Leucine-rich glioma inactivated 3 is involved in amyloid β peptide uptake by astrocytes and endocytosis itself
We earlier showed that leucine-rich glioma inactivated 3 (LGI3) colocalizes with amyloid β peptide (Aβ) taken up by astrocytes both in vitro and in vivo, and that LGI3 accumulated with endocytosis-associated proteins in aged monkey brains. In this study, we confirmed that LGI3 localizes to the endoc...
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| Veröffentlicht in: | Neuroreport 2008-08, Vol.19 (12), p.1175-1179 |
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| creator | Okabayashi, Sachi Kimura, Nobuyuki |
| description | We earlier showed that leucine-rich glioma inactivated 3 (LGI3) colocalizes with amyloid β peptide (Aβ) taken up by astrocytes both in vitro and in vivo, and that LGI3 accumulated with endocytosis-associated proteins in aged monkey brains. In this study, we confirmed that LGI3 localizes to the endocytic pathway and found that its accumulation is caused by endocytic perturbation. Most notably, RNA interference experiments demonstrated that the downregulation of LGI3 clearly inhibited Aβ uptake by cultured rat astrocytes, moreover, transferrin uptake by both astrocytes and neuronal cells. Together with our earlier findings, our results suggest that LGI3 is involved in Aβ uptake by astrocytes and even endocytosis itself. |
| doi_str_mv | 10.1097/WNR.0b013e32830a4cc4 |
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In this study, we confirmed that LGI3 localizes to the endocytic pathway and found that its accumulation is caused by endocytic perturbation. Most notably, RNA interference experiments demonstrated that the downregulation of LGI3 clearly inhibited Aβ uptake by cultured rat astrocytes, moreover, transferrin uptake by both astrocytes and neuronal cells. 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Together with our earlier findings, our results suggest that LGI3 is involved in Aβ uptake by astrocytes and even endocytosis itself.</description><subject>Amyloid beta-Peptides - metabolism</subject><subject>Animals</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - metabolism</subject><subject>Blotting, Western</subject><subject>Cell Line, Tumor</subject><subject>Cells, Cultured</subject><subject>Endocytosis</subject><subject>Female</subject><subject>Immunohistochemistry</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA Interference</subject><subject>Transferrin - metabolism</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUV1rFDEUDaLYtfoPRPLk29SbyeRjHqVYFRYLxaJvIZPccWMzk3GS2bJ_qz_E3-QsXSjIfbicwzkH7rmEvGVwwaBVH358u7mADhhHXmsOtnGueUY2rFG8EkL_fE420Iq2alopzsirnH8DQAtMvyRnTMtaSwkbkra4uDBiNQe3o79iSIOlYbSuhL0t6CmnIa_EPsX9isJI7XCIKXj694FOOJXgkS5TsXdIuwO1uczJHQpmakdPcfRHlPIxo2SM_Wvyorcx45vTPie3V5--X36pttefv15-3FaOC6irXjItfKNAo-t6z8GhYNb3wjtQIBttlZQMa5Ci7tZjVGcVKpSgJdq6AX5O3j_mTnP6s2AuZgjZYYx2xLRkI1vOZMPUKmwehW5OOc_Ym2kOg50PhoE5Fm3Wos3_Ra-2d6f8pRvQP5lOzT7l3qdYcM53cbnH2ezQxrIz6yuAKSGrGkCvI6E6UjX_B7axjJE</recordid><startdate>20080806</startdate><enddate>20080806</enddate><creator>Okabayashi, Sachi</creator><creator>Kimura, Nobuyuki</creator><general>Lippincott Williams & Wilkins, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080806</creationdate><title>Leucine-rich glioma inactivated 3 is involved in amyloid β peptide uptake by astrocytes and endocytosis itself</title><author>Okabayashi, Sachi ; Kimura, Nobuyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3502-f6185d4708ecbfd30ce51adf5dc070648a7661e20652b8627ba7e7e6086ea2403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amyloid beta-Peptides - metabolism</topic><topic>Animals</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - metabolism</topic><topic>Blotting, Western</topic><topic>Cell Line, Tumor</topic><topic>Cells, Cultured</topic><topic>Endocytosis</topic><topic>Female</topic><topic>Immunohistochemistry</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA Interference</topic><topic>Transferrin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okabayashi, Sachi</creatorcontrib><creatorcontrib>Kimura, Nobuyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okabayashi, Sachi</au><au>Kimura, Nobuyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leucine-rich glioma inactivated 3 is involved in amyloid β peptide uptake by astrocytes and endocytosis itself</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>2008-08-06</date><risdate>2008</risdate><volume>19</volume><issue>12</issue><spage>1175</spage><epage>1179</epage><pages>1175-1179</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>We earlier showed that leucine-rich glioma inactivated 3 (LGI3) colocalizes with amyloid β peptide (Aβ) taken up by astrocytes both in vitro and in vivo, and that LGI3 accumulated with endocytosis-associated proteins in aged monkey brains. 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| identifier | ISSN: 0959-4965 |
| ispartof | Neuroreport, 2008-08, Vol.19 (12), p.1175-1179 |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Amyloid beta-Peptides - metabolism Animals Astrocytes - cytology Astrocytes - metabolism Blotting, Western Cell Line, Tumor Cells, Cultured Endocytosis Female Immunohistochemistry Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neurons - cytology Neurons - metabolism Pregnancy Rats Rats, Sprague-Dawley RNA Interference Transferrin - metabolism |
| title | Leucine-rich glioma inactivated 3 is involved in amyloid β peptide uptake by astrocytes and endocytosis itself |
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