Immunohistochemical Analysis of Nestin, Osteopontin, and Proliferating Cells in the Reparative Process of Exposed Dental Pulp Capped with Mineral Trioxide Aggregate
Abstract This study investigated the reparative process of mechanically exposed pulps capped with mineral trioxide aggregate (MTA). Maxillary first molars of 8-week-old rats were MTA-capped for 1–14 days, and 5-bromo-2′-deoxyuridine–labeled proliferating cells and immunoreactivity for nestin and ost...
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| Veröffentlicht in: | Journal of endodontics 2008-08, Vol.34 (8), p.970-974 |
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| creator | Kuratate, Momoko, DDS Yoshiba, Kunihiko, DDS, PhD Shigetani, Yoshimi, DDS, PhD Yoshiba, Nagako, DDS, PhD Ohshima, Hayato, DDS, PhD Okiji, Takashi, DDS, PhD |
| description | Abstract This study investigated the reparative process of mechanically exposed pulps capped with mineral trioxide aggregate (MTA). Maxillary first molars of 8-week-old rats were MTA-capped for 1–14 days, and 5-bromo-2′-deoxyuridine–labeled proliferating cells and immunoreactivity for nestin and osteopontin were analyzed. MTA capping caused mild necrotic changes followed by progressive new matrix formation and calcified bridging. Proliferating cells peaked at 3 days when matrix formation was inconspicuous. Nestin-expressing cells appeared at 3 days, were arranged beneath the newly formed matrix at 5 days, and showed odontoblast-like morphology by 14 days. Osteopontin immunoreactivity was detected just beneath the necrotic area after 1 day. These findings suggest that pulpal responses to MTA capping involve proliferation and migration of progenitors followed by their differentiation into odontoblast-like cells, a mechanism basically similar to those to calcium hydroxide. Osteopontin might play a triggering role in initiation of the pulpal reparative process. |
| doi_str_mv | 10.1016/j.joen.2008.03.021 |
| format | Article |
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Maxillary first molars of 8-week-old rats were MTA-capped for 1–14 days, and 5-bromo-2′-deoxyuridine–labeled proliferating cells and immunoreactivity for nestin and osteopontin were analyzed. MTA capping caused mild necrotic changes followed by progressive new matrix formation and calcified bridging. Proliferating cells peaked at 3 days when matrix formation was inconspicuous. Nestin-expressing cells appeared at 3 days, were arranged beneath the newly formed matrix at 5 days, and showed odontoblast-like morphology by 14 days. Osteopontin immunoreactivity was detected just beneath the necrotic area after 1 day. These findings suggest that pulpal responses to MTA capping involve proliferation and migration of progenitors followed by their differentiation into odontoblast-like cells, a mechanism basically similar to those to calcium hydroxide. Osteopontin might play a triggering role in initiation of the pulpal reparative process.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2008.03.021</identifier><identifier>PMID: 18634929</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aluminum Compounds - pharmacology ; Animals ; Calcium Compounds - pharmacology ; Cell Proliferation ; Dental Pulp - drug effects ; Dental Pulp - metabolism ; Dental Pulp - physiology ; Dental Pulp - secretion ; Dental Pulp Capping - methods ; Dental Pulp Exposure - therapy ; Dentin, Secondary - growth & development ; Dentin, Secondary - secretion ; Dentistry ; Drug Combinations ; Endocrinology & Metabolism ; Immunoenzyme Techniques ; Intermediate Filament Proteins - biosynthesis ; Male ; Mineral trioxide aggregate ; Nerve Tissue Proteins - biosynthesis ; Nestin ; Odontoblasts - cytology ; osteopontin ; Osteopontin - biosynthesis ; Oxides - pharmacology ; pulp capping ; Rats ; Rats, Wistar ; Regeneration - physiology ; reparative dentinogenesis ; Silicates - pharmacology</subject><ispartof>Journal of endodontics, 2008-08, Vol.34 (8), p.970-974</ispartof><rights>American Association of Endodontists</rights><rights>2008 American Association of