Sevoflurane and the Feto-Placental Vasculature: The Role of Nitric Oxide and Vasoactive Eicosanoids
The effects and mechanisms of action of volatile anesthetics on the feto-placental vasculature are not known. We aimed to quantify the vasoactive effects of sevoflurane and determine the role of nitric oxide (NO) and of vasoactive eicosanoids in mediating these effects in isolated human chorionic pl...
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| Veröffentlicht in: | Anesthesia and analgesia 2008-07, Vol.107 (1), p.171-177 |
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| creator | Farragher, Rachel Maharaj, Chrisen H. Higgins, Brendan D. Crowe, Sharon Burke, Padvaic Laffey, Christopher D. Flynn, Noel M. Laffey, John G. |
| description | The effects and mechanisms of action of volatile anesthetics on the feto-placental vasculature are not known. We aimed to quantify the vasoactive effects of sevoflurane and determine the role of nitric oxide (NO) and of vasoactive eicosanoids in mediating these effects in isolated human chorionic plate arterial rings.
Quadruplicate ex vivo human chorionic plate arterial rings were used in all studies. Series 1 quantified the vasodilation produced by sevoflurane in rings preconstricted with the thromboxane analog U46619. Series 2A-C examined the role of NO in sevoflurane-mediated vasodilation. In separate experiments, we examined the potential for the nonspecific NO inhibitors, L-NAME, L-nMMA, and the inactive D-NAME, to modulate the vasodilation produced by sevoflurane. Series 2D determined whether sevoflurane altered vascular smooth muscle sensitivity to exogenous NO. Series 3A-D examined the role of vasoactive eicosanoids in sevoflurane-mediated vasodilation. In separate experimental series, we examined whether the nonspecific cyclooxygenase inhibitor, indomethacin, or the 5-lipoxygenase inhibitor, nordihydroguaiaretic acid, modulated sevoflurane-mediated vasodilation.
Sevoflurane produced dose-dependent vasodilation of preconstricted chorionic plate arterial rings, with mean ring vasodilation increasing from 15 +/- 7% at 2% sevoflurane to 67 +/- 17% (mean +/- sd) at 8% sevoflurane. Blockade of NO synthase did not attenuate the vasodilator effects of sevoflurane. Sevoflurane did not alter smooth muscle sensitivity to NO. Indomethacin augmented sevoflurane vasodilation at 10(-5) M, but not at 10(-6) M. Conversely, nordihydroguaiaretic acid attenuated sevoflurane-mediated vasodilation at 3 x 10(-6) M but not at 3 x 10(-7) M.
Sevoflurane was a vasodilator in the feto-placental vasculature in this in vitro model. Sevoflurane-mediated vasodilation is NO and cyclooxygenase-independent and appears to be mediated in part via a lipoxygenase generated vasodilator eicosanoid. |
| doi_str_mv | 10.1213/ane.0b013e318173465e |
| format | Article |
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Quadruplicate ex vivo human chorionic plate arterial rings were used in all studies. Series 1 quantified the vasodilation produced by sevoflurane in rings preconstricted with the thromboxane analog U46619. Series 2A-C examined the role of NO in sevoflurane-mediated vasodilation. In separate experiments, we examined the potential for the nonspecific NO inhibitors, L-NAME, L-nMMA, and the inactive D-NAME, to modulate the vasodilation produced by sevoflurane. Series 2D determined whether sevoflurane altered vascular smooth muscle sensitivity to exogenous NO. Series 3A-D examined the role of vasoactive eicosanoids in sevoflurane-mediated vasodilation. In separate experimental series, we examined whether the nonspecific cyclooxygenase inhibitor, indomethacin, or the 5-lipoxygenase inhibitor, nordihydroguaiaretic acid, modulated sevoflurane-mediated vasodilation.
Sevoflurane produced dose-dependent vasodilation of preconstricted chorionic plate arterial rings, with mean ring vasodilation increasing from 15 +/- 7% at 2% sevoflurane to 67 +/- 17% (mean +/- sd) at 8% sevoflurane. Blockade of NO synthase did not attenuate the vasodilator effects of sevoflurane. Sevoflurane did not alter smooth muscle sensitivity to NO. Indomethacin augmented sevoflurane vasodilation at 10(-5) M, but not at 10(-6) M. Conversely, nordihydroguaiaretic acid attenuated sevoflurane-mediated vasodilation at 3 x 10(-6) M but not at 3 x 10(-7) M.
