Live Bartonella henselae enhances endothelial cell proliferation without direct contact
The proliferation of human umbilical vein endothelial cells (HUVECs) cocultivated with live B. henselae was enhanced in a bacterial dose-dependent manner, and the stimulatory effect was specific to vascular endothelial cells. The inactivation of B. henselae by UV or heat treatment abolished its stim...
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| Veröffentlicht in: | Microbial pathogenesis 1999-12, Vol.27 (6), p.419-427 |
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| container_title | Microbial pathogenesis |
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| creator | Maeno, Nobuaki Oda, Hiroshi Yoshiie, Kiyotaka Wahid, Mohammad Rezwanul Fujimura, Tsuyoshi Matayoshi, Seiken |
| description | The proliferation of human umbilical vein endothelial cells (HUVECs) cocultivated with live B. henselae was enhanced in a bacterial dose-dependent manner, and the stimulatory effect was specific to vascular endothelial cells. The inactivation of B. henselae by UV or heat treatment abolished its stimulatory activity, suggesting that live bacteria is necessary for the growth stimulation effect. To investigate the role of direct contact, live B. henselae were separated from HUVECs by a filter membrane (Millicell-CM insert). Even under this condition, an enhanced proliferation of HUVECs was observed. However, no morphological changes in the HUVECs were apparent compared to the B. henselae -infected cells. Furthermore, we isolated a nonpiliated strain of B. henselae that is unable to attach to and enter into endothelial cells. The nonpiliated strain possessed the ability to stimulate the proliferation of cocultivated HUVECs the same as the piliated strain. Moreover, the culture supernatants of B. henselae were also able to induce HUVEC proliferation. Our results indicate that the stimulation of HUVEC proliferation by B. henselae is mediated by soluble factor(s) secreted from the bacteria. |
| doi_str_mv | 10.1006/mpat.1999.0315 |
| format | Article |
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The inactivation of B. henselae by UV or heat treatment abolished its stimulatory activity, suggesting that live bacteria is necessary for the growth stimulation effect. To investigate the role of direct contact, live B. henselae were separated from HUVECs by a filter membrane (Millicell-CM insert). Even under this condition, an enhanced proliferation of HUVECs was observed. However, no morphological changes in the HUVECs were apparent compared to the B. henselae -infected cells. Furthermore, we isolated a nonpiliated strain of B. henselae that is unable to attach to and enter into endothelial cells. The nonpiliated strain possessed the ability to stimulate the proliferation of cocultivated HUVECs the same as the piliated strain. Moreover, the culture supernatants of B. henselae were also able to induce HUVEC proliferation. Our results indicate that the stimulation of HUVEC proliferation by B. henselae is mediated by soluble factor(s) secreted from the bacteria.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1006/mpat.1999.0315</identifier><identifier>PMID: 10588914</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Angiomatosis, Bacillary - microbiology ; Bacteriology ; Bartonella henselae ; Bartonella henselae - physiology ; Bartonella henselae - radiation effects ; Bartonella henselae, endothelial cell, direct contact, pili, culture supernatant ; Biological and medical sciences ; Cell Division ; Cells, Cultured ; Coculture Techniques ; Endothelium, Vascular - cytology ; Endothelium, Vascular - microbiology ; Fimbriae, Bacterial - physiology ; Fundamental and applied biological sciences. Psychology ; Hot Temperature ; Humans ; Microbiology ; Neovascularization, Pathologic - physiopathology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Ultraviolet Rays ; Umbilical Veins</subject><ispartof>Microbial pathogenesis, 1999-12, Vol.27 (6), p.419-427</ispartof><rights>1999 Academic Press</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/mpat.1999.0315$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1251225$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10588914$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maeno, Nobuaki</creatorcontrib><creatorcontrib>Oda, Hiroshi</creatorcontrib><creatorcontrib>Yoshiie, Kiyotaka</creatorcontrib><creatorcontrib>Wahid, Mohammad Rezwanul</creatorcontrib><creatorcontrib>Fujimura, Tsuyoshi</creatorcontrib><creatorcontrib>Matayoshi, Seiken</creatorcontrib><title>Live Bartonella henselae enhances endothelial cell proliferation without direct contact</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>The proliferation of human umbilical vein endothelial cells (HUVECs) cocultivated with live B. henselae was enhanced in a bacterial dose-dependent manner, and the stimulatory effect was specific to vascular endothelial cells. The inactivation of B. henselae by UV or heat treatment abolished its stimulatory activity, suggesting that live bacteria is necessary for the growth stimulation effect. To investigate the role of direct contact, live B. henselae were separated from HUVECs by a filter membrane (Millicell-CM insert). Even under this condition, an enhanced proliferation of HUVECs was observed. However, no morphological changes in the HUVECs were apparent compared to the B. henselae -infected cells. Furthermore, we isolated a nonpiliated strain of B. henselae that is unable to attach to and enter into endothelial cells. The nonpiliated strain possessed the ability to stimulate the proliferation of cocultivated HUVECs the same as the piliated strain. Moreover, the culture supernatants of B. henselae were also able to induce HUVEC proliferation. Our results indicate that the stimulation of HUVEC proliferation by B. henselae is mediated by soluble factor(s) secreted from the bacteria.