Characterization of langerhans cells in epidermal sheets along the body of Armadillo ( Dasypus novemcinctus)
Armadillos are apparently important reservoirs of Mycobacterium leprae and an animal model for human leprosy, whose immune system has been poorly studied. We aimed at characterizing the armadillo's langerhans cells (LC) using epidermal sheets instead of tissue sections, since the latter restric...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2008-08, Vol.124 (3), p.220-229 |
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| creator | Quesada-Pascual, Fausto Jimenez-Flores, Rafael Flores-Langarica, Adriana Silva-Sanchez, Aaron Calderon-Amador, Juana Mendez-Cruz, Rene Limon-Flores, Alberto Y. Estrada-Parra, Sergio Santos-Argumedo, Leopoldo Estrada-Garcia, Iris Flores-Romo, Leopoldo |
| description | Armadillos are apparently important reservoirs of
Mycobacterium leprae and an animal model for human leprosy, whose immune system has been poorly studied. We aimed at characterizing the armadillo's langerhans cells (LC) using epidermal sheets instead of tissue sections, since the latter restrict analysis only to cut-traversed cells. Epidermal sheets by providing an
en face view, are particularly convenient to evaluate dendritic morphology (cells are complete), spatial distribution (regular vs. clustered), and frequency (cell number/tissue area). Lack of anti-armadillo antibodies was overcome using LC-restricted ATPase staining, allowing assessment of cell frequency, cell size, and dendrites extension. Average LC frequency in four animals was 528
LC/mm
2, showing a rather uniform non-clustered distribution, which increased towards the animal's head, while cell size increased towards the tail; without overt differences between sexes. The screening of antibodies to human DC (MHC-II, CD1a, langerin, CD86) in armadillo epidermal sheets, revealed positive cells with prominent dendritic morphology only with MHC-II and CD86. This allowed us to test DC mobilization from epidermis into dermis under topical oxazolone stimulation, a finding that was corroborated using whole skin conventional sections.
We hope that the characterization of armadillo's LC will incite studies of leprosy and immunity in this animal model. |
| doi_str_mv | 10.1016/j.vetimm.2008.03.011 |
| format | Article |
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Mycobacterium leprae and an animal model for human leprosy, whose immune system has been poorly studied. We aimed at characterizing the armadillo's langerhans cells (LC) using epidermal sheets instead of tissue sections, since the latter restrict analysis only to cut-traversed cells. Epidermal sheets by providing an
en face view, are particularly convenient to evaluate dendritic morphology (cells are complete), spatial distribution (regular vs. clustered), and frequency (cell number/tissue area). Lack of anti-armadillo antibodies was overcome using LC-restricted ATPase staining, allowing assessment of cell frequency, cell size, and dendrites extension. Average LC frequency in four animals was 528
LC/mm
2, showing a rather uniform non-clustered distribution, which increased towards the animal's head, while cell size increased towards the tail; without overt differences between sexes. The screening of antibodies to human DC (MHC-II, CD1a, langerin, CD86) in armadillo epidermal sheets, revealed positive cells with prominent dendritic morphology only with MHC-II and CD86. This allowed us to test DC mobilization from epidermis into dermis under topical oxazolone stimulation, a finding that was corroborated using whole skin conventional sections.
