Aluminium induces changes in organic acids metabolism in Coffea arabica suspension cells with differential Al-tolerance
The primary Al-tolerance mechanism in plants involves exudation and/or accumulation of specific organic acid species, which form non-phytotoxic complexes with Al 3+ under physiological conditions. An evaluation was done of the role of organic acids in the tolerance mechanism of a cell suspension lin...
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| Veröffentlicht in: | Journal of inorganic biochemistry 2008-08, Vol.102 (8), p.1631-1637 |
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| container_title | Journal of inorganic biochemistry |
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| creator | Efraín Ramírez-Benítez, J. Chee-González, Leticia Teresa Hernandez-Sotomayor, S.M. |
| description | The primary Al-tolerance mechanism in plants involves exudation and/or accumulation of specific organic acid species, which form non-phytotoxic complexes with Al
3+ under physiological conditions. An evaluation was done of the role of organic acids in the tolerance mechanism of a cell suspension line of coffee
Coffea arabica that exhibits Al-tolerance (LAMt) but for which the metabolic tolerance mechanism remains unknown. Significant differences existed in malate dehydrogenase and citrate synthase activities (key enzymes in organic acids metabolism) between protein extracts (day 7 of culture cycle) of the L2 (Al-sensitive) and LAMt (Al-tolerant) cells when cell suspensions were treated with 100
μM AlCl
3. HPLC analysis showed that the suspension cells of both lines exudate malate when incubated in a minimal solution but that exudation was not enhanced by treatment with AlCl
3 (100
μM). This is the first study demonstrating that plant Al-tolerance may be associated with down-regulation of malate dehydrogenase and citrate synthase activities. |
| doi_str_mv | 10.1016/j.jinorgbio.2008.03.002 |
| format | Article |
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3+ under physiological conditions. An evaluation was done of the role of organic acids in the tolerance mechanism of a cell suspension line of coffee
Coffea arabica that exhibits Al-tolerance (LAMt) but for which the metabolic tolerance mechanism remains unknown. Significant differences existed in malate dehydrogenase and citrate synthase activities (key enzymes in organic acids metabolism) between protein extracts (day 7 of culture cycle) of the L2 (Al-sensitive) and LAMt (Al-tolerant) cells when cell suspensions were treated with 100
μM AlCl
3. HPLC analysis showed that the suspension cells of both lines exudate malate when incubated in a minimal solution but that exudation was not enhanced by treatment with AlCl
3 (100
μM). This is the first study demonstrating that plant Al-tolerance may be associated with down-regulation of malate dehydrogenase and citrate synthase activities.</description><identifier>ISSN: 0162-0134</identifier><identifier>EISSN: 1873-3344</identifier><identifier>DOI: 10.1016/j.jinorgbio.2008.03.002</identifier><identifier>PMID: 18482769</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aluminium tolerance ; Aluminum - pharmacology ; Carboxylic Acids - metabolism ; Citrate (si)-Synthase - drug effects ; Citrate synthase ; Coffea - cytology ; Coffea - drug effects ; Coffea - enzymology ; Coffea - metabolism ; Coffea arabica ; Down-Regulation - drug effects ; Drug Tolerance ; Malate dehydrogenase ; Malate Dehydrogenase - drug effects ; Malates</subject><ispartof>Journal of inorganic biochemistry, 2008-08, Vol.102 (8), p.1631-1637</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-22a3e96ea110de6f3af8c63d429bfabad67cb43914e6cc9ce413d79f0ad0de883</citedby><cites>FETCH-LOGICAL-c369t-22a3e96ea110de6f3af8c63d429bfabad67cb43914e6cc9ce413d79f0ad0de883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jinorgbio.2008.03.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18482769$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Efraín Ramírez-Benítez, J.</creatorcontrib><creatorcontrib>Chee-González, Leticia</creatorcontrib><creatorcontrib>Teresa Hernandez-Sotomayor, S.M.</creatorcontrib><title>Aluminium induces changes in organic acids metabolism in Coffea arabica suspension cells with differential Al-tolerance</title><title>Journal of inorganic biochemistry</title><addtitle>J Inorg Biochem</addtitle><description>The primary Al-tolerance mechanism in plants involves exudation and/or accumulation of specific organic acid species, which form non-phytotoxic complexes with Al
3+ under physiological conditions. An evaluation was done of the role of organic acids in the tolerance mechanism of a cell suspension line of coffee
Coffea arabica that exhibits Al-tolerance (LAMt) but for which the metabolic tolerance mechanism remains unknown. Significant differences existed in malate dehydrogenase and citrate synthase activities (key enzymes in organic acids metabolism) between protein extracts (day 7 of culture cycle) of the L2 (Al-sensitive) and LAMt (Al-tolerant) cells when cell suspensions were treated with 100
μM AlCl
3. HPLC analysis showed that the suspension cells of both lines exudate malate when incubated in a minimal solution but that exudation was not enhanced by treatment with AlCl
