Selective Cloning of Cell Surface Proteins Involved in Organ Development: Epithelial Glycoprotein Is Involved in Normal Epithelial Differentiation
Coordinating the activities of neighboring cells during development in multicellular organisms requires complex cellular interactions involving secreted, cell surface, and extracellular matrix components. Although most cloning efforts have concentrated on secreted molecules, recent work has emphasiz...
Gespeichert in:
| Veröffentlicht in: | Endocrinology (Philadelphia) 1999-12, Vol.140 (12), p.5841-5854 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5854 |
|---|---|
| container_issue | 12 |
| container_start_page | 5841 |
| container_title | Endocrinology (Philadelphia) |
| container_volume | 140 |
| creator | Stephan, Jean-Philippe Roberts, Penelope E Bald, Laura Lee, James Gu, Qimin Devaux, Brigitte Mather, Jennie P |
| description | Coordinating the activities of neighboring cells during development in
multicellular organisms requires complex cellular interactions
involving secreted, cell surface, and extracellular matrix components.
Although most cloning efforts have concentrated on secreted molecules,
recent work has emphasized the importance of membrane-bound molecules
during development. To identify developmental genes, we raised
antibodies to normal embryonic pancreatic epithelial cell surface
proteins. These antibodies were characterized and used to clone the
genes coding for the proteins by a panning strategy. Using this
approach, we cloned the rat homologue of the mouse epithelial
glycoprotein (EGP). Our immunohistochemistry data, describing the
expression of EGP during rat development, as well as our in
vitro data, looking at the effect of the anti-EGP antibody and
the extracellular domain of EGP on embryonic pancreatic epithelial cell
number and volume, strongly suggest a role for EGP during pancreatic
development. |
| doi_str_mv | 10.1210/endo.140.12.7196 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69306735</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1210/endo.140.12.7196</oup_id><sourcerecordid>3130504397</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3646-a32c0152385c78f65168264ac67c9c57340a5359cfb12abf997146bc47ceb5be3</originalsourceid><addsrcrecordid>eNqNkV1rFDEYhYModq3eeyUBwRuZbTL52vFOtrUulFaoXodM9p2akknGZGahf6O_uBmmYBUEr8Ihzzmcl4PQW0rWtKbkBMI-rimf1VrRRj5DK9pwUSmqyHO0IoSyStW1OkKvcr4tknPOXqIjSoRqmCArdH8NHuzoDoC3PgYXbnDs8Ba8x9dT6owF_C3FEVzIeBcO0R9gj13AV-nGBHwKB_Bx6CGMn_DZ4Maf4J3x-Nzf2TgsPrz703kZU1-QJ_Sp6zpIJcOZ0cXwGr3ojM_w5vE9Rj--nH3ffq0urs53288XlWWSy8qw2hIqarYRVm06Kajc1JIbK5VtrFCMEyOYaGzX0tq0XdMoymVrubLQihbYMfqw5JaivybIo-5dtuVyEyBOWcuGEamYKOD7v8DbOKVQumlGGRGEs0YViiyUTTHnBJ0ekutNutOU6HktPa-ly1pF6XmtYnn3GDy1PeyfGJZ5CvBxAeI0_E-cWuj5xyYXYEiQ8--2_3Q-AC-csic</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3130504397</pqid></control><display><type>article</type><title>Selective Cloning of Cell Surface Proteins Involved in Organ Development: Epithelial Glycoprotein Is Involved in Normal Epithelial Differentiation</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Stephan, Jean-Philippe ; Roberts, Penelope E ; Bald, Laura ; Lee, James ; Gu, Qimin ; Devaux, Brigitte ; Mather, Jennie P</creator><creatorcontrib>Stephan, Jean-Philippe ; Roberts, Penelope E ; Bald, Laura ; Lee, James ; Gu, Qimin ; Devaux, Brigitte ; Mather, Jennie P</creatorcontrib><description>Coordinating the activities of neighboring cells during development in
multicellular organisms requires complex cellular interactions
involving secreted, cell surface, and extracellular matrix components.