Sevoflurane was a vasodilator in the feto-placental vasculature in this in vitro model. Sevoflurane-mediated vasodilation is NO and cyclooxygenase-independent and appears to be mediated in part via a lipoxygenase generated vasodilator eicosanoid.</description><identifier>ISSN: 0003-2999</identifier><identifier>EISSN: 1526-7598</identifier><identifier>DOI: 10.1213/ane.0b013e318173465e</identifier><identifier>PMID: 18635485</identifier><identifier>CODEN: AACRAT</identifier><language>eng</language><publisher>Hagerstown, MD: International Anesthesia Research Society</publisher><subject>Anesthesia ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Anesthetics, Inhalation - pharmacology ; Biological and medical sciences ; Dose-Response Relationship, Drug ; Eicosanoids - physiology ; Female ; Fetus - blood supply ; Humans ; In Vitro Techniques ; Indomethacin - pharmacology ; Masoprocol - pharmacology ; Medical sciences ; Methyl Ethers - pharmacology ; Nitric Oxide - physiology ; Nitroprusside - pharmacology ; omega-N-Methylarginine - pharmacology ; Placenta - blood supply ; Pregnancy ; Vasodilation - drug effects</subject><ispartof>Anesthesia and analgesia, 2008-07, Vol.107 (1), p.171-177</ispartof><rights>International Anesthesia Research Society</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4269-daeba4af0d770e000818fd8cc330b0b071c8a13c7d6870d33aba862b72ad34f53</citedby><cites>FETCH-LOGICAL-c4269-daeba4af0d770e000818fd8cc330b0b071c8a13c7d6870d33aba862b72ad34f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&NEWS=n&CSC=Y&PAGE=fulltext&D=ovft&AN=00000539-200807000-00029$$EHTML$$P50$$Gwolterskluwer$$H</linktohtml><link.rule.ids>314,776,780,4595,27901,27902,65206</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20465788$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18635485$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farragher, Rachel</creatorcontrib><creatorcontrib>Maharaj, Chrisen H.</creatorcontrib><creatorcontrib>Higgins, Brendan D.</creatorcontrib><creatorcontrib>Crowe, Sharon</creatorcontrib><creatorcontrib>Burke, Padvaic</creatorcontrib><creatorcontrib>Laffey, Christopher D.</creatorcontrib><creatorcontrib>Flynn, Noel M.</creatorcontrib><creatorcontrib>Laffey, John G.</creatorcontrib><title>Sevoflurane and the Feto-Placental Vasculature: The Role of Nitric Oxide and Vasoactive Eicosanoids</title><title>Anesthesia and analgesia</title><addtitle>Anesth Analg</addtitle><description>The effects and mechanisms of action of volatile anesthetics on the feto-placental vasculature are not known. We aimed to quantify the vasoactive effects of sevoflurane and determine the role of nitric oxide (NO) and of vasoactive eicosanoids in mediating these effects in isolated human chorionic plate arterial rings.
Quadruplicate ex vivo human chorionic plate arterial rings were used in all studies. Series 1 quantified the vasodilation produced by sevoflurane in rings preconstricted with the thromboxane analog U46619. Series 2A-C examined the role of NO in sevoflurane-mediated vasodilation. In separate experiments, we examined the potential for the nonspecific NO inhibitors, L-NAME, L-nMMA, and the inactive D-NAME, to modulate the vasodilation produced by sevoflurane. Series 2D determined whether sevoflurane altered vascular smooth muscle sensitivity to exogenous NO. Series 3A-D examined the role of vasoactive eicosanoids in sevoflurane-mediated vasodilation. In separate experimental series, we examined whether the nonspecific cyclooxygenase inhibitor, indomethacin, or the 5-lipoxygenase inhibitor, nordihydroguaiaretic acid, modulated sevoflurane-mediated vasodilation.
