Membrane protein pattern in hereditary spherocytosis in five subjects from north-east Italy obtained by SDS-PAGE using N, N'-diallyltartardiamide
: In the present study we examined five subjects affected by hereditary spherocytosis (three unsplenectomized and two splenectomized), coming from an area in the north‐east of Italy where hereditary spherocytosis is an anaemic disease with very low incidence. All patients showed a low degree of spec...
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| Veröffentlicht in: | European journal of haematology 1999-11, Vol.63 (5), p.302-305 |
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| container_title | European journal of haematology |
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| creator | Giuliani, Anna Lisa Bigoni, Beatrice Veronesi, Massimo Manservigi, Roberto Mischiati, Carlo Berti, Gilberto Zavagli, Giorgio Ricci, Giorgio |
| description | : In the present study we examined five subjects affected by hereditary spherocytosis (three unsplenectomized and two splenectomized), coming from an area in the north‐east of Italy where hereditary spherocytosis is an anaemic disease with very low incidence. All patients showed a low degree of spectrin deficiency (14%), detected with sodium dodecyl sulfate polyacrylamide gel electrophoresis. Moreover, when this analysis was performed with N, N'‐diallyltartardiamide as cross‐linking agent instead of N, N'‐methylenbisacrylamide, some unusual bands appeared in the region between proteins 4.2 and 5, the three unsplenectomized and two splenectomized patients showing different patterns. We hypothesise that some alterations of proteins in this region (e.g. the 4.5 or 4.9 bands), possibly due to proteolysis, must have occurred in relation to the disease. |
| doi_str_mv | 10.1111/j.1600-0609.1999.tb01132.x |
| format | Article |
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All patients showed a low degree of spectrin deficiency (14%), detected with sodium dodecyl sulfate polyacrylamide gel electrophoresis. Moreover, when this analysis was performed with N, N'‐diallyltartardiamide as cross‐linking agent instead of N, N'‐methylenbisacrylamide, some unusual bands appeared in the region between proteins 4.2 and 5, the three unsplenectomized and two splenectomized patients showing different patterns. We hypothesise that some alterations of proteins in this region (e.g. the 4.5 or 4.9 bands), possibly due to proteolysis, must have occurred in relation to the disease.</description><identifier>ISSN: 0902-4441</identifier><identifier>EISSN: 1600-0609</identifier><identifier>DOI: 10.1111/j.1600-0609.1999.tb01132.x</identifier><identifier>PMID: 10580561</identifier><identifier>CODEN: EJHAEC</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; anaemia ; Anemias. 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All patients showed a low degree of spectrin deficiency (14%), detected with sodium dodecyl sulfate polyacrylamide gel electrophoresis. Moreover, when this analysis was performed with N, N'‐diallyltartardiamide as cross‐linking agent instead of N, N'‐methylenbisacrylamide, some unusual bands appeared in the region between proteins 4.2 and 5, the three unsplenectomized and two splenectomized patients showing different patterns. We hypothesise that some alterations of proteins in this region (e.g. the 4.5 or 4.9 bands), possibly due to proteolysis, must have occurred in relation to the disease.</description><subject>Adult</subject><subject>anaemia</subject><subject>Anemias. Hemoglobinopathies</subject><subject>Biological and medical sciences</subject><subject>Diseases of red blood cells</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Erythrocyte Membrane - metabolism</subject><subject>erythrocyte membrane proteins</subject><subject>Female</subject><subject>Hematologic and hematopoietic diseases</subject><subject>hereditary spherocytosis</subject><subject>Humans</subject><subject>Italy</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Proteins - analysis</subject><subject>Membrane Proteins - blood</subject><subject>Middle Aged</subject><subject>N'-diallyltartardiamide</subject><subject>N, N'‐diallyltartardiamide</subject><subject>spectrin</subject><subject>Spherocytosis, Hereditary - blood</subject><subject>Spherocytosis, Hereditary - genetics</subject><subject>Tartrates</subject><issn>0902-4441</issn><issn>1600-0609</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUdtu1DAQtRCIbhd-AVkIwQsJdpw4MS-oapdtq7JUahG8WY4zoV5yWWynbD6DP8ZRVoVXrJE8ozlzxj4HoZeUxDScd9uYckIiwomIqRAi9iWhlCXx_hFaPLQeowURJInSNKVH6Ni5LSEkETR_io4oyQqScbpAvz9BW1rVAd7Z3oPp8E55D7bDIb0DC5Xxyo7Y7ULR69H3zripV5t7wG4ot6C9w7XtW9z11t9FoJzHF141I-5Lr0wHFS5HfHN2E12frFd4cKb7jjdv8eZNVBnVNGMTNoQIRWsqeIae1Kpx8PxwL9GXj6vb0_Po6vP64vTkKtIpK5Ko4IniRc6TVJdUs6piqiIMVFJo0DklBQdeZTqva6gVz0qepUWdZnmmE6GTrGRL9HrmDR__OYDzsjVOQ9MEMfrBSS4YTTNaBOD7Gaht75yFWu6saYMokhI5GSK3clJdTqrLyRB5METuw_CLw5ahbKH6Z3R2IABeHQDKadXUwQtt3F-cEJyFWKIPM-yXaWD8jxfI1eX5lAWGaGYwzsP-gUHZH5LnLM_k181akrPLW_otvZaM_QFNErkz</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>Giuliani, Anna