Although most cloning efforts have concentrated on secreted molecules,
recent work has emphasized the importance of membrane-bound molecules
during development. To identify developmental genes, we raised
antibodies to normal embryonic pancreatic epithelial cell surface
proteins. These antibodies were characterized and used to clone the
genes coding for the proteins by a panning strategy. Using this
approach, we cloned the rat homologue of the mouse epithelial
glycoprotein (EGP). Our immunohistochemistry data, describing the
expression of EGP during rat development, as well as our in
vitro data, looking at the effect of the anti-EGP antibody and
the extracellular domain of EGP on embryonic pancreatic epithelial cell
number and volume, strongly suggest a role for EGP during pancreatic
development.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/endo.140.12.7196</identifier><identifier>PMID: 10579350</identifier><language>eng</language><publisher>United States: Endocrine Society</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies ; Antibodies, Monoclonal ; Antigens, Neoplasm - analysis ; Antigens, Neoplasm - chemistry ; Antigens, Neoplasm - genetics ; Antigens, Surface - analysis ; Base Sequence ; Cell Adhesion Molecules - analysis ; Cell Adhesion Molecules - chemistry ; Cell Adhesion Molecules - genetics ; Cell Differentiation ; Cell Line ; Cell number ; Cell surface ; Cloning ; Cloning, Molecular ; DNA - chemistry ; Embryogenesis ; Epithelial Cell Adhesion Molecule ; Epithelial cells ; Epithelial Cells - cytology ; Epithelium ; Extracellular matrix ; Genes ; Glycoproteins ; Humans ; Immunohistochemistry ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - physiology ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Pancreas ; Pancreas - cytology ; Pancreas - embryology ; Pancreatic Ducts - cytology ; Pancreatic Ducts - embryology ; Panning ; Proteins ; Rats ; Recombinant Fusion Proteins - pharmacology</subject><ispartof>Endocrinology (Philadelphia), 1999-12, Vol.140 (12), p.5841-5854</ispartof><rights>Copyright © 1999 by The Endocrine Society 1999</rights><rights>Copyright © 1999 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3646-a32c0152385c78f65168264ac67c9c57340a5359cfb12abf997146bc47ceb5be3</citedby><cites>FETCH-LOGICAL-c3646-a32c0152385c78f65168264ac67c9c57340a5359cfb12abf997146bc47ceb5be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10579350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stephan, Jean-Philippe</creatorcontrib><creatorcontrib>Roberts, Penelope E</creatorcontrib><creatorcontrib>Bald, Laura</creatorcontrib><creatorcontrib>Lee, James</creatorcontrib><creatorcontrib>Gu, Qimin</creatorcontrib><creatorcontrib>Devaux, Brigitte</creatorcontrib><creatorcontrib>Mather, Jennie P</creatorcontrib><title>Selective Cloning of Cell Surface Proteins Involved in Organ Development: Epithelial Glycoprotein Is Involved in Normal Epithelial Differentiation</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>Coordinating the activities of neighboring cells during development in
multicellular organisms requires complex cellular interactions
involving secreted, cell surface, and extracellular matrix components.
