolation & purification ; Enterobacteriaceae Infections - microbiology ; Hospitals, Teaching ; Humans ; Isoelectric Focusing ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Plasmids ; Polymerase Chain Reaction ; Sequence Analysis, DNA</subject><ispartof>Japanese Journal of Infectious Diseases, 2008/07/28, Vol.61(4), pp.286-289</ispartof><rights>2008 Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-d15a4343be74760473db7c29d628f5cfe65946946bceda542cf72d3e5e41a50d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18653970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Gang</creatorcontrib><creatorcontrib>Ling, Bao-Dong</creatorcontrib><creatorcontrib>Zeng, Yun</creatorcontrib><creatorcontrib>Lin, Li</creatorcontrib><creatorcontrib>Xie, Yong-En</creatorcontrib><creatorcontrib>Lei, Jun</creatorcontrib><title>Molecular Characterization of Extended-Spectrum β-Lactamases Produced by Clinical Isolates of Enterobacter cloacae from a Teaching Hospital in China</title><title>Japanese Journal of Infectious Diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>Of 59 clinical isolates of Enterobacter cloacae from a teaching hospital in Sichuan, China, 18 isolates were shown to be resistant to oxyimino cephalosporins and aztreonam. Enterobacterial repetitive consensus PCR revealed that these isolates comprised 7 distinct genotypes. The presence of plasmids in the 18 clinical isolates was revealed by conjugational transfer of plasmids from E. cloacae to Escherichia coli with the further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing and β-lactamase isoelectric focusing indicated that the plasmids encoded blaSHV, blaCTX-M and/or blaTEM genes, including genes for CTX-M-22 (13 strains), TEM-1 (12 strains), TEM-29 (1 strain), TEM-141 (1 strain), TEM-157 (1 strain), SHV-5 (1 strain), SHV-12 (1 strain), and SHV-70 (1 strain). The widespread presence of extended-spectrum β-lactamases in E. cloacae isolated from the southwest of China was likely due to the dissemination of resistance plasmids with the predominant genotype of blaCTX-M-22.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Aztreonam - pharmacology</subject><subject>beta-Lactam Resistance - genetics</subject><subject>beta-Lactamases - biosynthesis</subject><subject>beta-Lactamases - genetics</subject><subject>Cephalosporins - pharmacology</subject><subject>China</subject><subject>Conjugation, Genetic</subject><subject>Enterobacter cloacae - drug effects</subject><subject>Enterobacter cloacae - enzymology</subject><subject>Enterobacter cloacae - genetics</subject><subject>Enterobacter cloacae - isolation & purification</subject><subject>Enterobacteriaceae Infections - microbiology</subject><subject>Hospitals, Teaching</subject><subject>Humans</subject><subject>Isoelectric Focusing</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><issn>1344-6304</issn><issn>1884-2836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkd9qFDEUhwdRbK0-gSC58m7WzOTPzF7KWu2WFQXrdTiTnGlTM8maZMD1PfoifRCfyWx3qQghOZDvfBzOr6peN3TR9T17tws_0C8uL9cfFi2l_aLt5ZPqtOl7Xrc9k09LzTivJaP8pHqR0i2lrRANfV6dNL0UbNnR0-ruc3CoZweRrG4ggs4Y7W_INngSRnL-K6M3aOpvW9Q5zhP5c19vCgUTJEzkawxm1mjIsCMrZ73V4Mg6BQe5_O4FvgjD8OAl2gXQgGSMYSJArhD0jfXX5CKkrc2l0_oyhfXwsno2gkv46vieVd8_nl-tLurNl0_r1ftNrQXluTaNAM44G7DjnaS8Y2bodLs0su1HoUeUYsllOUMZEQRv9di1hqFA3oCghp1Vbw_ebQw_Z0xZTTZpdA48hjkpuSwbpJ0oIDuAOoaUIo5qG-0EcacaqvZpqIc01D4NtU9DlTRK15ujfh4mNP96jusvwPoA3KYM1_gIQMxWOzxKZaP4_vpP_sjoEptCz_4C6IClOQ</recordid><startdate>20080728</startdate><enddate>20080728</enddate><creator>Liu, Gang</creator><creator>Ling, Bao-Dong</creator><creator>Zeng, Yun</creator><creator>Lin, Li</creator><creator>Xie, Yong-En</creator><creator>Lei, Jun</creator><general>National Institute of Infectious Diseases</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080728</creationdate><title>Molecular Characterization of Extended-Spectrum β-Lactamases Produced by Clinical Isolates