Correlation between virulence factors and in vitro biofilm formation by Escherichia coli strains
The ability of 15 Escherichia coli strains to form biofilms on polystirene plates was studied. The strains were serotyped, and their phenotypic expression of surface virulence factors (VFs), and antibiotic susceptibility was also determined. Moreover, 30 VFs-associated genes were analysed, including...
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| Veröffentlicht in: | Microbial pathogenesis 2008-08, Vol.45 (2), p.86-91 |
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| creator | Naves, Plínio del Prado, Gema Huelves, Lorena Gracia, Matilde Ruiz, Vicente Blanco, Jorge Dahbi, Ghizlane Blanco, Miguel del Carmen Ponte, María Soriano, Francisco |
| description | The ability of 15
Escherichia coli strains to form biofilms on polystirene plates was studied. The strains were serotyped, and their phenotypic expression of surface virulence factors (VFs), and antibiotic susceptibility was also determined. Moreover, 30 VFs-associated genes were analysed, including 15 adhesins (
papC,
papG and its three alleles,
sfa/focDE,
sfaS,
focG,
afa/draBC,
iha,
bmaE,
gafD,
nfaE,
fimH, fimAvMT78,
agn43, F9 fimbriae and type 3 fimbriae-encoding gene clusters), four toxins (
hlyA, cnf1, sat and
tsh), four siderophore (
iron, fyuA, iutA and
iucD), five proctetins/invasion-encoding genes (
kpsM II,
kpsMT III, K1
kps variant-
neuC, traT and
ibeA), and the pathogenicity island
malX and
cvaC. Morphological appearance and thickness of biofilms of two strong and three weak biofilm producers were also studied by confocal laser scanning microscopy (CLSM). Seven strains were classified as strong biofilm producers and the remaining eight strains were regarded as weak biofilm producers.
Mannose-resistant haemagglutination was the only phenotypically expressed surface virulence factor more frequently found in the strong biofilm group. Five virulence-associated genes were more common (
p
<
0.05) in strong biofilm producers:
papC and
papG alleles,
sfa/focDE,
focG,
hlyA and
cnf1. CLSM images showed irregular biofilms with projections at the top mainly in strong biofilm. |
| doi_str_mv | 10.1016/j.micpath.2008.03.003 |
| format | Article |
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Escherichia coli strains to form biofilms on polystirene plates was studied. The strains were serotyped, and their phenotypic expression of surface virulence factors (VFs), and antibiotic susceptibility was also determined. Moreover, 30 VFs-associated genes were analysed, including 15 adhesins (
papC,
papG and its three alleles,
sfa/focDE,
sfaS,
focG,
afa/draBC,
iha,
bmaE,
gafD,
nfaE,
fimH, fimAvMT78,
agn43, F9 fimbriae and type 3 fimbriae-encoding gene clusters), four toxins (
hlyA, cnf1, sat and
tsh), four siderophore (
iron, fyuA, iutA and
iucD), five proctetins/invasion-encoding genes (
kpsM II,
kpsMT III, K1
kps variant-
neuC, traT and
ibeA), and the pathogenicity island
malX and
cvaC. Morphological appearance and thickness of biofilms of two strong and three weak biofilm producers were also studied by confocal laser scanning microscopy (CLSM). Seven strains were classified as strong biofilm producers and the remaining eight strains were regarded as weak biofilm producers.