Although most cloning efforts have concentrated on secreted molecules,
recent work has emphasized the importance of membrane-bound molecules
during development. To identify developmental genes, we raised
antibodies to normal embryonic pancreatic epithelial cell surface
proteins. These antibodies were characterized and used to clone the
genes coding for the proteins by a panning strategy. Using this
approach, we cloned the rat homologue of the mouse epithelial
glycoprotein (EGP). Our immunohistochemistry data, describing the
expression of EGP during rat development, as well as our in
vitro data, looking at the effect of the anti-EGP antibody and
the extracellular domain of EGP on embryonic pancreatic epithelial cell
number and volume, strongly suggest a role for EGP during pancreatic
development.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Neoplasm - chemistry</subject><subject>Antigens, Neoplasm - genetics</subject><subject>Antigens, Surface - analysis</subject><subject>Base Sequence</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Cell Adhesion Molecules - chemistry</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cell number</subject><subject>Cell surface</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>DNA - chemistry</subject><subject>Embryogenesis</subject><subject>Epithelial Cell Adhesion Molecule</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelium</subject><subject>Extracellular matrix</subject><subject>Genes</subject><subject>Glycoproteins</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - physiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Pancreas</subject><subject>Pancreas - cytology</subject><subject>Pancreas - embryology</subject><subject>Pancreatic Ducts - cytology</subject><subject>Pancreatic Ducts - embryology</subject><subject>Panning</subject><subject>Proteins</subject><subject>Rats</subject><subject>Recombinant Fusion Proteins - pharmacology</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1rFDEYhYModq3eeyUBwRuZbTL52vFOtrUulFaoXodM9p2akknGZGahf6O_uBmmYBUEr8Ihzzmcl4PQW0rWtKbkBMI-rimf1VrRRj5DK9pwUSmqyHO0IoSyStW1OkKvcr4tknPOXqIjSoRqmCArdH8NHuzoDoC3PgYXbnDs8Ba8x9dT6owF_C3FEVzIeBcO0R9gj13AV-nGBHwKB_Bx6CGMn_DZ4Maf4J3x-Nzf2TgsPrz703kZU1-QJ_Sp6zpIJcOZ0cXwGr3ojM_w5vE9Rj--nH3ffq0urs53288XlWWSy8qw2hIqarYRVm06Kajc1JIbK5VtrFCMEyOYaGzX0tq0XdMoymVrubLQihbYMfqw5JaivybIo-5dtuVyEyBOWcuGEamYKOD7v8DbOKVQumlGGRGEs0YViiyUTTHnBJ0ekutNutOU6HktPa-ly1pF6XmtYnn3GDy1PeyfGJZ5CvBxAeI0_E-cWuj5xyYXYEiQ8--2_3Q-AC-csic</recordid><startdate>199912</startdate><enddate>199912</enddate><creator>Stephan, Jean-Philippe</creator><creator>Roberts, Penelope E</creator><creator>Bald, Laura</creator><creator>Lee, James</creator><creator>Gu, Qimin</creator><creator>Devaux, Brigitte</creator><creator>Mather, Jennie P</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199912</creationdate><title>Selective Cloning of Cell Surface Proteins Involved in Organ Development: Epithelial Glycoprotein Is Involved in Normal Epithelial Differentiation</title><author>Stephan, Jean-Philippe ; Roberts, Penelope E ; Bald, Laura ; Lee, James ; Gu, Qimin ; Devaux, Brigitte ; Mather, Jennie P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3646-a32c0152385c78f65168264ac67c9c57340a5359cfb12abf997146bc47ceb5be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Neoplasm - chemistry</topic><topic>Antigens, Neoplasm - genetics</topic><topic>Antigens, Surface - analysis</topic><topic>Base Sequence</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Cell Adhesion Molecules - chemistry</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cell number</topic><topic>Cell surface</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>DNA - chemistry</topic><topic>Embryogenesis</topic><topic>Epithelial Cell Adhesion Molecule</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelium</topic><topic>Extracellular matrix</topic><topic>Genes</topic><topic>Glycoproteins</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - physiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Sequence Data</topic><topic>Pancreas</topic><topic>Pancreas - cytology</topic><topic>Pancreas - embryology</topic><topic>Pancreatic Ducts - cytology</topic><topic>Pancreatic Ducts - embryology</topic><topic>Panning</topic><topic>Proteins</topic><topic>Rats</topic><topic>Recombinant Fusion Proteins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stephan, Jean-Philippe</creatorcontrib><creatorcontrib>Roberts, Penelope E</creatorcontrib><creatorcontrib>Bald, Laura</creatorcontrib><creatorcontrib>Lee, James</creatorcontrib><creatorcontrib>Gu, Qimin</creatorcontrib><creatorcontrib>Devaux, Brigitte</creatorcontrib><creatorcontrib>Mather, Jennie P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stephan, Jean-Philippe</au><au>Roberts, Penelope E</au><au>Bald, Laura</au><au>Lee, James</au><au>Gu, Qimin</au><au>Devaux, Brigitte</au><au>Mather, Jennie P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective Cloning of Cell Surface Proteins Involved in Organ Development: Epithelial Glycoprotein Is Involved in Normal Epithelial Differentiation</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>1999-12</date><risdate>1999</risdate><volume>140</volume><issue>12</issue><spage>5841</spage><epage>5854</epage><pages>5841-5854</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><abstract>Coordinating the activities of neighboring cells during development in
multicellular organisms requires complex cellular interactions
involving secreted, cell surface, and extracellular matrix components.