of Enterobacter cloacae from a Teaching Hospital in China</title><author>Liu, Gang ; Ling, Bao-Dong ; Zeng, Yun ; Lin, Li ; Xie, Yong-En ; Lei, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-d15a4343be74760473db7c29d628f5cfe65946946bceda542cf72d3e5e41a50d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Aztreonam - pharmacology</topic><topic>beta-Lactam Resistance - genetics</topic><topic>beta-Lactamases - biosynthesis</topic><topic>beta-Lactamases - genetics</topic><topic>Cephalosporins - pharmacology</topic><topic>China</topic><topic>Conjugation, Genetic</topic><topic>Enterobacter cloacae - drug effects</topic><topic>Enterobacter cloacae - enzymology</topic><topic>Enterobacter cloacae - genetics</topic><topic>Enterobacter cloacae - isolation & purification</topic><topic>Enterobacteriaceae Infections - microbiology</topic><topic>Hospitals, Teaching</topic><topic>Humans</topic><topic>Isoelectric Focusing</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Gang</creatorcontrib><creatorcontrib>Ling, Bao-Dong</creatorcontrib><creatorcontrib>Zeng, Yun</creatorcontrib><creatorcontrib>Lin, Li</creatorcontrib><creatorcontrib>Xie, Yong-En</creatorcontrib><creatorcontrib>Lei, Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese Journal of Infectious Diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Gang</au><au>Ling, Bao-Dong</au><au>Zeng, Yun</au><au>Lin, Li</au><au>Xie, Yong-En</au><au>Lei, Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Characterization of Extended-Spectrum β-Lactamases Produced by Clinical Isolates of Enterobacter cloacae from a Teaching Hospital in China</atitle><jtitle>Japanese Journal of Infectious Diseases</jtitle><addtitle>Jpn J Infect Dis</addtitle><date>2008-07-28</date><risdate>2008</risdate><volume>61</volume><issue>4</issue><spage>286</spage><epage>289</epage><pages>286-289</pages><artnum>JJID.2008.286</artnum><issn>1344-6304</issn><eissn>1884-2836</eissn><abstract>Of 59 clinical isolates of Enterobacter cloacae from a teaching hospital in Sichuan, China, 18 isolates were shown to be resistant to oxyimino cephalosporins and aztreonam. Enterobacterial repetitive consensus PCR revealed that these isolates comprised 7 distinct genotypes. The presence of plasmids in the 18 clinical isolates was revealed by conjugational transfer of plasmids from E. cloacae to Escherichia coli with the further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing and β-lactamase isoelectric focusing indicated that the plasmids encoded blaSHV, blaCTX-M and/or blaTEM genes, including genes for CTX-M-22 (13 strains), TEM-1 (12 strains), TEM-29 (1 strain), TEM-141 (1 strain), TEM-157 (1 strain), SHV-5 (1 strain), SHV-12 (1 strain), and SHV-70 (1 strain). The widespread presence of extended-spectrum β-lactamases in E. cloacae isolated from the southwest of China was likely due to the dissemination of resistance plasmids with the predominant genotype of blaCTX-M-22.</abstract><cop>Japan</cop><pub>National Institute of Infectious Diseases</pub><pmid>18653970</pmid><doi>10.7883/yoken.JJID.2008.286</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals |
| subjects | Anti-Bacterial Agents - pharmacology Aztreonam - pharmacology beta-Lactam Resistance - genetics beta-Lactamases - biosynthesis beta-Lactamases - genetics Cephalosporins - pharmacology China Conjugation, Genetic Enterobacter cloacae - drug effects Enterobacter cloacae - enzymology Enterobacter cloacae - genetics Enterobacter cloacae - isolation & purification Enterobacteriaceae Infections - microbiology Hospitals, Teaching Humans Isoelectric Focusing Microbial Sensitivity Tests Molecular Sequence Data Plasmids Polymerase Chain Reaction Sequence Analysis, DNA |
| title | Molecular Characterization of Extended-Spectrum β-Lactamases Produced by Clinical Isolates of Enterobacter cloacae from a Teaching Hospital in China |
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