We hope that the characterization of armadillo's LC will incite studies of leprosy and immunity in this animal model.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2008.03.011</identifier><identifier>PMID: 18482772</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adenosine Triphosphatases - biosynthesis ; adenosinetriphosphatase ; Adjuvants, Immunologic - pharmacology ; ADP-ribosyl Cyclase 1 - immunology ; animal models ; Animals ; Antibodies - immunology ; antigen-presenting cells ; Armadillo ; Armadillos - anatomy & histology ; Armadillos - immunology ; biomarkers ; Biopsy - veterinary ; cell physiology ; Cross Reactions ; Dasypus ; Dasypus novemcinctus ; dendritic cells ; Dendritic Cells - drug effects ; Dendritic Cells - immunology ; epidermis (animal) ; Epidermis - cytology ; Epidermis - enzymology ; Epidermis - immunology ; Epithelial sheets ; Female ; HLA-DR Antigens - immunology ; human diseases ; immune response ; immune system ; Immunohistochemistry - veterinary ; In vivo ; Langerhans cells ; Langerhans Cells - cytology ; Langerhans Cells - enzymology ; Langerhans Cells - immunology ; Leprosy ; major histocompatibility complex ; Male ; mammals ; mycobacterial diseases ; Mycobacterium leprae ; Mycobacterium leprae reservoir ; Oxazolone - pharmacology ; pathogenesis ; skin</subject><ispartof>Veterinary immunology and immunopathology, 2008-08, Vol.124 (3), p.220-229</ispartof><rights>2008 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-b9e877316c6c89149ce4a2f48c091b36808ea5b539e3a6a4e3f4c6e7f39dda5e3</citedby><cites>FETCH-LOGICAL-c415t-b9e877316c6c89149ce4a2f48c091b36808ea5b539e3a6a4e3f4c6e7f39dda5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetimm.2008.03.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18482772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Quesada-Pascual, Fausto</creatorcontrib><creatorcontrib>Jimenez-Flores, Rafael</creatorcontrib><creatorcontrib>Flores-Langarica, Adriana</creatorcontrib><creatorcontrib>Silva-Sanchez, Aaron</creatorcontrib><creatorcontrib>Calderon-Amador, Juana</creatorcontrib><creatorcontrib>Mendez-Cruz, Rene</creatorcontrib><creatorcontrib>Limon-Flores, Alberto Y.</creatorcontrib><creatorcontrib>Estrada-Parra, Sergio</creatorcontrib><creatorcontrib>Santos-Argumedo, Leopoldo</creatorcontrib><creatorcontrib>Estrada-Garcia, Iris</creatorcontrib><creatorcontrib>Flores-Romo, Leopoldo</creatorcontrib><title>Characterization of langerhans cells in epidermal sheets along the body of Armadillo ( Dasypus novemcinctus)</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>Armadillos are apparently important reservoirs of
Mycobacterium leprae and an animal model for human leprosy, whose immune system has been poorly studied. We aimed at characterizing the armadillo's langerhans cells (LC) using epidermal sheets instead of tissue sections, since the latter restrict analysis only to cut-traversed cells. Epidermal sheets by providing an
en face view, are particularly convenient to evaluate dendritic morphology (cells are complete), spatial distribution (regular vs. clustered), and frequency (cell number/tissue area). Lack of anti-armadillo antibodies was overcome using LC-restricted ATPase staining, allowing assessment of cell frequency, cell size, and dendrites extension. Average LC frequency in four animals was 528
LC/mm
2, showing a rather uniform non-clustered distribution, which increased towards the animal's head, while cell size increased towards the tail; without overt differences between sexes. The screening of antibodies to human DC (MHC-II, CD1a, langerin, CD86) in armadillo epidermal sheets, revealed positive cells with prominent dendritic morphology only with MHC-II and CD86. This allowed us to test DC mobilization from epidermis into dermis under topical oxazolone stimulation, a finding that was corroborated using whole skin conventional sections.
