Immunogenicity in mice and rabbits of DNA vaccines expressing woodchuck hepatitis virus antigens
Abstract The licensed vaccine against hepatitis B virus (HBV) is an effective means to prevent infection, but is not an effective therapeutic strategy to treat established chronic infections when used alone. In an animal model of chronic HBV infection (the woodchuck experimentally infected with wood...
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| Veröffentlicht in: | Vaccine 2008-07, Vol.26 (32), p.4025-4033 |
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| creator | Luxembourg, Alain Hannaman, Drew Wills, Ken Bernard, Robert Tennant, Bud C Menne, Stephan Cote, Paul J |
| description | Abstract The licensed vaccine against hepatitis B virus (HBV) is an effective means to prevent infection, but is not an effective therapeutic strategy to treat established chronic infections when used alone. In an animal model of chronic HBV infection (the woodchuck experimentally infected with woodchuck hepatitis virus (WHV)), the combination of conventional vaccine and potent antiviral drugs has shown promise as a potential therapeutic intervention. This approach might be improved further through the application of newer vaccine technologies. In the present study, we evaluated electroporation (EP)-based intramuscular (i.m.) delivery of a codon-optimized DNA vaccine for the WHV surface antigen (WHsAg) in mice and rabbits. In mice, this immunization procedure compared favorably to vaccination by i.m. injection of the DNA vaccine or i.m. administration of a recombinant WHsAg–alum vaccine, exhibiting characteristics expected to be beneficial for a therapeutic vaccine strategy. These included dose efficiency, consistency, vigorous induction of antibody responses to WHsAg, as well as a Th1 bias. Following scale-up to rabbits, a species that approximates the size of the woodchuck, the EP dosing regimen was markedly more effective than conventional i.m. injection of the DNA vaccine. Taken together, these results provide the foundation for studies of EP-based DNA immunization in the woodchuck in order to further assess its potential as an immunotherapeutic approach for treatment of chronic HBV infection in humans. |
| doi_str_mv | 10.1016/j.vaccine.2008.05.021 |
| format | Article |
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In an animal model of chronic HBV infection (the woodchuck experimentally infected with woodchuck hepatitis virus (WHV)), the combination of conventional vaccine and potent antiviral drugs has shown promise as a potential therapeutic intervention. This approach might be improved further through the application of newer vaccine technologies. In the present study, we evaluated electroporation (EP)-based intramuscular (i.m.) delivery of a codon-optimized DNA vaccine for the WHV surface antigen (WHsAg) in mice and rabbits. In mice, this immunization procedure compared favorably to vaccination by i.m. injection of the DNA vaccine or i.m. administration of a recombinant WHsAg–alum vaccine, exhibiting characteristics expected to be beneficial for a therapeutic vaccine strategy. These included dose efficiency, consistency, vigorous induction of antibody responses to WHsAg, as well as a Th1 bias. Following scale-up to rabbits, a species that approximates the size of the woodchuck, the EP dosing regimen was markedly more effective than conventional i.m. injection of the DNA vaccine. Taken together, these results provide the foundation for studies of EP-based DNA immunization in the woodchuck in order to further assess its potential as an immunotherapeutic approach for treatment of chronic HBV infection in humans.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2008.05.021</identifier><identifier>PMID: 18556096</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Allergy and Immunology ; Animal models ; Animals ; Applied microbiology ; Biological and medical sciences ; Deoxyribonucleic acid ; Disease ; DNA ; DNA vaccine ; Drug resistance ; Electroporation ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - immunology ; Hepatitis ; Hepatitis B Antibodies - blood ; Hepatitis B Surface Antigens - chemistry ; Hepatitis B Surface Antigens - immunology ; Hepatitis B Vaccines - immunology ; Hepatitis B Vaccines - therapeutic use ; Hepatitis B virus ; Hepatitis B Virus, Woodchuck - immunology ; Immunization ; Immunogenicity ; Immunoglobulin G - blood ; Infections ; Injection ; Laboratory animals ; Lymphocytes ; Marmota - immunology ; Methods ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Rabbits ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - immunology ; Vaccines, DNA - therapeutic use ; Virology ; Woodchuck ; Woodchuck hepatitis virus</subject><ispartof>Vaccine, 2008-07, Vol.26 (32), p.4025-4033</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><rights>Copyright Elsevier Limited Jul 29, 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-93836ac02cb4bd278b59bac730f66d52403fa572a8bc8873c87c33e12723786f3</citedby><cites>FETCH-LOGICAL-c507t-93836ac02cb4bd278b59bac730f66d52403fa572a8bc8873c87c33e12723786f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1558848435?