The 2′-Hydroxyl Group of the Guanosine Nucleophile Donates a Functionally Important Hydrogen Bond in the Tetrahymena Ribozyme Reaction

In the first step of self-splicing, group I introns utilize an exogenous guanosine nucleophile to attack the 5′-splice site. Removal of the 2′-hydroxyl of this guanosine results in a 106-fold loss in activity, indicating that this functional group plays a critical role in catalysis. Biochemical and...

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Veröffentlicht in:	Biochemistry (Easton) 2008-07, Vol.47 (29), p.7684-7694
Hauptverfasser:	Hougland, James L, Sengupta, Raghuvir N, Dai, Qing, Deb, Shirshendu K, Piccirilli, Joseph A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, High Pressure Liquid Guanosine - chemistry Guanosine - metabolism Hydrogen Bonding Introns Molecular Structure RNA, Catalytic - chemistry RNA, Catalytic - metabolism Tetrahymena - enzymology
Online-Zugang:	Volltext
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