We hope that the characterization of armadillo's LC will incite studies of leprosy and immunity in this animal model.</description><subject>Adenosine Triphosphatases - biosynthesis</subject><subject>adenosinetriphosphatase</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>ADP-ribosyl Cyclase 1 - immunology</subject><subject>animal models</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>antigen-presenting cells</subject><subject>Armadillo</subject><subject>Armadillos - anatomy & histology</subject><subject>Armadillos - immunology</subject><subject>biomarkers</subject><subject>Biopsy - veterinary</subject><subject>cell physiology</subject><subject>Cross Reactions</subject><subject>Dasypus</subject><subject>Dasypus novemcinctus</subject><subject>dendritic cells</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - immunology</subject><subject>epidermis (animal)</subject><subject>Epidermis - cytology</subject><subject>Epidermis - enzymology</subject><subject>Epidermis - immunology</subject><subject>Epithelial sheets</subject><subject>Female</subject><subject>HLA-DR Antigens - immunology</subject><subject>human diseases</subject><subject>immune response</subject><subject>immune system</subject><subject>Immunohistochemistry - veterinary</subject><subject>In vivo</subject><subject>Langerhans cells</subject><subject>Langerhans Cells - cytology</subject><subject>Langerhans Cells - enzymology</subject><subject>Langerhans Cells - immunology</subject><subject>Leprosy</subject><subject>major histocompatibility complex</subject><subject>Male</subject><subject>mammals</subject><subject>mycobacterial diseases</subject><subject>Mycobacterium leprae</subject><subject>Mycobacterium leprae reservoir</subject><subject>Oxazolone - pharmacology</subject><subject>pathogenesis</subject><subject>skin</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuv0zAQhS0E4pYL_wCBVwgWCeNHHHuDdFWe0pVYwF1bjjNpXSVxsZNK5deTKpXYwWoW852jmXMIecmgZMDU-0N5wikMQ8kBdAmiBMYekQ3TtSh4JeRjslmwquCS1zfkWc4HAKiM1k_JDdNS87rmG9Jv9y45P2EKv90U4khjR3s37jDt3Zipx77PNIwUj6HFNLie5j3ilKnr47ij0x5pE9vzRXa3rNvQ95G-pR9dPh_nTMd4wsGH0U9zfvecPOlcn_HFdd6Sh8-ffm6_Fvffv3zb3t0XXrJqKhqDuq4FU155bZg0HqXjndQeDGuE0qDRVU0lDAqnnETRSa-w7oRpW1ehuCVvVt9jir9mzJMdQr584kaMc7bKCK6YZv8FOWhlFMACyhX0KeacsLPHFAaXzpaBvdRhD3atw17qsCDsUscie3X1n5sB27-ia_4L8HoFOhet26WQ7cMPDkzA8motwSzEh5XAJbBTwGSzDzh6bENCP9k2hn_f8AcyE6f8</recordid><startdate>20080815</startdate><enddate>20080815</enddate><creator>Quesada-Pascual, Fausto</creator><creator>Jimenez-Flores, Rafael</creator><creator>Flores-Langarica, Adriana</creator><creator>Silva-Sanchez, Aaron</creator><creator>Calderon-Amador, Juana</creator><creator>Mendez-Cruz, Rene</creator><creator>Limon-Flores, Alberto Y.</creator><creator>Estrada-Parra, Sergio</creator><creator>Santos-Argumedo, Leopoldo</creator><creator>Estrada-Garcia, Iris</creator><creator>Flores-Romo, Leopoldo</creator><general>Elsevier B.V</general><general>Amsterdam: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080815</creationdate><title>Characterization of langerhans cells in epidermal sheets along the body of Armadillo ( Dasypus novemcinctus)</title><author>Quesada-Pascual, Fausto ; Jimenez-Flores, Rafael ; Flores-Langarica, Adriana ; Silva-Sanchez, Aaron ; Calderon-Amador, Juana ; Mendez-Cruz, Rene ; Limon-Flores, Alberto Y. ; Estrada-Parra, Sergio ; Santos-Argumedo, Leopoldo ; Estrada-Garcia, Iris ; Flores-Romo, Leopoldo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-b9e877316c6c89149ce4a2f48c091b36808ea5b539e3a6a4e3f4c6e7f39dda5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adenosine Triphosphatases - biosynthesis</topic><topic>adenosinetriphosphatase</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>ADP-ribosyl Cyclase 1 - immunology</topic><topic>animal models</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>antigen-presenting cells</topic><topic>Armadillo</topic><topic>Armadillos - anatomy & histology</topic><topic>Armadillos - immunology</topic><topic>biomarkers</topic><topic>Biopsy - veterinary</topic><topic>cell physiology</topic><topic>Cross Reactions</topic><topic>Dasypus</topic><topic>Dasypus novemcinctus</topic><topic>dendritic cells</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - immunology</topic><topic>epidermis (animal)</topic><topic>Epidermis - cytology</topic><topic>Epidermis - enzymology</topic><topic>Epidermis - immunology</topic><topic>Epithelial sheets</topic><topic>Female</topic><topic>HLA-DR Antigens - immunology</topic><topic>human diseases</topic><topic>immune response</topic><topic>immune system</topic><topic>Immunohistochemistry - veterinary</topic><topic>In vivo</topic><topic>Langerhans cells</topic><topic>Langerhans Cells - cytology</topic><topic>Langerhans Cells - enzymology</topic><topic>Langerhans Cells - immunology</topic><topic>Leprosy</topic><topic>major histocompatibility complex</topic><topic>Male</topic><topic>mammals</topic><topic>mycobacterial diseases</topic><topic>Mycobacterium leprae</topic><topic>Mycobacterium leprae reservoir</topic><topic>Oxazolone - pharmacology</topic><topic>pathogenesis</topic><topic>skin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Quesada-Pascual, Fausto</creatorcontrib><creatorcontrib>Jimenez-Flores, Rafael</creatorcontrib><creatorcontrib>Flores-Langarica, Adriana</creatorcontrib><creatorcontrib>Silva-Sanchez, Aaron</creatorcontrib><creatorcontrib>Calderon-Amador, Juana</creatorcontrib><creatorcontrib>Mendez-Cruz, Rene</creatorcontrib><creatorcontrib>Limon-Flores, Alberto Y.