Sevoflurane produced dose-dependent vasodilation of preconstricted chorionic plate arterial rings, with mean ring vasodilation increasing from 15 +/- 7% at 2% sevoflurane to 67 +/- 17% (mean +/- sd) at 8% sevoflurane. Blockade of NO synthase did not attenuate the vasodilator effects of sevoflurane. Sevoflurane did not alter smooth muscle sensitivity to NO. Indomethacin augmented sevoflurane vasodilation at 10(-5) M, but not at 10(-6) M. Conversely, nordihydroguaiaretic acid attenuated sevoflurane-mediated vasodilation at 3 x 10(-6) M but not at 3 x 10(-7) M.
Sevoflurane was a vasodilator in the feto-placental vasculature in this in vitro model. Sevoflurane-mediated vasodilation is NO and cyclooxygenase-independent and appears to be mediated in part via a lipoxygenase generated vasodilator eicosanoid.</description><subject>Anesthesia</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Anesthetics, Inhalation - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Dose-Response Relationship, Drug</subject><subject>Eicosanoids - physiology</subject><subject>Female</subject><subject>Fetus - blood supply</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Indomethacin - pharmacology</subject><subject>Masoprocol - pharmacology</subject><subject>Medical sciences</subject><subject>Methyl Ethers - pharmacology</subject><subject>Nitric Oxide - physiology</subject><subject>Nitroprusside - pharmacology</subject><subject>omega-N-Methylarginine - pharmacology</subject><subject>Placenta - blood supply</subject><subject>Pregnancy</subject><subject>Vasodilation - drug effects</subject><issn>0003-2999</issn><issn>1526-7598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkF9rFTEQxYMo9lr9BiJ5sW9b82d3k_gmpVWhtNJWX8NsMsuN5t7UJNvqtzflXiw0EMKQ3zkzcwh5y9kxF1x-gC0es4lxiZJrrmQ_DviMrPggxk4NRj8nK8aY7IQx5oC8KuVnKznT40tywPUoh14PK-Ku8S7NccnNjsLW07pGeoY1dd8iONxWiPQHFLdEqEvGj_Sm_V-liDTN9CLUHBy9_BP8TtzIBK6GO6SnwaUC2xR8eU1ezBALvtm_h-T72enNyZfu_PLz15NP553rxWg6DzhBDzPzSjFsw2quZ6-dk7LtOTHFnQYunfKjVsxLCRPoUUxKgJf9PMhDcrTzvc3p94Kl2k0oDmNsu6Wl2NFIoXujGtjvQJdTKRlne5vDBvJfy5l9CNc2hX0abpO92_sv0wb9o2ifZgPe74GWGMS5hepC-c8J1myU1o_971OsmMuvuNxjtmuEWNeWPZxBmk60CJhqRdeuMPIfbXeTeA</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Farragher, Rachel</creator><creator>Maharaj, Chrisen H.</creator><creator>Higgins, Brendan D.</creator><creator>Crowe, Sharon</creator><creator>Burke, Padvaic</creator><creator>Laffey, Christopher D.</creator><creator>Flynn, Noel M.</creator><creator>Laffey, John G.</creator><general>International Anesthesia Research Society</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080701</creationdate><title>Sevoflurane and the Feto-Placental Vasculature: The Role of Nitric Oxide and Vasoactive Eicosanoids</title><author>Farragher, Rachel ; Maharaj, Chrisen H. ; Higgins, Brendan D. ; Crowe, Sharon ; Burke, Padvaic ; Laffey, Christopher D. ; Flynn, Noel M. ; Laffey, John G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4269-daeba4af0d770e000818fd8cc330b0b071c8a13c7d6870d33aba862b72ad34f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Anesthesia</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Anesthetics, Inhalation - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Dose-Response Relationship, Drug</topic><topic>Eicosanoids - physiology</topic><topic>Female</topic><topic>Fetus - blood supply</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Indomethacin - pharmacology</topic><topic>Masoprocol - pharmacology</topic><topic>Medical sciences</topic><topic>Methyl Ethers - pharmacology</topic><topic>Nitric Oxide - physiology</topic><topic>Nitroprusside - pharmacology</topic><topic>omega-N-Methylarginine - pharmacology</topic><topic>Placenta - blood supply</topic><topic>Pregnancy</topic><topic>Vasodilation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Farragher, Rachel</creatorcontrib><creatorcontrib>Maharaj, Chrisen H.