Usefulness of Statistic Experimental Designs in Enzymology: Example with Recombinant hCYP3A4 and 1A2

First, the effects of 10 incubation factors were screened altogether on nifedipine dehydrogenase (NIF) and methoxyresorufin O-deethylase (MROD) activities catalyzed by recombinant human CYP3A4 and 1A2, respectively. Using a statistic experimental design, only 36 assays were needed to be exhaustive....

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Veröffentlicht in:	Analytical biochemistry 1999-12, Vol.276 (1), p.18-26
Hauptverfasser:	Bournique, B., Petry, M., Gousset, G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biometry CYP1A2 CYP3A4 Cytochrome P-450 CYP1A2 - genetics Cytochrome P-450 CYP1A2 - metabolism Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism drug metabolism enzyme experimental design heterologous expression Humans In Vitro Techniques Kinetics Magnesium - pharmacology membrane Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism modeling Models, Biological MROD nifedipine optimization Osmolar Concentration Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae - genetics Substrate Specificity yeast
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