Increased c-Fos/activator protein-1 confers resistance against anergy induction on antigen-specific T cell

We have studied the contribution of c-Fos/activator protein-1 (AP-1) to antigen-specific T cell response with reference to T cell anergy by increasing c-Fos/AP-1 in vivo and in vitro. First, after injection of a high dose of staphylococcus enterotoxin B (SEB), clonal deletion of SEB-reactive Vβ8+ CD...

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Veröffentlicht in:International immunology 1999-12, Vol.11 (12), p.1873-1880
Hauptverfasser: Kawasaki1, Hiroki, Nakata, Yukiko, Suzuki, Gen, Chihara, Kazuo, Tokuhisa, Takeshi, Shiozawa, Shunichi
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Sprache:eng
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Zusammenfassung:We have studied the contribution of c-Fos/activator protein-1 (AP-1) to antigen-specific T cell response with reference to T cell anergy by increasing c-Fos/AP-1 in vivo and in vitro. First, after injection of a high dose of staphylococcus enterotoxin B (SEB), clonal deletion of SEB-reactive Vβ8+ CD4 T cells occurred both in control B6 and H2-c-fos transgenic (fos) mice, whereas proliferation of T cells against SEB was profoundly depressed in B6 but not in fos mice. Second, the keyhole limpet hemocyanin-specific CD4 Th1 cell clone produced decreasing amounts of IL-2 in response to increasing amounts of concanavalin A (Con A) in vitro, whereas the decrease was less significant in the Th1 clones stably transfected with c-fos gene. Electrophoretic mobility shift assay with nuclear protein from the transformants showed that overexpression of the c-fos gene compensated the amounts of AP-1 in the nuclei of Con A-treated Th1 clones. Thus, increased c-Fos/AP-1 confers resistance against anergy induction on antigen-specific T cells.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/11.12.1873