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,64361,64363,64365,65309,72215</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20557508$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18556096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luxembourg, Alain</creatorcontrib><creatorcontrib>Hannaman, Drew</creatorcontrib><creatorcontrib>Wills, Ken</creatorcontrib><creatorcontrib>Bernard, Robert</creatorcontrib><creatorcontrib>Tennant, Bud C</creatorcontrib><creatorcontrib>Menne, Stephan</creatorcontrib><creatorcontrib>Cote, Paul J</creatorcontrib><title>Immunogenicity in mice and rabbits of DNA vaccines expressing woodchuck hepatitis virus antigens</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract The licensed vaccine against hepatitis B virus (HBV) is an effective means to prevent infection, but is not an effective therapeutic strategy to treat established chronic infections when used alone. In an animal model of chronic HBV infection (the woodchuck experimentally infected with woodchuck hepatitis virus (WHV)), the combination of conventional vaccine and potent antiviral drugs has shown promise as a potential therapeutic intervention. This approach might be improved further through the application of newer vaccine technologies. In the present study, we evaluated electroporation (EP)-based intramuscular (i.m.) delivery of a codon-optimized DNA vaccine for the WHV surface antigen (WHsAg) in mice and rabbits. In mice, this immunization procedure compared favorably to vaccination by i.m. injection of the DNA vaccine or i.m. administration of a recombinant WHsAg–alum vaccine, exhibiting characteristics expected to be beneficial for a therapeutic vaccine strategy. These included dose efficiency, consistency, vigorous induction of antibody responses to WHsAg, as well as a Th1 bias. Following scale-up to rabbits, a species that approximates the size of the woodchuck, the EP dosing regimen was markedly more effective than conventional i.m. injection of the DNA vaccine. Taken together, these results provide the foundation for studies of EP-based DNA immunization in the woodchuck in order to further assess its potential as an immunotherapeutic approach for treatment of chronic HBV infection in humans.</description><subject>Allergy and Immunology</subject><subject>Animal models</subject><subject>Animals</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>DNA</subject><subject>DNA vaccine</subject><subject>Drug resistance</subject><subject>Electroporation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - immunology</subject><subject>Hepatitis</subject><subject>Hepatitis B Antibodies - blood</subject><subject>Hepatitis B Surface Antigens - chemistry</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>Hepatitis B Vaccines - immunology</subject><subject>Hepatitis B Vaccines - therapeutic use</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B Virus, Woodchuck - immunology</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunoglobulin G - blood</subject><subject>Infections</subject><subject>Injection</subject><subject>Laboratory animals</subject><subject>Lymphocytes</subject><subject>Marmota - immunology</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Rabbits</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, DNA - immunology</subject><subject>Vaccines, DNA - therapeutic use</subject><subject>Virology</subject><subject>Woodchuck</subject><subject>Woodchuck hepatitis virus</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkk9v1DAQxSMEokvhI4AsIbgljO3YcS6gqvyrVMEBkLgZZ-K03ibOYicL--1xtBGVeunJl988z5v3suw5hYIClW-2xd4gOm8LBqAKEAUw-iDbUFXxnAmqHmYbYLLMSwo_T7InMW4BQHBaP85OqBJCQi032a-LYZj9eGW9QzcdiPNkcGiJ8S0JpmncFMnYkfdfzsj6XyT27y7YGJ2_In_GscXrGW_Itd2ZyU0ukr0Lc0wCk0uq8Wn2qDN9tM_W9zT78fHD9_PP-eXXTxfnZ5c5CqimvOaKS4PAsCmbllWqEXVjsOLQSdkKVgLvjKiYUQ2qZBFVhZxbyirGKyU7fpq9Puruwvh7tnHSg4to-954O85Ry5qzZJndC9Ja1unCKoEv74DbcQ4-mdBUCKVKVXKRKHGkMIwxBtvpXXCDCQdNQS9J6a1eL6eXpDQInZJKcy9W9bkZbHs7tUaTgFcrYCKavgvGo4v_OQZCVAKWNd8dOZuuu3c26IjOerStCxYn3Y7u3lXe3lHA3qU-mP7GHmy8da0j06C_LbVaWgUqFaquBP8HgaTIYQ</recordid><startdate>20080729</startdate><enddate>20080729</enddate><creator>Luxembourg, Alain</creator><creator>Hannaman, Drew</creator><creator>Wills, Ken</creator><creator>Bernard, Robert</creator><creator>Tennant, Bud