Mannose-resistant haemagglutination was the only phenotypically expressed surface virulence factor more frequently found in the strong biofilm group. Five virulence-associated genes were more common (
p
<
0.05) in strong biofilm producers:
papC and
papG alleles,
sfa/focDE,
focG,
hlyA and
cnf1. CLSM images showed irregular biofilms with projections at the top mainly in strong biofilm.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2008.03.003</identifier><identifier>PMID: 18486439</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Oxford: Elsevier India Pvt Ltd</publisher><subject>Bacterial Adhesion - physiology ; Bacteriology ; Biofilm ; Biofilms - growth & development ; Biological and medical sciences ; Escherichia coli ; Escherichia coli - classification ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli - pathogenicity ; Escherichia coli Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Microscopy, Confocal ; Miscellaneous ; Virulence - genetics ; Virulence - physiology ; Virulence factors ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>Microbial pathogenesis, 2008-08, Vol.45 (2), p.86-91</ispartof><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-61c1317930a298c7f4921086e9432f67d4af0022bf67580ccd5db0ba07a867563</citedby><cites>FETCH-LOGICAL-c424t-61c1317930a298c7f4921086e9432f67d4af0022bf67580ccd5db0ba07a867563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0882401008000363$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20553673$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18486439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naves, Plínio</creatorcontrib><creatorcontrib>del Prado, Gema</creatorcontrib><creatorcontrib>Huelves, Lorena</creatorcontrib><creatorcontrib>Gracia, Matilde</creatorcontrib><creatorcontrib>Ruiz, Vicente</creatorcontrib><creatorcontrib>Blanco, Jorge</creatorcontrib><creatorcontrib>Dahbi, Ghizlane</creatorcontrib><creatorcontrib>Blanco, Miguel</creatorcontrib><creatorcontrib>del Carmen Ponte, María</creatorcontrib><creatorcontrib>Soriano, Francisco</creatorcontrib><title>Correlation between virulence factors and in vitro biofilm formation by Escherichia coli strains</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>The ability of 15
Escherichia coli strains to form biofilms on polystirene plates was studied. The strains were serotyped, and their phenotypic expression of surface virulence factors (VFs), and antibiotic susceptibility was also determined. Moreover, 30 VFs-associated genes were analysed, including 15 adhesins (
papC,
papG and its three alleles,
sfa/focDE,
sfaS,
focG,
afa/draBC,
iha,
bmaE,
gafD,
nfaE,
fimH, fimAvMT78,
agn43, F9 fimbriae and type 3 fimbriae-encoding gene clusters), four toxins (
hlyA, cnf1, sat and
tsh), four siderophore (
iron, fyuA, iutA and
iucD), five proctetins/invasion-encoding genes (
kpsM II,
kpsMT III, K1
kps variant-
neuC, traT and
ibeA), and the pathogenicity island
malX and
cvaC. Morphological appearance and thickness of biofilms of two strong and three weak biofilm producers were also studied by confocal laser scanning microscopy (CLSM). Seven strains were classified as strong biofilm producers and the remaining eight strains were regarded as weak biofilm producers.
Mannose-resistant haemagglutination was the only phenotypically expressed surface virulence factor more frequently found in the strong biofilm group. Five virulence-associated genes were more common (
p
<
0.05) in strong biofilm producers:
papC and
papG alleles,
sfa/focDE,
focG,
hlyA and
cnf1. CLSM images showed irregular biofilms with projections at the top mainly in strong biofilm.</description><subject>Bacterial Adhesion - physiology</subject><subject>Bacteriology</subject><subject>Biofilm</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Escherichia coli</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Microscopy, Confocal</subject><subject>Miscellaneous</subject><subject>Virulence - genetics</subject><subject>Virulence - physiology</subject><subject>Virulence factors</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EapfCTwD5AreEsZ04zgmhVaGVKnGhZ9dxbO2sknixvUX993i7ERx78sj-nufpPUI-MKgZMPllX89oDybvag6gahA1gHhFNgx6WTEO6jXZgFK8aoDBJXmb0h4A-kb0F-SSqUbJMm7IwzbE6CaTMSx0cPmPcwt9xHic3GId9cbmEBM1y0jx9JBjoAMGj9NMfYjzKnyi18nuXES7Q0NtmJCmHA0u6R15482U3Pv1vCL3369_bW-qu58_brff7irb8CZXklkmWNcLMLxXtvNNzxko6Ypj7mU3NsYDcD6UuVVg7diOAwwGOqPKjRRX5PP530MMv48uZT1jsm6azOLCMWnZC66kaF4ES3QdtJ0oYHsGbQwpRef1IeJs4pNmoE8d6L1eO9CnDjQIXToouo_rguMwu_G_ag29AJ9WwCRrJh_NYjH94zi0rZDPBr6eOVdye0QXdbJ4qmXE6GzWY8AXrPwFOCynpw</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Naves, Plínio</creator><creator>del