</creatorcontrib><creatorcontrib>Estrada-Parra, Sergio</creatorcontrib><creatorcontrib>Santos-Argumedo, Leopoldo</creatorcontrib><creatorcontrib>Estrada-Garcia, Iris</creatorcontrib><creatorcontrib>Flores-Romo, Leopoldo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Quesada-Pascual, Fausto</au><au>Jimenez-Flores, Rafael</au><au>Flores-Langarica, Adriana</au><au>Silva-Sanchez, Aaron</au><au>Calderon-Amador, Juana</au><au>Mendez-Cruz, Rene</au><au>Limon-Flores, Alberto Y.</au><au>Estrada-Parra, Sergio</au><au>Santos-Argumedo, Leopoldo</au><au>Estrada-Garcia, Iris</au><au>Flores-Romo, Leopoldo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of langerhans cells in epidermal sheets along the body of Armadillo ( Dasypus novemcinctus)</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2008-08-15</date><risdate>2008</risdate><volume>124</volume><issue>3</issue><spage>220</spage><epage>229</epage><pages>220-229</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>Armadillos are apparently important reservoirs of
Mycobacterium leprae and an animal model for human leprosy, whose immune system has been poorly studied. We aimed at characterizing the armadillo's langerhans cells (LC) using epidermal sheets instead of tissue sections, since the latter restrict analysis only to cut-traversed cells. Epidermal sheets by providing an
en face view, are particularly convenient to evaluate dendritic morphology (cells are complete), spatial distribution (regular vs. clustered), and frequency (cell number/tissue area). Lack of anti-armadillo antibodies was overcome using LC-restricted ATPase staining, allowing assessment of cell frequency, cell size, and dendrites extension. Average LC frequency in four animals was 528
LC/mm
2, showing a rather uniform non-clustered distribution, which increased towards the animal's head, while cell size increased towards the tail; without overt differences between sexes. The screening of antibodies to human DC (MHC-II, CD1a, langerin, CD86) in armadillo epidermal sheets, revealed positive cells with prominent dendritic morphology only with MHC-II and CD86. This allowed us to test DC mobilization from epidermis into dermis under topical oxazolone stimulation, a finding that was corroborated using whole skin conventional sections.
We hope that the characterization of armadillo's LC will incite studies of leprosy and immunity in this animal model.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18482772</pmid><doi>10.1016/j.vetimm.2008.03.011</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 2008-08, Vol.124 (3), p.220-229 |
| issn | 0165-2427 1873-2534 |
| language | eng |
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| subjects | Adenosine Triphosphatases - biosynthesis adenosinetriphosphatase Adjuvants, Immunologic - pharmacology ADP-ribosyl Cyclase 1 - immunology animal models Animals Antibodies - immunology antigen-presenting cells Armadillo Armadillos - anatomy & histology Armadillos - immunology biomarkers Biopsy - veterinary cell physiology Cross Reactions Dasypus Dasypus novemcinctus dendritic cells Dendritic Cells - drug effects Dendritic Cells - immunology epidermis (animal) Epidermis - cytology Epidermis - enzymology Epidermis - immunology Epithelial sheets Female HLA-DR Antigens - immunology human diseases immune response immune system Immunohistochemistry - veterinary In vivo Langerhans cells Langerhans Cells - cytology Langerhans Cells - enzymology Langerhans Cells - immunology Leprosy major histocompatibility complex Male mammals mycobacterial diseases Mycobacterium leprae Mycobacterium leprae reservoir Oxazolone - pharmacology pathogenesis skin |
| title | Characterization of langerhans cells in epidermal sheets along the body of Armadillo ( Dasypus novemcinctus) |
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