D-Amino Acid Specific Proteases and Native All-L-Proteins: A Convenient Combination for Semisynthesis

Side reactions side‐stepped: A D‐amino acid specific protease was used for the first time in the chemoenzymatic synthesis of a native protein, the peptidyl prolyl cis/trans isomerase parvulin 10 from E. coli (see scheme). Side reactions that complicate synthesis with L‐amino acid specific enzymes ar...

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Veröffentlicht in:	Angewandte Chemie (International ed.) 2008-07, Vol.47 (29), p.5456-5460
Hauptverfasser:	Wehofsky, Nicole, Pech, Andreas, Liebscher, Sandra, Schmidt, Stephanie, Komeda, Hidenobu, Asano, Yasuhisa, Bordusa, Frank
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids Biomimetic Materials Escherichia coli Proteins - chemical synthesis Methods Peptide Hydrolases - metabolism peptide ligation Peptides - chemical synthesis Peptidylprolyl Isomerase - chemical synthesis proteases protein semisynthesis Proteins - chemical synthesis substrate mimetics
Online-Zugang:	Volltext
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