Although most cloning efforts have concentrated on secreted molecules,
recent work has emphasized the importance of membrane-bound molecules
during development. To identify developmental genes, we raised
antibodies to normal embryonic pancreatic epithelial cell surface
proteins. These antibodies were characterized and used to clone the
genes coding for the proteins by a panning strategy. Using this
approach, we cloned the rat homologue of the mouse epithelial
glycoprotein (EGP). Our immunohistochemistry data, describing the
expression of EGP during rat development, as well as our in
vitro data, looking at the effect of the anti-EGP antibody and
the extracellular domain of EGP on embryonic pancreatic epithelial cell
number and volume, strongly suggest a role for EGP during pancreatic
development.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>10579350</pmid><doi>10.1210/endo.140.12.7196</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0013-7227 |
| ispartof | Endocrinology (Philadelphia), 1999-12, Vol.140 (12), p.5841-5854 |
| issn | 0013-7227 1945-7170 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69306735 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Animals Antibodies Antibodies, Monoclonal Antigens, Neoplasm - analysis Antigens, Neoplasm - chemistry Antigens, Neoplasm - genetics Antigens, Surface - analysis Base Sequence Cell Adhesion Molecules - analysis Cell Adhesion Molecules - chemistry Cell Adhesion Molecules - genetics Cell Differentiation Cell Line Cell number Cell surface Cloning Cloning, Molecular DNA - chemistry Embryogenesis Epithelial Cell Adhesion Molecule Epithelial cells Epithelial Cells - cytology Epithelium Extracellular matrix Genes Glycoproteins Humans Immunohistochemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - physiology Mice Mice, Inbred BALB C Molecular Sequence Data Pancreas Pancreas - cytology Pancreas - embryology Pancreatic Ducts - cytology Pancreatic Ducts - embryology Panning Proteins Rats Recombinant Fusion Proteins - pharmacology |
| title | Selective Cloning of Cell Surface Proteins Involved in Organ Development: Epithelial Glycoprotein Is Involved in Normal Epithelial Differentiation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T09%3A15%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Selective%20Cloning%20of%20Cell%20Surface%20Proteins%20Involved%20in%20Organ%20Development:%20Epithelial%20Glycoprotein%20Is%20Involved%20in%20Normal%20Epithelial%20Differentiation&rft.jtitle=Endocrinology%20(Philadelphia)&rft.au=Stephan,%20Jean-Philippe&rft.date=1999-12&rft.volume=140&rft.issue=12&rft.spage=5841&rft.epage=5854&rft.pages=5841-5854&rft.issn=0013-7227&rft.eissn=1945-7170&rft_id=info:doi/10.1210/endo.140.12.7196&rft_dat=%3Cproquest_cross%3E3130504397%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3130504397&rft_id=info:pmid/10579350&rft_oup_id=10.1210/endo.140.12.7196&rfr_iscdi=true |