C</creator><creator>Menne, Stephan</creator><creator>Cote, Paul J</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20080729</creationdate><title>Immunogenicity in mice and rabbits of DNA vaccines expressing woodchuck hepatitis virus antigens</title><author>Luxembourg, Alain ; Hannaman, Drew ; Wills, Ken ; Bernard, Robert ; Tennant, Bud C ; Menne, Stephan ; Cote, Paul J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-93836ac02cb4bd278b59bac730f66d52403fa572a8bc8873c87c33e12723786f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Allergy and Immunology</topic><topic>Animal models</topic><topic>Animals</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleic acid</topic><topic>Disease</topic><topic>DNA</topic><topic>DNA vaccine</topic><topic>Drug resistance</topic><topic>Electroporation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luxembourg, Alain</au><au>Hannaman, Drew</au><au>Wills, Ken</au><au>Bernard, Robert</au><au>Tennant, Bud C</au><au>Menne, Stephan</au><au>Cote, Paul J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunogenicity in mice and rabbits of DNA vaccines expressing woodchuck hepatitis virus antigens</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2008-07-29</date><risdate>2008</risdate><volume>26</volume><issue>32</issue><spage>4025</spage><epage>4033</epage><pages>4025-4033</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract The licensed vaccine against hepatitis B virus (HBV) is an effective means to prevent infection, but is not an effective therapeutic strategy to treat established chronic infections when used alone. In an animal model of chronic HBV infection (the woodchuck experimentally infected with woodchuck hepatitis virus (WHV)), the combination of conventional vaccine and potent antiviral drugs has shown promise as a potential therapeutic intervention. This approach might be improved further through the application of newer vaccine technologies. In the present study, we evaluated electroporation (EP)-based intramuscular (i.m.) delivery of a codon-optimized DNA vaccine for the WHV surface antigen (WHsAg) in mice and rabbits. In mice, this immunization procedure compared favorably to vaccination by i.m. injection of the DNA vaccine or i.m. administration of a recombinant WHsAg–alum vaccine, exhibiting characteristics expected to be beneficial for a therapeutic vaccine strategy. These included dose efficiency, consistency, vigorous induction of antibody responses to WHsAg, as well as a Th1 bias. Following scale-up to rabbits, a species that approximates the size of the woodchuck, the EP dosing regimen was markedly more effective than conventional i.m. injection of the DNA vaccine. Taken together, these results provide the foundation for studies of EP-based DNA immunization in the woodchuck in order to further assess its potential as an immunotherapeutic approach for treatment of chronic HBV infection in humans.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18556096</pmid><doi>10.1016/j.vaccine.2008.05.021</doi><tpages>9</tpages></addata></record> |
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| ispartof | Vaccine, 2008-07, Vol.26 (32), p.4025-4033 |
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| subjects | Allergy and Immunology Animal models Animals Applied microbiology Biological and medical sciences Deoxyribonucleic acid Disease DNA DNA vaccine Drug resistance Electroporation Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation - immunology Hepatitis Hepatitis B Antibodies - blood Hepatitis B Surface Antigens - chemistry Hepatitis B Surface Antigens - immunology Hepatitis B Vaccines - immunology Hepatitis B Vaccines - therapeutic use Hepatitis B virus Hepatitis B Virus, Woodchuck - immunology Immunization Immunogenicity Immunoglobulin G - blood Infections Injection Laboratory animals Lymphocytes Marmota - immunology Methods Mice Mice, Inbred BALB C Microbiology Miscellaneous Rabbits Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - immunology Vaccines, DNA - therapeutic use Virology Woodchuck Woodchuck hepatitis virus |
| title | Immunogenicity in mice and rabbits of DNA vaccines expressing woodchuck hepatitis virus antigens |
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