Analysis of steroids in yeast-mediated cell culture by on-line solid-phase extraction coupled high-performance liquid chromatography electrospray-ionization/mass spectrometry and novel continuous postcolumn infusion of internal standard technique

The reduction of 17-ketosteroid estrone or androstenedione to corresponding 17α- and 17β-estradiol or testosterone and epitestosterone has been performed with Saccharomyces cerevisiae. In the analysis of the cell culture, the solid-phase extraction (SPE) method was on-line coupled to high-performanc...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytica chimica acta 2008-08, Vol.623 (2), p.168-177
Hauptverfasser:	Cheng, Cheanyeh, Tsai, Hsiang-Rong
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Androgens Androstenedione - analysis Androstenedione - metabolism Cells, Cultured Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Estrogens Estrone - analysis Estrone - metabolism Exact sciences and technology Liquid chromatography electrospray-ionization/mass spectrometry Other chromatographic methods Oxidation-Reduction Postcolumn infusion of internal standard Reference Standards Saccharomyces cerevisiae Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - metabolism Sensitivity and Specificity Solid Phase Extraction - methods Solid-phase extraction Spectrometric and optical methods Spectrometry, Mass, Electrospray Ionization - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!