Prado, Gema</creator><creator>Huelves, Lorena</creator><creator>Gracia, Matilde</creator><creator>Ruiz, Vicente</creator><creator>Blanco, Jorge</creator><creator>Dahbi, Ghizlane</creator><creator>Blanco, Miguel</creator><creator>del Carmen Ponte, María</creator><creator>Soriano, Francisco</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20080801</creationdate><title>Correlation between virulence factors and in vitro biofilm formation by Escherichia coli strains</title><author>Naves, Plínio ; del Prado, Gema ; Huelves, Lorena ; Gracia, Matilde ; Ruiz, Vicente ; Blanco, Jorge ; Dahbi, Ghizlane ; Blanco, Miguel ; del Carmen Ponte, María ; Soriano, Francisco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-61c1317930a298c7f4921086e9432f67d4af0022bf67580ccd5db0ba07a867563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bacterial Adhesion - physiology</topic><topic>Bacteriology</topic><topic>Biofilm</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Escherichia coli</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Microscopy, Confocal</topic><topic>Miscellaneous</topic><topic>Virulence - genetics</topic><topic>Virulence - physiology</topic><topic>Virulence factors</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naves, Plínio</creatorcontrib><creatorcontrib>del Prado, Gema</creatorcontrib><creatorcontrib>Huelves, Lorena</creatorcontrib><creatorcontrib>Gracia, Matilde</creatorcontrib><creatorcontrib>Ruiz, Vicente</creatorcontrib><creatorcontrib>Blanco, Jorge</creatorcontrib><creatorcontrib>Dahbi, Ghizlane</creatorcontrib><creatorcontrib>Blanco, Miguel</creatorcontrib><creatorcontrib>del Carmen Ponte, María</creatorcontrib><creatorcontrib>Soriano, Francisco</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naves, Plínio</au><au>del Prado, Gema</au><au>Huelves, Lorena</au><au>Gracia, Matilde</au><au>Ruiz, Vicente</au><au>Blanco, Jorge</au><au>Dahbi, Ghizlane</au><au>Blanco, Miguel</au><au>del Carmen Ponte, María</au><au>Soriano, Francisco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation between virulence factors and in vitro biofilm formation by Escherichia coli strains</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>45</volume><issue>2</issue><spage>86</spage><epage>91</epage><pages>86-91</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>The ability of 15
Escherichia coli strains to form biofilms on polystirene plates was studied. The strains were serotyped, and their phenotypic expression of surface virulence factors (VFs), and antibiotic susceptibility was also determined. Moreover, 30 VFs-associated genes were analysed, including 15 adhesins (
papC,
papG and its three alleles,
sfa/focDE,
sfaS,
focG,
afa/draBC,
iha,
bmaE,
gafD,
nfaE,
fimH, fimAvMT78,
agn43, F9 fimbriae and type 3 fimbriae-encoding gene clusters), four toxins (
hlyA, cnf1, sat and
tsh), four siderophore (
iron, fyuA, iutA and
iucD), five proctetins/invasion-encoding genes (
kpsM II,
kpsMT III, K1
kps variant-
neuC, traT and
ibeA), and the pathogenicity island
malX and
cvaC. Morphological appearance and thickness of biofilms of two strong and three weak biofilm producers were also studied by confocal laser scanning microscopy (CLSM). Seven strains were classified as strong biofilm producers and the remaining eight strains were regarded as weak biofilm producers.
Mannose-resistant haemagglutination was the only phenotypically expressed surface virulence factor more frequently found in the strong biofilm group. Five virulence-associated genes were more common (
p
<
0.05) in strong biofilm producers:
papC and
papG alleles,
sfa/focDE,
focG,
hlyA and
cnf1. CLSM images showed irregular biofilms with projections at the top mainly in strong biofilm.</abstract><cop>Oxford</cop><pub>Elsevier India Pvt Ltd</pub><pmid>18486439</pmid><doi>10.1016/j.micpath.2008.03.003</doi><tpages>6</tpages></addata></record> |
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| ispartof | Microbial pathogenesis, 2008-08, Vol.45 (2), p.86-91 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Bacterial Adhesion - physiology Bacteriology Biofilm Biofilms - growth & development Biological and medical sciences Escherichia coli Escherichia coli - classification Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli - pathogenicity Escherichia coli Proteins - metabolism Fundamental and applied biological sciences. Psychology Microbiology Microscopy, Confocal Miscellaneous Virulence - genetics Virulence - physiology Virulence factors Virulence Factors - genetics Virulence Factors - metabolism |
| title | Correlation between virulence factors and in vitro biofilm formation by Escherichia coli strains |
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