</creatorcontrib><creatorcontrib>Higgins, Brendan D.</creatorcontrib><creatorcontrib>Crowe, Sharon</creatorcontrib><creatorcontrib>Burke, Padvaic</creatorcontrib><creatorcontrib>Laffey, Christopher D.</creatorcontrib><creatorcontrib>Flynn, Noel M.</creatorcontrib><creatorcontrib>Laffey, John G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Anesthesia and analgesia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Farragher, Rachel</au><au>Maharaj, Chrisen H.</au><au>Higgins, Brendan D.</au><au>Crowe, Sharon</au><au>Burke, Padvaic</au><au>Laffey, Christopher D.</au><au>Flynn, Noel M.</au><au>Laffey, John G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sevoflurane and the Feto-Placental Vasculature: The Role of Nitric Oxide and Vasoactive Eicosanoids</atitle><jtitle>Anesthesia and analgesia</jtitle><addtitle>Anesth Analg</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>107</volume><issue>1</issue><spage>171</spage><epage>177</epage><pages>171-177</pages><issn>0003-2999</issn><eissn>1526-7598</eissn><coden>AACRAT</coden><abstract>The effects and mechanisms of action of volatile anesthetics on the feto-placental vasculature are not known. We aimed to quantify the vasoactive effects of sevoflurane and determine the role of nitric oxide (NO) and of vasoactive eicosanoids in mediating these effects in isolated human chorionic plate arterial rings.
Quadruplicate ex vivo human chorionic plate arterial rings were used in all studies. Series 1 quantified the vasodilation produced by sevoflurane in rings preconstricted with the thromboxane analog U46619. Series 2A-C examined the role of NO in sevoflurane-mediated vasodilation. In separate experiments, we examined the potential for the nonspecific NO inhibitors, L-NAME, L-nMMA, and the inactive D-NAME, to modulate the vasodilation produced by sevoflurane. Series 2D determined whether sevoflurane altered vascular smooth muscle sensitivity to exogenous NO. Series 3A-D examined the role of vasoactive eicosanoids in sevoflurane-mediated vasodilation. In separate experimental series, we examined whether the nonspecific cyclooxygenase inhibitor, indomethacin, or the 5-lipoxygenase inhibitor, nordihydroguaiaretic acid, modulated sevoflurane-mediated vasodilation.
Sevoflurane produced dose-dependent vasodilation of preconstricted chorionic plate arterial rings, with mean ring vasodilation increasing from 15 +/- 7% at 2% sevoflurane to 67 +/- 17% (mean +/- sd) at 8% sevoflurane. Blockade of NO synthase did not attenuate the vasodilator effects of sevoflurane. Sevoflurane did not alter smooth muscle sensitivity to NO. Indomethacin augmented sevoflurane vasodilation at 10(-5) M, but not at 10(-6) M. Conversely, nordihydroguaiaretic acid attenuated sevoflurane-mediated vasodilation at 3 x 10(-6) M but not at 3 x 10(-7) M.
Sevoflurane was a vasodilator in the feto-placental vasculature in this in vitro model. Sevoflurane-mediated vasodilation is NO and cyclooxygenase-independent and appears to be mediated in part via a lipoxygenase generated vasodilator eicosanoid.</abstract><cop>Hagerstown, MD</cop><pub>International Anesthesia Research Society</pub><pmid>18635485</pmid><doi>10.1213/ane.0b013e318173465e</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anesthesia Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Anesthetics, Inhalation - pharmacology Biological and medical sciences Dose-Response Relationship, Drug Eicosanoids - physiology Female Fetus - blood supply Humans In Vitro Techniques Indomethacin - pharmacology Masoprocol - pharmacology Medical sciences Methyl Ethers - pharmacology Nitric Oxide - physiology Nitroprusside - pharmacology omega-N-Methylarginine - pharmacology Placenta - blood supply Pregnancy Vasodilation - drug effects |
| title | Sevoflurane and the Feto-Placental Vasculature: The Role of Nitric Oxide and Vasoactive Eicosanoids |
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