Transcriptome analysis of auxin-regulated genes of Arabidopsis thaliana
Plant hormone auxin elicits diverse responses in plant growth and development. Accumulated data indicate that the ubiquitin-mediated proteolytic pathway plays a crucial role in transducing auxin signaling. To gain more understanding of the molecular mechanisms underlying auxin action, we performed a...
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| Veröffentlicht in: | Gene 2008-09, Vol.420 (2), p.118-124 |
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| creator | Huang, Yung-Chieh Chang, Yueh-Long Hsu, Jen-Jen Chuang, Huey-wen |
| description | Plant hormone auxin elicits diverse responses in plant growth and development. Accumulated data indicate that the ubiquitin-mediated proteolytic pathway plays a crucial role in transducing auxin signaling. To gain more understanding of the molecular mechanisms underlying auxin action, we performed a comparative transcriptome analysis of auxin responsive genes between
Arabidopsis Columbia ecotype and the auxin insensitive mutant
eta2 by cDNA-AFLP. Using 256 primer combinations, about 5900 transcript-derived fragments (TDFs) were amplified. Sixty-six differentially expressed TDFs were confirmed by DNA dot blot analysis. Sequence analysis indicated that, a large number of genes involved in transcription regulation or RNA metabolism were identified as auxin-regulated genes. Northern blot analyses confirmed transcription levels of 16 auxin-regulated genes. These genes include various forms of transcription regulators, defense related, RING-type ubiquitin ligases, and glycosyl hydrolase. This study demonstrates that auxin exerts its effect in complex transcriptional networks. |
| doi_str_mv | 10.1016/j.gene.2008.05.017 |
| format | Article |
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Arabidopsis Columbia ecotype and the auxin insensitive mutant
eta2 by cDNA-AFLP. Using 256 primer combinations, about 5900 transcript-derived fragments (TDFs) were amplified. Sixty-six differentially expressed TDFs were confirmed by DNA dot blot analysis. Sequence analysis indicated that, a large number of genes involved in transcription regulation or RNA metabolism were identified as auxin-regulated genes. Northern blot analyses confirmed transcription levels of 16 auxin-regulated genes. These genes include various forms of transcription regulators, defense related, RING-type ubiquitin ligases, and glycosyl hydrolase. This study demonstrates that auxin exerts its effect in complex transcriptional networks.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2008.05.017</identifier><identifier>PMID: 18577427</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; cDNA-AFLP ; DNA, Complementary - metabolism ; ETA2 ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genes, Plant ; Indoleacetic Acids - pharmacology ; Molecular Sequence Data ; Plant Growth Regulators - pharmacology ; Sequence Alignment ; Transcription factors ; Transcription profiling ; Transcription, Genetic - drug effects</subject><ispartof>Gene, 2008-09, Vol.420 (2), p.118-124</ispartof><rights>2008 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-aec209f674827d0957bfbf59f51df3d317275d7417a696819da13325970fca393</citedby><cites>FETCH-LOGICAL-c385t-aec209f674827d0957bfbf59f51df3d317275d7417a696819da13325970fca393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378111908002254$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18577427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yung-Chieh</creatorcontrib><creatorcontrib>Chang, Yueh-Long</creatorcontrib><creatorcontrib>Hsu, Jen-Jen</creatorcontrib><creatorcontrib>Chuang, Huey-wen</creatorcontrib><title>Transcriptome analysis of auxin-regulated genes of Arabidopsis thaliana</title><title>Gene</title><addtitle>Gene</addtitle><description>Plant hormone auxin elicits diverse responses in plant growth and development. Accumulated data indicate that the ubiquitin-mediated proteolytic pathway plays a crucial role in transducing auxin signaling. To gain more understanding of the molecular mechanisms underlying auxin action, we performed a comparative transcriptome analysis of auxin responsive genes between
Arabidopsis Columbia ecotype and the auxin insensitive mutant
eta2 by cDNA-AFLP. Using 256 primer combinations, about 5900 transcript-derived fragments (TDFs) were amplified. Sixty-six differentially expressed TDFs were confirmed by DNA dot blot analysis. Sequence analysis indicated that, a large number of genes involved in transcription regulation or RNA metabolism were identified as auxin-regulated genes. Northern blot analyses confirmed transcription levels of 16 auxin-regulated genes. These genes include various forms of transcription regulators, defense related, RING-type ubiquitin ligases, and glycosyl hydrolase. 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Accumulated data indicate that the ubiquitin-mediated proteolytic pathway plays a crucial role in transducing auxin signaling. To gain more understanding of the molecular mechanisms underlying auxin action, we performed a comparative transcriptome analysis of auxin responsive genes between
Arabidopsis Columbia ecotype and the auxin insensitive mutant
eta2 by cDNA-AFLP. Using 256 primer combinations, about 5900 transcript-derived fragments (TDFs) were amplified. Sixty-six differentially expressed TDFs were confirmed by DNA dot blot analysis. Sequence analysis indicated that, a large number of genes involved in transcription regulation or RNA metabolism were identified as auxin-regulated genes. Northern blot analyses confirmed transcription levels of 16 auxin-regulated genes. These genes include various forms of transcription regulators, defense related, RING-type ubiquitin ligases, and glycosyl hydrolase. This study demonstrates that auxin exerts its effect in complex transcriptional networks.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18577427</pmid><doi>10.1016/j.gene.2008.05.017</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana cDNA-AFLP DNA, Complementary - metabolism ETA2 Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Indoleacetic Acids - pharmacology Molecular Sequence Data Plant Growth Regulators - pharmacology Sequence Alignment Transcription factors Transcription profiling Transcription, Genetic - drug effects |
| title | Transcriptome analysis of auxin-regulated genes of Arabidopsis thaliana |
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