Lisa</creator><creator>Bigoni, Beatrice</creator><creator>Veronesi, Massimo</creator><creator>Manservigi, Roberto</creator><creator>Mischiati, Carlo</creator><creator>Berti, Gilberto</creator><creator>Zavagli, Giorgio</creator><creator>Ricci, Giorgio</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199911</creationdate><title>Membrane protein pattern in hereditary spherocytosis in five subjects from north-east Italy obtained by SDS-PAGE using N, N'-diallyltartardiamide</title><author>Giuliani, Anna Lisa ; Bigoni, Beatrice ; Veronesi, Massimo ; Manservigi, Roberto ; Mischiati, Carlo ; Berti, Gilberto ; Zavagli, Giorgio ; Ricci, Giorgio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4382-862a687624cb1c3dd3ad03ea28cec71086e6d5c7ffefa65b6548f4575c29c25b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>anaemia</topic><topic>Anemias. Hemoglobinopathies</topic><topic>Biological and medical sciences</topic><topic>Diseases of red blood cells</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Erythrocyte Membrane - metabolism</topic><topic>erythrocyte membrane proteins</topic><topic>Female</topic><topic>Hematologic and hematopoietic diseases</topic><topic>hereditary spherocytosis</topic><topic>Humans</topic><topic>Italy</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Proteins - analysis</topic><topic>Membrane Proteins - blood</topic><topic>Middle Aged</topic><topic>N'-diallyltartardiamide</topic><topic>N, N'‐diallyltartardiamide</topic><topic>spectrin</topic><topic>Spherocytosis, Hereditary - blood</topic><topic>Spherocytosis, Hereditary - genetics</topic><topic>Tartrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giuliani, Anna Lisa</creatorcontrib><creatorcontrib>Bigoni, Beatrice</creatorcontrib><creatorcontrib>Veronesi, Massimo</creatorcontrib><creatorcontrib>Manservigi, Roberto</creatorcontrib><creatorcontrib>Mischiati, Carlo</creatorcontrib><creatorcontrib>Berti, Gilberto</creatorcontrib><creatorcontrib>Zavagli, Giorgio</creatorcontrib><creatorcontrib>Ricci, Giorgio</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giuliani, Anna Lisa</au><au>Bigoni, Beatrice</au><au>Veronesi, Massimo</au><au>Manservigi, Roberto</au><au>Mischiati, Carlo</au><au>Berti, Gilberto</au><au>Zavagli, Giorgio</au><au>Ricci, Giorgio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Membrane protein pattern in hereditary spherocytosis in five subjects from north-east Italy obtained by SDS-PAGE using N, N'-diallyltartardiamide</atitle><jtitle>European journal of haematology</jtitle><addtitle>Eur J Haematol</addtitle><date>1999-11</date><risdate>1999</risdate><volume>63</volume><issue>5</issue><spage>302</spage><epage>305</epage><pages>302-305</pages><issn>0902-4441</issn><eissn>1600-0609</eissn><coden>EJHAEC</coden><abstract>: In the present study we examined five subjects affected by hereditary spherocytosis (three unsplenectomized and two splenectomized), coming from an area in the north‐east of Italy where hereditary spherocytosis is an anaemic disease with very low incidence. All patients showed a low degree of spectrin deficiency (14%), detected with sodium dodecyl sulfate polyacrylamide gel electrophoresis. Moreover, when this analysis was performed with N, N'‐diallyltartardiamide as cross‐linking agent instead of N, N'‐methylenbisacrylamide, some unusual bands appeared in the region between proteins 4.2 and 5, the three unsplenectomized and two splenectomized patients showing different patterns. We hypothesise that some alterations of proteins in this region (e.g. the 4.5 or 4.9 bands), possibly due to proteolysis, must have occurred in relation to the disease.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10580561</pmid><doi>10.1111/j.1600-0609.1999.tb01132.x</doi><tpages>4</tpages></addata></record> |
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| ispartof | European journal of haematology, 1999-11, Vol.63 (5), p.302-305 |
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| subjects | Adult anaemia Anemias. Hemoglobinopathies Biological and medical sciences Diseases of red blood cells Electrophoresis, Polyacrylamide Gel Erythrocyte Membrane - metabolism erythrocyte membrane proteins Female Hematologic and hematopoietic diseases hereditary spherocytosis Humans Italy Male Medical sciences Membrane Proteins - analysis Membrane Proteins - blood Middle Aged N'-diallyltartardiamide N, N'‐diallyltartardiamide spectrin Spherocytosis, Hereditary - blood Spherocytosis, Hereditary - genetics Tartrates |
| title | Membrane protein pattern in hereditary spherocytosis in five subjects from north-east Italy obtained by SDS-PAGE using N, N'-diallyltartardiamide |
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