</description><subject>Angiomatosis, Bacillary - microbiology</subject><subject>Bacteriology</subject><subject>Bartonella henselae</subject><subject>Bartonella henselae - physiology</subject><subject>Bartonella henselae - radiation effects</subject><subject>Bartonella henselae, endothelial cell, direct contact, pili, culture supernatant</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - microbiology</subject><subject>Fimbriae, Bacterial - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Neovascularization, Pathologic - physiopathology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Ultraviolet Rays</subject><subject>Umbilical Veins</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTFv2zAQRomiQeOmXTsWGopuco-kKJFjEjRNAANdEmQkTuQRZiFLrkgnyL8PBTvo2OlueDh89z7GvnBYc4D2x26Pec2NMWuQXL1jKw6mrbkA_Z6tQGtRN8DhnH1M6Q8AmEaaD-ycg9La8GbFHjfxiaornPM00jBgtaUx0YBU0bjF0VEqi5_yloaIQ-UKU-3naYiBZsxxGqvnmLfTIVc-zuRy5aYxo8uf2FnAIdHn07xgDzc_769v683vX3fXl5uaJG9zbbwKIhjptfc9al6CeY9CQAPBy143CE4F6ZwPvUKlul50OnTYS9_zVkp5wb4f75ZQfw-Ust3FtKTEkaZDsq2RsuPQ_BfkXdNw04oCfj2Bh35H3u7nuMP5xb45K8C3E4DJ4RDmoimmf5xQXAhVMH3EqLz_FGm2yUUqRo-irJ9iuWmXFu3Sol1atEuL8hXgao_D</recordid><startdate>19991201</startdate><enddate>19991201</enddate><creator>Maeno, Nobuaki</creator><creator>Oda, Hiroshi</creator><creator>Yoshiie, Kiyotaka</creator><creator>Wahid, Mohammad Rezwanul</creator><creator>Fujimura, Tsuyoshi</creator><creator>Matayoshi, Seiken</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19991201</creationdate><title>Live Bartonella henselae enhances endothelial cell proliferation without direct contact</title><author>Maeno, Nobuaki ; Oda, Hiroshi ; Yoshiie, Kiyotaka ; Wahid, Mohammad Rezwanul ; Fujimura, Tsuyoshi ; Matayoshi, Seiken</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e316t-9d5f2f93d8ddba81105dda22040fd3b84a0c5f3ccdfb5a557b278f7ab3db16333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Angiomatosis, Bacillary - microbiology</topic><topic>Bacteriology</topic><topic>Bartonella henselae</topic><topic>Bartonella henselae - physiology</topic><topic>Bartonella henselae - radiation effects</topic><topic>Bartonella henselae, endothelial cell, direct contact, pili, culture supernatant</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Coculture Techniques</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - microbiology</topic><topic>Fimbriae, Bacterial - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Neovascularization, Pathologic - physiopathology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Ultraviolet Rays</topic><topic>Umbilical Veins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maeno, Nobuaki</creatorcontrib><creatorcontrib>Oda, Hiroshi</creatorcontrib><creatorcontrib>Yoshiie, Kiyotaka</creatorcontrib><creatorcontrib>Wahid, Mohammad Rezwanul</creatorcontrib><creatorcontrib>Fujimura, Tsuyoshi</creatorcontrib><creatorcontrib>Matayoshi, Seiken</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maeno, Nobuaki</au><au>Oda, Hiroshi</au><au>Yoshiie, Kiyotaka</au><au>Wahid, Mohammad Rezwanul</au><au>Fujimura, Tsuyoshi</au><au>Matayoshi, Seiken</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Live Bartonella henselae enhances endothelial cell proliferation without direct contact</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>1999-12-01</date><risdate>1999</risdate><volume>27</volume><issue>6</issue><spage>419</spage><epage>427</epage><pages>419-427</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>The proliferation of human umbilical vein endothelial cells (HUVECs) cocultivated with live B. henselae was enhanced in a bacterial dose-dependent manner, and the stimulatory effect was specific to vascular endothelial cells. The inactivation of B. henselae by UV or heat treatment abolished its stimulatory activity, suggesting that live bacteria is necessary for the growth stimulation effect. To investigate the role of direct contact, live B. henselae were separated from HUVECs by a filter membrane (Millicell-CM insert). Even under this condition, an enhanced proliferation of HUVECs was observed. However, no morphological changes in the HUVECs were apparent compared to the B. henselae -infected cells. Furthermore, we isolated a nonpiliated strain of B. henselae that is unable to attach to and enter into endothelial cells. The nonpiliated strain possessed the ability to stimulate the proliferation of cocultivated HUVECs the same as the piliated strain. Moreover, the culture supernatants of B. henselae were also able to induce HUVEC proliferation. 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| subjects | Angiomatosis, Bacillary - microbiology Bacteriology Bartonella henselae Bartonella henselae - physiology Bartonella henselae - radiation effects Bartonella henselae, endothelial cell, direct contact, pili, culture supernatant Biological and medical sciences Cell Division Cells, Cultured Coculture Techniques Endothelium, Vascular - cytology Endothelium, Vascular - microbiology Fimbriae, Bacterial - physiology Fundamental and applied biological sciences. Psychology Hot Temperature Humans Microbiology Neovascularization, Pathologic - physiopathology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Ultraviolet Rays Umbilical Veins |
| title | Live Bartonella henselae enhances endothelial cell proliferation without direct contact |
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