3 (100
μM). This is the first study demonstrating that plant Al-tolerance may be associated with down-regulation of malate dehydrogenase and citrate synthase activities.</description><subject>Aluminium tolerance</subject><subject>Aluminum - pharmacology</subject><subject>Carboxylic Acids - metabolism</subject><subject>Citrate (si)-Synthase - drug effects</subject><subject>Citrate synthase</subject><subject>Coffea - cytology</subject><subject>Coffea - drug effects</subject><subject>Coffea - enzymology</subject><subject>Coffea - metabolism</subject><subject>Coffea arabica</subject><subject>Down-Regulation - drug effects</subject><subject>Drug Tolerance</subject><subject>Malate dehydrogenase</subject><subject>Malate Dehydrogenase - drug effects</subject><subject>Malates</subject><issn>0162-0134</issn><issn>1873-3344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EokvbVwCfuCXYseskx9UKKFIlLnC2JvaknVViL3ZCxdvjaFdw7Om_fDP_zMfYBylqKaT5dKyPFGJ6HCjWjRBdLVQtRPOK7WTXqkoprV-zXSGbSkilr9i7nI9CiLs73b5lV7LTXdOafsee99M6U6B15hT86jBz9wThsSQFXhogkOPgyGc-4wJDnChvLD_EcUTgkGAgBzyv-YQhUwzc4TRl_kzLE_dUoIRhIZj4fqqWOGGC4PCGvRlhynh7yWv288vnH4f76uH712-H_UPllOmXqmlAYW8QpBQezahg7JxRXjf9MMIA3rRu0KqXGo1zvUMtlW_7UYAvfNepa_bxvPeU4q8V82JnytuBEDCu2ZpeybaRG9ieQZdizglHe0o0Q_pjpbCbc3u0_5zbzbkVyhbnZfL9pWIdZvT_5y6SC7A_A1ge_U2YbHaERYKnhG6xPtKLJX8B8Uialw</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Efraín Ramírez-Benítez, J.</creator><creator>Chee-González, Leticia</creator><creator>Teresa Hernandez-Sotomayor, S.M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080801</creationdate><title>Aluminium induces changes in organic acids metabolism in Coffea arabica suspension cells with differential Al-tolerance</title><author>Efraín Ramírez-Benítez, J. ; Chee-González, Leticia ; Teresa Hernandez-Sotomayor, S.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-22a3e96ea110de6f3af8c63d429bfabad67cb43914e6cc9ce413d79f0ad0de883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aluminium tolerance</topic><topic>Aluminum - pharmacology</topic><topic>Carboxylic Acids - metabolism</topic><topic>Citrate (si)-Synthase - drug effects</topic><topic>Citrate synthase</topic><topic>Coffea - cytology</topic><topic>Coffea - drug effects</topic><topic>Coffea - enzymology</topic><topic>Coffea - metabolism</topic><topic>Coffea arabica</topic><topic>Down-Regulation - drug effects</topic><topic>Drug Tolerance</topic><topic>Malate dehydrogenase</topic><topic>Malate Dehydrogenase - drug effects</topic><topic>Malates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Efraín Ramírez-Benítez, J.</creatorcontrib><creatorcontrib>Chee-González, Leticia</creatorcontrib><creatorcontrib>Teresa Hernandez-Sotomayor, S.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of inorganic biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Efraín Ramírez-Benítez, J.</au><au>Chee-González, Leticia</au><au>Teresa Hernandez-Sotomayor, S.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aluminium induces changes in organic acids metabolism in Coffea arabica suspension cells with differential Al-tolerance</atitle><jtitle>Journal of inorganic biochemistry</jtitle><addtitle>J Inorg Biochem</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>102</volume><issue>8</issue><spage>1631</spage><epage>1637</epage><pages>1631-1637</pages><issn>0162-0134</issn><eissn>1873-3344</eissn><abstract>The primary Al-tolerance mechanism in plants involves exudation and/or accumulation of specific organic acid species, which form non-phytotoxic complexes with Al
3+ under physiological conditions. An evaluation was done of the role of organic acids in the tolerance mechanism of a cell suspension line of coffee
Coffea arabica that exhibits Al-tolerance (LAMt) but for which the metabolic tolerance mechanism remains unknown. Significant differences existed in malate dehydrogenase and citrate synthase activities (key enzymes in organic acids metabolism) between protein extracts (day 7 of culture cycle) of the L2 (Al-sensitive) and LAMt (Al-tolerant) cells when cell suspensions were treated with 100
μM AlCl
3. HPLC analysis showed that the suspension cells of both lines exudate malate when incubated in a minimal solution but that exudation was not enhanced by treatment with AlCl
3 (100
μM). This is the first study demonstrating that plant Al-tolerance may be associated with down-regulation of malate dehydrogenase and citrate synthase activities.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18482769</pmid><doi>10.1016/j.jinorgbio.2008.03.002</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of inorganic biochemistry, 2008-08, Vol.102 (8), p.1631-1637 |
| issn | 0162-0134 1873-3344 |
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| subjects | Aluminium tolerance Aluminum - pharmacology Carboxylic Acids - metabolism Citrate (si)-Synthase - drug effects Citrate synthase Coffea - cytology Coffea - drug effects Coffea - enzymology Coffea - metabolism Coffea arabica Down-Regulation - drug effects Drug Tolerance Malate dehydrogenase Malate Dehydrogenase - drug effects Malates |
| title | Aluminium induces changes in organic acids metabolism in Coffea arabica suspension cells with differential Al-tolerance |
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