Endodontists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-b1ab48829de1307f00afd9ae3b64dfbfe96acae9f8711d1bef436eb864d9e9a33</citedby><cites>FETCH-LOGICAL-c409t-b1ab48829de1307f00afd9ae3b64dfbfe96acae9f8711d1bef436eb864d9e9a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S009923990800277X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18634929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuratate, Momoko, DDS</creatorcontrib><creatorcontrib>Yoshiba, Kunihiko, DDS, PhD</creatorcontrib><creatorcontrib>Shigetani, Yoshimi, DDS, PhD</creatorcontrib><creatorcontrib>Yoshiba, Nagako, DDS, PhD</creatorcontrib><creatorcontrib>Ohshima, Hayato, DDS, PhD</creatorcontrib><creatorcontrib>Okiji, Takashi, DDS, PhD</creatorcontrib><title>Immunohistochemical Analysis of Nestin, Osteopontin, and Proliferating Cells in the Reparative Process of Exposed Dental Pulp Capped with Mineral Trioxide Aggregate</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract This study investigated the reparative process of mechanically exposed pulps capped with mineral trioxide aggregate (MTA). Maxillary first molars of 8-week-old rats were MTA-capped for 1–14 days, and 5-bromo-2′-deoxyuridine–labeled proliferating cells and immunoreactivity for nestin and osteopontin were analyzed. MTA capping caused mild necrotic changes followed by progressive new matrix formation and calcified bridging. Proliferating cells peaked at 3 days when matrix formation was inconspicuous. Nestin-expressing cells appeared at 3 days, were arranged beneath the newly formed matrix at 5 days, and showed odontoblast-like morphology by 14 days. Osteopontin immunoreactivity was detected just beneath the necrotic area after 1 day. These findings suggest that pulpal responses to MTA capping involve proliferation and migration of progenitors followed by their differentiation into odontoblast-like cells, a mechanism basically similar to those to calcium hydroxide. Osteopontin might play a triggering role in initiation of the pulpal reparative process.</description><subject>Aluminum Compounds - pharmacology</subject><subject>Animals</subject><subject>Calcium Compounds - pharmacology</subject><subject>Cell Proliferation</subject><subject>Dental Pulp - drug effects</subject><subject>Dental Pulp - metabolism</subject><subject>Dental Pulp - physiology</subject><subject>Dental Pulp - secretion</subject><subject>Dental Pulp Capping - methods</subject><subject>Dental Pulp Exposure - therapy</subject><subject>Dentin, Secondary - growth & development</subject><subject>Dentin, Secondary - secretion</subject><subject>Dentistry</subject><subject>Drug Combinations</subject><subject>Endocrinology & Metabolism</subject><subject>Immunoenzyme Techniques</subject><subject>Intermediate Filament Proteins - biosynthesis</subject><subject>Male</subject><subject>Mineral trioxide aggregate</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nestin</subject><subject>Odontoblasts - cytology</subject><subject>osteopontin</subject><subject>Osteopontin - biosynthesis</subject><subject>Oxides - pharmacology</subject><subject>pulp capping</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Regeneration - physiology</subject><subject>reparative dentinogenesis</subject><subject>Silicates - pharmacology</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ksFu1DAURS0EokPhB1ggr1iRYMeZJJYQ0mhooVKhFRSJneXYzzMeEjvYSdv5Hz4UpzMSEgtWlp_vvdbVeQi9pCSnhFZvd_nOg8sLQpqcsJwU9BFa0KZuMrZclo_RghDOs4JxfoKexbgjhNaM1U_RCW0qVvKCL9Dvi76fnN_aOHq1hd4q2eGVk90-2oi9wV8gjta9wVdxBD9493CRTuPr4DtrIMg02eA1dF3E1uFxC_grDHKe38KsUhAfks7uBx9B4w_gxvTJ9dQNeC2HIY3u7LjFn61LaR2-CdbfWw14tdkE2MgRnqMnRnYRXhzPU_T9_Oxm_Sm7vPp4sV5dZqokfMxaKtuyaQqugTJSG0Kk0VwCa6tSm9YAr6SSwE1TU6ppC6ZkFbRNeuXAJWOn6PUhdwj-15SKi95GlZpJB36KouKMcr6kSVgchCr4GAMYMQTby7AXlIiZjdiJmY2Y2QjCRGKTTK-O6VPbg_5rOcJIgncHAaSOtxaCiMqCU6BtADUK7e3_89__Y1eddTPQn7CHuPNTSFyjoCIWgohv83bMy0EaQoq6_sH-AE07uaE</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Kuratate, Momoko, DDS</creator><creator>Yoshiba, Kunihiko, DDS, PhD</creator><creator>Shigetani, Yoshimi, DDS, PhD</creator><creator>Yoshiba, Nagako, DDS, PhD</creator><creator>Ohshima, Hayato, DDS, PhD</creator><creator>Okiji, Takashi, DDS, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080801</creationdate><title>Immunohistochemical Analysis of Nestin, Osteopontin, and Proliferating Cells in the Reparative Process of Exposed Dental Pulp Capped with Mineral Trioxide Aggregate</title><author>Kuratate, Momoko, DDS ; Yoshiba, Kunihiko, DDS, PhD ; Shigetani, Yoshimi, DDS, PhD ; Yoshiba, Nagako, DDS, PhD ; Ohshima, Hayato, DDS, PhD ; Okiji, Takashi, DDS, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-b1ab48829de1307f00afd9ae3b64dfbfe96acae9f8711d1bef436eb864d9e9a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aluminum Compounds - pharmacology</topic><topic>Animals</topic><topic>Calcium Compounds - pharmacology</topic><topic>Cell Proliferation</topic><topic>Dental Pulp - drug effects</topic><topic>Dental Pulp - metabolism</topic><topic>Dental Pulp - physiology</topic><topic>Dental Pulp - secretion</topic><topic>Dental Pulp Capping - methods</topic><topic>Dental Pulp Exposure - therapy</topic><topic>Dentin, Secondary - growth & development</topic><topic>Dentin, Secondary - secretion</topic><topic>Dentistry</topic><topic>Drug Combinations</topic><topic>Endocrinology & Metabolism</topic><topic>Immunoenzyme Techniques</topic><topic>Intermediate Filament Proteins - biosynthesis</topic><topic>Male</topic><topic>Mineral trioxide aggregate</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nestin</topic><topic>Odontoblasts - cytology</topic><topic>osteopontin</topic><topic>Osteopontin - biosynthesis</topic><topic>Oxides - pharmacology</topic><topic>pulp capping</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Regeneration - physiology</topic><topic>reparative dentinogenesis</topic><topic>Silicates - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuratate, Momoko, DDS</creatorcontrib><creatorcontrib>Yoshiba, Kunihiko, DDS, PhD</creatorcontrib><creatorcontrib>Shigetani, Yoshimi, DDS, PhD</creatorcontrib><creatorcontrib>Yoshiba, Nagako, DDS, PhD</creatorcontrib><creatorcontrib>Ohshima, Hayato, DDS, PhD</creatorcontrib><creatorcontrib>Okiji, Takashi, DDS, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuratate, Momoko, DDS</au><au>Yoshiba, Kunihiko, DDS, PhD</au><au>Shigetani, Yoshimi, DDS, PhD</au><au>Yoshiba, Nagako, DDS, PhD</au><au>Ohshima, Hayato, DDS, PhD</au><au>Okiji, Takashi, DDS, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical Analysis of Nestin, Osteopontin, and Proliferating Cells in the Reparative Process of Exposed Dental Pulp Capped with Mineral Trioxide Aggregate</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>34</volume><issue>8</issue><spage>970</spage><epage>974</epage><pages>970-974</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract This study investigated the reparative process of mechanically exposed pulps capped with mineral trioxide aggregate (MTA). Maxillary first molars of 8-week-old rats were MTA-capped for 1–14 days, and 5-bromo-2′-deoxyuridine–labeled proliferating cells and immunoreactivity for nestin and osteopontin were analyzed. MTA capping caused mild necrotic changes followed by progressive new matrix formation and calcified bridging. Proliferating cells peaked at 3 days when matrix formation was inconspicuous. Nestin-expressing cells appeared at 3 days, were arranged beneath the newly formed matrix at 5 days, and showed odontoblast-like morphology by 14 days. Osteopontin immunoreactivity was detected just beneath the necrotic area after 1 day. These findings suggest that pulpal responses to MTA capping involve proliferation and migration of progenitors followed by their differentiation into odontoblast-like cells, a mechanism basically similar to those to calcium hydroxide. Osteopontin might play a triggering role in initiation of the pulpal reparative process.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18634929</pmid><doi>10.1016/j.joen.2008.03.021</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of endodontics, 2008-08, Vol.34 (8), p.970-974 |
| issn | 0099-2399 1878-3554 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aluminum Compounds - pharmacology Animals Calcium Compounds - pharmacology Cell Proliferation Dental Pulp - drug effects Dental Pulp - metabolism Dental Pulp - physiology Dental Pulp - secretion Dental Pulp Capping - methods Dental Pulp Exposure - therapy Dentin, Secondary - growth & development Dentin, Secondary - secretion Dentistry Drug Combinations Endocrinology & Metabolism Immunoenzyme Techniques Intermediate Filament Proteins - biosynthesis Male Mineral trioxide aggregate Nerve Tissue Proteins - biosynthesis Nestin Odontoblasts - cytology osteopontin Osteopontin - biosynthesis Oxides - pharmacology pulp capping Rats Rats, Wistar Regeneration - physiology reparative dentinogenesis Silicates - pharmacology |
| title | Immunohistochemical Analysis of Nestin, Osteopontin, and Proliferating Cells in the Reparative Process of Exposed Dental Pulp Capped with Mineral Trioxide Aggregate |
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